##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547175_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1100266
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.663518640037953	31.0	30.0	31.0	28.0	34.0
2	31.01117366164182	31.0	31.0	33.0	28.0	34.0
3	31.091475152372244	31.0	31.0	34.0	28.0	34.0
4	34.92996966188176	35.0	35.0	37.0	32.0	37.0
5	34.76083510714682	35.0	35.0	37.0	32.0	37.0
6	34.805590648079644	35.0	35.0	37.0	32.0	37.0
7	35.49286627052004	36.0	35.0	37.0	35.0	37.0
8	34.47570678363232	37.0	35.0	37.0	33.0	37.0
9	36.590589911894035	39.0	37.0	39.0	32.0	39.0
10	36.022122832115144	38.0	35.0	39.0	30.0	39.0
11	35.74846173561666	37.0	35.0	39.0	30.0	39.0
12	35.63825929366171	37.0	35.0	39.0	30.0	39.0
13	35.60368220048606	37.0	35.0	39.0	30.0	39.0
14	36.57179627471902	38.0	35.0	40.0	30.0	41.0
15	36.67712353194591	38.0	35.0	40.0	31.0	41.0
16	36.68705113127189	38.0	35.0	40.0	31.0	41.0
17	36.6019598896994	38.0	35.0	40.0	31.0	41.0
18	36.41684283618689	38.0	35.0	40.0	30.0	41.0
19	36.32181672431939	38.0	35.0	40.0	30.0	41.0
20	36.10906089981877	38.0	34.0	40.0	30.0	41.0
21	35.95862909514608	38.0	34.0	40.0	30.0	41.0
22	35.917183662859706	38.0	34.0	40.0	30.0	41.0
23	35.787148744030986	37.0	34.0	40.0	29.0	41.0
24	35.678235081334876	37.0	34.0	40.0	29.0	41.0
25	35.49704071560877	37.0	34.0	40.0	29.0	41.0
26	35.32915131431854	37.0	34.0	40.0	27.0	41.0
27	35.15403820530672	37.0	33.0	40.0	27.0	41.0
28	35.15902245457008	37.0	34.0	40.0	27.0	41.0
29	35.01803836526803	37.0	33.0	40.0	27.0	41.0
30	34.953340374055	37.0	33.0	40.0	26.0	41.0
31	34.811291996662625	37.0	33.0	40.0	25.0	41.0
32	34.394131964452235	37.0	33.0	40.0	24.0	41.0
33	34.043562193142385	37.0	33.0	40.0	22.0	41.0
34	33.75922186089546	37.0	33.0	40.0	20.0	41.0
35	33.438686644865875	37.0	32.0	40.0	18.0	41.0
36	33.32213119372952	37.0	32.0	40.0	18.0	41.0
37	33.30468086808099	37.0	32.0	40.0	17.0	41.0
38	33.30228508378883	37.0	32.0	40.0	17.0	41.0
39	33.18572145281232	37.0	31.0	40.0	16.0	41.0
40	33.15175693877662	37.0	31.0	40.0	16.0	41.0
41	33.150370910307146	37.0	31.0	40.0	15.0	41.0
42	33.00679108506488	37.0	31.0	40.0	15.0	41.0
43	33.05517393066768	37.0	31.0	40.0	15.0	41.0
44	32.76764527850538	36.0	31.0	40.0	15.0	41.0
45	32.71660762033908	36.0	31.0	40.0	15.0	41.0
46	32.67551119456568	36.0	31.0	40.0	15.0	41.0
47	32.60806932141864	36.0	31.0	40.0	14.0	41.0
48	32.59161784513927	36.0	31.0	40.0	14.0	41.0
49	32.593052043778506	36.0	31.0	40.0	14.0	41.0
50	32.06790267080869	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	6.0
14	13.0
15	53.0
16	178.0
17	465.0
18	1041.0
19	2019.0
20	3579.0
21	5345.0
22	7843.0
23	11034.0
24	15099.0
25	21433.0
26	29177.0
27	33146.0
28	33937.0
29	35318.0
30	39351.0
31	45791.0
32	54768.0
33	66544.0
34	86021.0
35	98510.0
36	99571.0
37	117212.0
38	143930.0
39	148879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.605446319344598	23.890495571070996	26.676276464055054	20.827781645529353
2	35.91231574910067	24.664944658837047	26.896950373818697	12.525789218243588
3	26.075330874533975	24.926426882226664	36.30385743083945	12.694384812399912
4	23.22656521241227	27.214782607115005	35.84551372122741	13.713138459245311
5	20.525945544077523	31.234356055717438	35.08978737868843	13.149911021516614
6	19.172181999625547	39.32085513866647	32.16513097741819	9.3418318842898
7	83.99459767001798	2.4860351951255426	12.471166063479195	1.0482010713772851
8	82.37980633774015	4.8722763404485825	11.693263265428541	1.0546540563827291
9	79.16949174108807	5.24445906717103	13.551904721221957	2.0341444705189473
10	32.282011804418204	38.54477008287087	19.278429034433493	9.894789078277434
11	23.29136772380497	25.121925061757793	36.35366356862795	15.233043645809285
12	23.432697184135474	23.572390676436424	36.23687362874068	16.758038510687413
13	20.368619951902538	27.1896068768825	36.501173352625635	15.940599818589323
14	17.97210856283844	29.859506701106824	35.48241970578024	16.685965030274495
15	17.245647870605836	26.30472994712188	40.65953142240149	15.790090759870795
16	19.952356975495018	24.710842650777177	39.176162855164115	16.160637518563693
17	19.96571738106967	24.067634553826075	35.56203681655163	20.404611248552623
18	20.591202491033986	24.29148951253606	37.29089147533415	17.82641652109581
19	22.080751381938548	26.070241196219822	34.303977401828284	17.545030020013343
20	24.199875302881306	25.260346134480205	33.858176113776125	16.681602448862364
21	22.227443181921462	25.480656495792836	35.252747971854085	17.03915235043162
22	21.757556808989825	23.849323708994007	35.048161081047674	19.344958400968494
23	18.898975338690825	27.41637022320057	34.61744705371246	19.067207384396138
24	18.5142501904085	26.11141305829681	37.905379244655386	17.468957506639306
25	18.498435832789525	27.682305915115073	35.77580330574607	18.04345494634934
26	18.984772773129407	28.722872469021127	34.24453723008799	18.04781752776147
27	18.281760955987007	27.801731581272165	35.41379993565192	18.502707527088905
28	17.560117280730296	27.391739815644577	37.37723423244925	17.67090867117588
29	18.515340835761535	25.340054132364354	37.116660880187155	19.027944151686956
30	19.090747146599096	26.335722452570558	36.44191495511085	18.13161544571949
31	21.091081611174026	26.779524224142158	33.53752637998448	18.591867784699335
32	21.239409379186487	26.899404325863017	33.973875408310356	17.88731088664014
33	20.760979617656094	27.677398011026426	32.83333303037629	18.72828934094119
34	19.346867030336302	28.051852915567686	33.11417420878224	19.48710584531377
35	19.422030672582814	28.57899816953355	32.27655857765304	19.7224125802306
36	21.783823184575365	28.855476766527367	30.69439571885344	18.666304330043825
37	20.101957163086016	29.961300267389884	31.359325835752443	18.577416733771653
38	20.12104345676409	30.0449164111224	30.993505206922688	18.84053492519082
39	20.157489188977937	30.191971759556324	30.683852813774127	18.96668623769161
40	21.60068565237861	28.059305658813415	30.777557427022195	19.56245126178579
41	19.220897492061013	28.747684650802626	31.08720982017076	20.944208036965605
42	20.29990929466147	29.416432026437246	29.60874915702203	20.674909521879254
43	20.46768690480302	28.835572488834515	30.575515375372863	20.121225230989598
44	20.40015778002774	29.578665522700874	30.55597464613103	19.46520205114036
45	19.535548676410976	30.490445037836306	29.583755201015027	20.39025108473769
46	20.20365984225633	29.93476123046609	30.295673955207196	19.565904972070392
47	20.36434825760316	28.946727427731112	30.67176482777801	20.017159486887717
48	20.75843477849902	27.971236046556015	31.300521873801422	19.96980730114354
49	20.4886818278489	27.54088556767182	31.382774710842646	20.587657893636628
50	19.181725146464583	29.910039935797343	31.14792241148959	19.76031250624849
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	689.0
1	926.0
2	1163.0
3	12207.5
4	23252.0
5	17682.5
6	12113.0
7	12191.0
8	12269.0
9	12480.5
10	12692.0
11	12734.5
12	12777.0
13	12472.5
14	12168.0
15	11633.0
16	11098.0
17	10516.5
18	9935.0
19	9482.5
20	9030.0
21	9191.5
22	9353.0
23	9952.5
24	10552.0
25	11883.0
26	13214.0
27	15891.0
28	18568.0
29	21425.0
30	24282.0
31	27759.0
32	31236.0
33	35400.5
34	39565.0
35	44098.5
36	48632.0
37	51624.5
38	54617.0
39	58958.0
40	63299.0
41	69768.0
42	76237.0
43	79262.5
44	82288.0
45	83055.0
46	83822.0
47	83556.5
48	83291.0
49	80072.5
50	76854.0
51	71209.0
52	65564.0
53	59397.5
54	53231.0
55	47693.0
56	42155.0
57	37451.5
58	32748.0
59	29486.5
60	26225.0
61	22500.0
62	18775.0
63	15469.5
64	12164.0
65	9495.0
66	6826.0
67	5230.5
68	3635.0
69	3071.5
70	2508.0
71	1958.5
72	1409.0
73	1115.0
74	821.0
75	693.5
76	566.0
77	489.5
78	413.0
79	264.5
80	116.0
81	95.5
82	75.0
83	47.0
84	19.0
85	16.0
86	13.0
87	8.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1100266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.5804286938488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21856983909191	27.515275304521886
2	7.178339838336124	5.251734971929375
3	3.1338406864177206	3.4391170730215683
4	2.005951046642565	2.935141969002389
5	1.5500882567123493	2.8351446471919246
6	1.244873922001374	2.7322813041961895
7	1.047219585799383	2.6815418969595344
8	0.9306042702992068	2.723352252149706
9	0.8176587710747462	2.691927753407982
>10	6.808113218937882	44.18992724530081
>50	0.05542312171049771	1.2495189970194192
>100	0.008336659529201769	0.4714300822095267
>500	7.355875852844981E-4	0.14898123133478133
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.4519586176149937E-4	1.1346252717549572
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12650	1.1497219763220894	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013451292687404682	0.0
2	0.0	0.0	0.0	0.05144210581804764	0.0
3	0.0	0.0	0.0	0.10488372811665543	0.0
4	0.0	0.0	0.0	0.15441720456689564	0.0
5	0.0	0.0	0.0	0.24939423739350303	0.0
6	0.0	0.0	0.0	0.4909721830902709	0.0
7	0.0	0.0	0.0	0.6175779311548298	0.0
8	0.0	0.0	0.0	0.926775888739632	0.0
9	0.0	0.0	0.0	1.128272617712444	0.0
10	0.0	0.0	0.0	1.3711229829877503	0.0
11	0.0	0.0	0.0	1.5105438139504448	0.0
12	0.0	0.0	0.0	1.6500555320258918	0.0
13	0.0	0.0	0.0	1.7484862751371033	0.0
14	0.0	0.0	0.0	1.80256410722498	0.0
15	0.0	0.0	0.0	1.8479167764885946	0.0
16	0.0	0.0	0.0	1.9139917074598325	0.0
17	0.0	0.0	0.0	1.9896097852701073	0.0
18	0.0	0.0	0.0	2.0994923045881633	0.0
19	0.0	0.0	0.0	2.1568420727351385	0.0
20	0.0	0.0	0.0	2.2194632934217724	0.0
21	0.0	0.0	0.0	2.2823571754466645	0.0
22	0.0	0.0	0.0	2.359156785722725	0.0
23	0.0	0.0	0.0	2.441682284102208	0.0
24	0.0	0.0	0.0	2.509120521764737	0.0
25	0.0	0.0	0.0	2.565924967235196	0.0
26	0.0	0.0	0.0	2.6254560260882367	0.0
27	0.0	0.0	0.0	2.697256845162897	0.0
28	0.0	0.0	0.0	2.772874922973172	0.0
29	0.0	0.0	0.0	2.8514922755042873	0.0
30	0.0	0.0	0.0	2.953831164463866	0.0
31	0.0	0.0	0.0	3.043082309187051	0.0
32	0.0	0.0	0.0	3.1335149863760217	0.0
33	0.0	0.0	0.0	3.222402582648196	0.0
34	0.0	0.0	0.0	3.3181067123768253	0.0
35	0.0	0.0	0.0	3.418446084855844	0.0
36	0.0	0.0	0.0	3.509696746059589	0.0
37	0.0	0.0	0.0	3.612035635019168	0.0
38	0.0	0.0	0.0	3.7189188796163837	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	20	7.856334E-4	44.000004	38
CGACGGT	30	2.5276167E-6	44.000004	28
GTAATCG	20	7.856334E-4	44.000004	1
AATCGCG	30	2.5276167E-6	44.000004	1
ACGGGTC	45	4.802132E-10	44.0	5
CGTTTTT	11390	0.0	43.053555	1
TCGTTGA	60	3.6379788E-12	40.333336	24
CGCCGGT	60	3.6379788E-12	40.333336	28
TACGGGA	410	0.0	39.17073	4
ACGGGCG	135	0.0	39.11111	5
CGTTAGG	90	0.0	39.11111	2
GCTAACG	45	2.3508619E-8	39.11111	1
AGGGCGA	1110	0.0	38.648647	6
GCGTTAG	40	4.124322E-7	38.500004	1
CAACGGG	185	0.0	38.054054	3
ATAGGGA	1920	0.0	37.927086	4
ACGGGAA	430	0.0	37.860466	5
GTTTTTT	12820	0.0	37.633385	2
AATGCGG	170	0.0	37.52941	2
GGGCGAT	2170	0.0	37.308758	7
>>END_MODULE