FastQCFastQC Report
Thu 26 May 2016
SRR1547174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547174_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2038323
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250891.2308647844330853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT227650.043.2268831
TAGGGAC17400.038.563225
TACGGGA6400.038.54
GTTTTTT252500.038.4586142
AGGGCGA20300.036.9556666
TATCCGT301.3014486E-436.66666444
GGGCGAT40050.036.63927
GCGCGAC9650.036.4766859
CGGTCTA1400.036.14285731
ATAGGGA22850.035.8161934
CACGACG1600.035.7526
ATAGGGC10100.035.7227754
ACGGGAC5050.035.7227755
TAGGGAT26850.035.4785845
TAAGGGA23850.035.4213834
CGCGACC10000.035.42000210
ACGGGAT8700.035.40235
CCGCTGA9600.035.29166436
CGGGATT6050.035.2727286
CGACCCG9550.035.24607532
Produced by FastQC (version 0.11.5)