##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547174_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2038323
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.55055160541288	31.0	30.0	31.0	27.0	34.0
2	30.89681076061056	31.0	31.0	33.0	28.0	34.0
3	30.980850925000603	31.0	31.0	34.0	28.0	34.0
4	34.83543972177128	35.0	35.0	37.0	32.0	37.0
5	34.66020007623914	35.0	35.0	37.0	32.0	37.0
6	34.701200447622874	35.0	35.0	37.0	31.0	37.0
7	35.38337496069072	35.0	35.0	37.0	33.0	37.0
8	34.36069847614927	35.0	35.0	37.0	32.0	37.0
9	36.46003258561082	39.0	37.0	39.0	32.0	39.0
10	35.84383583956026	38.0	35.0	39.0	30.0	39.0
11	35.62019954639182	37.0	35.0	39.0	30.0	39.0
12	35.425870188385254	37.0	35.0	39.0	30.0	39.0
13	35.381385580204906	37.0	35.0	39.0	30.0	39.0
14	36.30492272323866	38.0	35.0	40.0	30.0	41.0
15	36.39941657921733	38.0	35.0	40.0	30.0	41.0
16	36.42442684500935	38.0	35.0	40.0	30.0	41.0
17	36.31171114685945	38.0	34.0	40.0	30.0	41.0
18	36.14755610371859	38.0	34.0	40.0	30.0	41.0
19	36.00678351762699	37.0	34.0	40.0	29.0	41.0
20	35.77653002002136	37.0	34.0	40.0	29.0	41.0
21	35.624099811462656	37.0	34.0	40.0	29.0	41.0
22	35.59035000831566	37.0	34.0	40.0	29.0	41.0
23	35.43909184167573	37.0	34.0	40.0	28.0	41.0
24	35.294468541050655	37.0	34.0	40.0	27.0	41.0
25	35.095094349619764	37.0	33.0	40.0	27.0	41.0
26	34.922150709185935	36.0	33.0	40.0	27.0	41.0
27	34.73290641375287	36.0	33.0	40.0	26.0	41.0
28	34.71192740306615	36.0	33.0	40.0	25.0	41.0
29	34.50605276985051	36.0	33.0	40.0	25.0	41.0
30	34.405055037891444	36.0	33.0	40.0	24.0	41.0
31	34.262129211121106	36.0	33.0	40.0	24.0	41.0
32	33.83081091662116	36.0	32.0	40.0	22.0	41.0
33	33.43362803638089	36.0	32.0	40.0	20.0	41.0
34	33.08428644527879	36.0	31.0	40.0	18.0	41.0
35	32.7529297368474	36.0	31.0	40.0	16.0	41.0
36	32.623358025200126	36.0	31.0	40.0	15.0	41.0
37	32.609435305395664	36.0	31.0	40.0	15.0	41.0
38	32.58101880810843	36.0	31.0	40.0	15.0	41.0
39	32.4554135924483	36.0	30.0	40.0	14.0	41.0
40	32.378883032767625	36.0	30.0	40.0	14.0	41.0
41	32.37486404264682	36.0	30.0	40.0	13.0	41.0
42	32.21086893490384	35.0	30.0	40.0	12.0	41.0
43	32.23941298802987	35.0	30.0	40.0	12.0	41.0
44	31.850581581034998	35.0	30.0	39.0	10.0	41.0
45	31.822474161357153	35.0	30.0	39.0	10.0	41.0
46	31.78086986213667	35.0	29.0	39.0	10.0	41.0
47	31.734664721930724	35.0	29.0	39.0	10.0	41.0
48	31.708287646266072	35.0	29.0	39.0	10.0	41.0
49	31.70281255718549	35.0	29.0	39.0	10.0	41.0
50	31.19045754769975	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	3.0
12	5.0
13	12.0
14	66.0
15	172.0
16	450.0
17	1273.0
18	2716.0
19	5290.0
20	8500.0
21	12815.0
22	17832.0
23	24909.0
24	33566.0
25	46765.0
26	61474.0
27	69782.0
28	70612.0
29	72364.0
30	79643.0
31	91260.0
32	106456.0
33	127405.0
34	163091.0
35	180314.0
36	181690.0
37	208611.0
38	242645.0
39	228600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.587497663520452	22.512477168731355	25.607619597090352	21.29240557065784
2	37.01174936455116	23.877030284209127	26.461360638132426	12.649859713107295
3	26.969621595792226	24.127383147813177	36.33212204346416	12.570873212930433
4	23.409390955211713	26.724370965739976	35.96412344854079	13.902114630507532
5	20.964047405636887	30.34828140584196	35.3573010754429	13.330370113078253
6	19.087651957025457	38.87406461095714	32.58335406115714	9.454929370860262
7	82.44571640510361	2.934225831725394	13.492954747603788	1.127103015567209
8	80.62750604295786	5.17209490350646	12.894865043469558	1.3055340100661181
9	77.87720591878717	4.834268170451886	14.873992002248906	2.4145339085120465
10	34.68567052424959	31.262905829939612	21.688662689868092	12.362760955942703
11	26.79786275286105	24.848122696942536	32.718612310217765	15.63540223997865
12	25.10141915682647	22.327275902788717	34.9924913764894	17.57881356389542
13	22.044249120477964	24.601105909122353	35.96839166314661	17.386253307253067
14	19.986528141025737	26.68046232123172	35.95288872273923	17.380120815003313
15	19.18120925878774	23.929916897371026	39.33969248249664	17.549181361344594
16	21.413093018133043	22.520915478067018	38.39641705460813	17.66957444919181
17	21.461907656441102	22.121469462886893	35.675356653484265	20.74126622718774
18	22.55304973745574	22.74811205093599	36.23743636312792	18.461401848480342
19	24.41070428975192	24.666404686597758	32.966904656425896	17.95598636722443
20	25.70186373798461	24.260335579787895	32.583599360847124	17.45420132138037
21	23.785680679656757	24.873437624949528	34.02291982183393	17.317961873559785
22	22.930811260040727	22.43383408812048	34.333174869733604	20.302179782105192
23	21.11093286000305	25.467700653919913	33.748576648548834	19.672789837528203
24	20.77732528161631	24.167955716537566	36.94247673209791	18.11224226974822
25	21.109559181739108	25.225197380395553	34.483985119139604	19.181258318725735
26	19.874377122762194	26.470436726662065	33.87686838641373	19.778317764162008
27	19.95925081549882	25.15877022434619	34.55227655283289	20.3297024073221
28	19.70963385096474	25.66796332082796	36.13436143339402	18.488041394813283
29	20.74357204427365	24.186500373100827	35.73354173994995	19.336385842675572
30	20.919402862058664	24.685783362106985	35.530286416824026	18.864527359010324
31	23.052087426771912	24.95492618196429	33.028523938551444	18.964462452712354
32	23.353904165335916	24.7811068216372	32.73337935155517	19.13160966147171
33	23.482244963138815	25.480603417613402	31.00754885265976	20.029602766588024
34	21.291571551711876	26.88877081797144	31.731428237820992	20.088229392495695
35	21.27126073738068	26.990815489007385	31.351704317716084	20.38621945589585
36	23.06140881499154	27.963330639942736	28.947522056121628	20.027738488944095
37	21.952605156297604	28.97504468133853	29.2325602958903	19.839789866473566
38	22.45017104747383	28.907243847025228	28.840620451223874	19.80196465427707
39	22.370988307544977	27.916968998534582	28.447257868355507	21.264784825564938
40	22.852953138437822	26.64464856649314	29.761033948005295	20.741364347063737
41	21.253059500383404	26.445514278159056	29.753380597677605	22.54804562377994
42	21.911100448751252	27.798587368145284	28.14951310464534	22.140799078458127
43	21.370803351578722	27.13593478560562	29.416535063382987	22.076726799432674
44	21.572145337122723	27.430147233779927	29.86283331935125	21.1348741097461
45	21.10990260130509	28.042120900367607	29.39377125215189	21.45420524617541
46	21.63744411459813	28.1530454201812	29.18703267342811	21.022477791792564
47	21.333321558948214	27.320203912726292	30.014477587703226	21.33199694062227
48	21.65633219072738	27.121020564454213	30.341265834708235	20.881381410110176
49	22.200701262753743	26.456896183774603	30.24628579474401	21.09611675872764
50	20.80391576801125	27.463655171432595	30.240938261502226	21.491490799053928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1163.0
1	1867.0
2	2571.0
3	24425.0
4	46279.0
5	34906.5
6	23534.0
7	23730.0
8	23926.0
9	24361.5
10	24797.0
11	24476.5
12	24156.0
13	23568.0
14	22980.0
15	21844.0
16	20708.0
17	19814.5
18	18921.0
19	17893.0
20	16865.0
21	16770.5
22	16676.0
23	17245.5
24	17815.0
25	19351.0
26	20887.0
27	23439.5
28	25992.0
29	30771.5
30	35551.0
31	40807.5
32	46064.0
33	50771.5
34	55479.0
35	62400.0
36	69321.0
37	74124.0
38	78927.0
39	86137.5
40	93348.0
41	100949.5
42	108551.0
43	115666.0
44	122781.0
45	126129.0
46	129477.0
47	131405.0
48	133333.0
49	135289.0
50	137245.0
51	132754.5
52	128264.0
53	119502.0
54	110740.0
55	103418.0
56	96096.0
57	92628.0
58	89160.0
59	84656.5
60	80153.0
61	72404.5
62	64656.0
63	56314.5
64	47973.0
65	40586.0
66	33199.0
67	27819.5
68	22440.0
69	19971.0
70	17502.0
71	15315.5
72	13129.0
73	10480.0
74	7831.0
75	6289.0
76	4747.0
77	3634.5
78	2522.0
79	1926.5
80	1331.0
81	1032.5
82	734.0
83	512.0
84	290.0
85	205.0
86	120.0
87	89.5
88	59.0
89	40.0
90	21.0
91	13.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2038323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.899982230728753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62163210913384	24.442287027708055
2	5.953631968918851	3.798415080336199
3	2.684976208592669	2.5695208003210683
4	1.7968336252679658	2.292758428704961
5	1.3478980292438165	2.1498961590856025
6	1.071467740714112	2.050788113374755
7	0.9267703608258623	2.069477062961776
8	0.7950230102567137	2.0288975920167722
9	0.7238422268185885	2.078149875602769
>10	7.8623199230042395	49.76979844928384
>50	0.1875792505987627	3.7436543222561354
>100	0.026616531087500273	1.4754927754722331
>500	9.393436914678272E-4	0.17309808855340222
>1k	3.1311456382260907E-4	0.1047777812420767
>5k	0.0	0.0
>10k+	1.5655728191130453E-4	1.2529884430802967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25089	1.2308647844330853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010596946607578877	0.0
2	0.0	0.0	0.0	0.03919889046044223	0.0
3	0.0	0.0	0.0	0.08109607751077724	0.0
4	0.0	0.0	0.0	0.1235329238790908	0.0
5	0.0	0.0	0.0	0.1981530895741254	0.0
6	0.0	0.0	0.0	0.40734466519781215	0.0
7	0.0	0.0	0.0	0.5375006807066397	0.0
8	0.0	0.0	0.0	0.8665947447975615	0.0
9	0.0	0.0	0.0	1.068868869163523	0.0
10	0.0	0.0	0.0	1.31328548026981	0.0
11	0.0	0.0	0.0	1.4386826817928267	0.0
12	0.0	0.0	0.0	1.5524036180723075	0.0
13	0.0	0.0	0.0	1.6297711402952328	0.0
14	0.0	0.0	0.0	1.6742685040594645	0.0
15	0.0	0.0	0.0	1.706893362828168	0.0
16	0.0	0.0	0.0	1.756640139958191	0.0
17	0.0	0.0	0.0	1.8089870938021109	0.0
18	0.0	0.0	0.0	1.9003857582924786	0.0
19	0.0	0.0	0.0	1.9460115006306655	0.0
20	0.0	0.0	0.0	1.9983584544745852	0.0
21	0.0	0.0	0.0	2.047663692162626	0.0
22	0.0	0.0	0.0	2.105456299124329	0.0
23	0.0	0.0	0.0	2.176544149283504	0.0
24	0.0	0.0	0.0	2.234582055935198	0.0
25	0.0	0.0	0.0	2.2841325933132284	0.0
26	0.0	0.0	0.0	2.338147585049082	0.0
27	0.0	0.0	0.0	2.396430791390766	0.0
28	0.0	0.0	0.0	2.4590803322142762	0.0
29	0.0	0.0	0.0	2.5284510845435193	0.0
30	0.0	0.0	0.0	2.624412323267706	0.0
31	0.0	0.0	0.0	2.7007495867926723	0.0
32	0.0	0.0	0.0	2.7809625854194846	0.0
33	0.0	0.0	0.0	2.8627455020622343	0.0
34	0.0	0.0	0.0	2.943301920255033	0.0
35	0.0	0.0	0.0	3.040587777305167	0.0
36	0.0	0.0	0.0	3.1322808014235233	0.0
37	0.0	0.0	0.0	3.2239247656038814	0.0
38	0.0	0.0	0.0	3.3402458785972584	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22765	0.0	43.226883	1
TAGGGAC	1740	0.0	38.56322	5
TACGGGA	640	0.0	38.5	4
GTTTTTT	25250	0.0	38.458614	2
AGGGCGA	2030	0.0	36.955666	6
TATCCGT	30	1.3014486E-4	36.666664	44
GGGCGAT	4005	0.0	36.6392	7
GCGCGAC	965	0.0	36.476685	9
CGGTCTA	140	0.0	36.142857	31
ATAGGGA	2285	0.0	35.816193	4
CACGACG	160	0.0	35.75	26
ATAGGGC	1010	0.0	35.722775	4
ACGGGAC	505	0.0	35.722775	5
TAGGGAT	2685	0.0	35.478584	5
TAAGGGA	2385	0.0	35.421383	4
CGCGACC	1000	0.0	35.420002	10
ACGGGAT	870	0.0	35.4023	5
CCGCTGA	960	0.0	35.291664	36
CGGGATT	605	0.0	35.272728	6
CGACCCG	955	0.0	35.246075	32
>>END_MODULE