FastQCFastQC Report
Thu 26 May 2016
SRR1547173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547173_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1947559
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101060.5189059740937245No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24640.12651734812655227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG650.044.0000041
CGTTTTT66550.040.8595051
TCGATAG1650.040.01
CGTAAGG3850.038.2857172
AACGGGA5500.037.2000054
GCGTAAG2250.037.1555561
CGTATGG1850.036.8648642
GGGCGAT43250.036.8277447
CGTAGGT301.3014273E-436.66666427
ACCGTAT301.3014273E-436.66666421
ATACCCG301.3014273E-436.66666423
CGTTCGA1750.036.4571414
AGGGCGA21000.036.2476166
AGGGTAC10450.035.7894746
ACTACGG800.035.752
CGGGTAT1300.035.5384646
TAAGGGA22400.035.258934
AACCGTA250.00235314535.236
CGTAAGC250.00235314535.216
TACCGAA250.00235314535.235
Produced by FastQC (version 0.11.5)