FastQCFastQC Report
Thu 26 May 2016
SRR1547172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547172_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1540144
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92760.6022813451209758No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19040.12362480391443917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.857316E-444.00000442
ACGAACT207.857316E-444.00000444
TCGTTAG551.8189894E-1244.01
CGCATAC302.5282789E-644.021
CGTTTTT81800.042.789731
CGGTCTA1450.039.44827731
CGACGGT1650.038.66666828
TAGCACG404.125759E-738.5000041
CGGGTAT1150.038.2608686
GCGATCT2250.038.1333359
TAAGGGA15650.037.8146974
GGGCGAT30150.037.1409657
TGATTCG950.037.0526315
AAGGGAT16850.036.6884235
GTATGCG601.9826984E-1036.6666641
AACGGGA6250.036.6084
TTAGCGG4100.036.4878042
GTTTTTT96250.036.4342842
TACGGGA5150.036.310684
TAGGGTC4250.036.2352945
Produced by FastQC (version 0.11.5)