FastQCFastQC Report
Thu 26 May 2016
SRR1547171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547171_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3707501
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384741.03773404241833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT383900.043.134671
AGGGCGA41600.038.8173076
CGACGGT2250.038.13333528
GGGCGAT78550.037.838327
GTTTTTT430650.037.8339732
AAGGGCG16650.036.732735
TACGGGA15300.036.6666684
GGCGATT25150.036.652098
AGGGATT91250.036.5260286
TAGGGAT80300.036.493155
TAGGGAC44300.035.9051935
TAGGGCG17200.035.6860475
ATAGGGA68700.035.641924
AGGGATC55650.035.5795146
TGGGCGA23250.035.5784956
CGAGGGA28650.035.3228654
GAGGGAT74600.035.300275
GCGAACG751.8189894E-1235.21
GTAGGGA55450.035.1920624
AAGGGAT75050.035.1765485
Produced by FastQC (version 0.11.5)