##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547171_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3707501
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62455384368069	31.0	30.0	31.0	27.0	34.0
2	30.979238036618195	31.0	31.0	33.0	28.0	34.0
3	31.066087372599494	31.0	31.0	34.0	28.0	34.0
4	34.900248172556125	35.0	35.0	37.0	32.0	37.0
5	34.72115934695635	35.0	35.0	37.0	32.0	37.0
6	34.769083811440645	35.0	35.0	37.0	32.0	37.0
7	35.48151706499877	35.0	35.0	37.0	35.0	37.0
8	34.46121686818156	37.0	35.0	37.0	33.0	37.0
9	36.57261643354917	39.0	37.0	39.0	32.0	39.0
10	35.98292380770767	38.0	35.0	39.0	30.0	39.0
11	35.70777647801039	37.0	35.0	39.0	30.0	39.0
12	35.605193902847226	37.0	35.0	39.0	30.0	39.0
13	35.572625873870294	37.0	35.0	39.0	30.0	39.0
14	36.53978353613391	38.0	35.0	40.0	30.0	41.0
15	36.6511038028041	38.0	35.0	40.0	30.0	41.0
16	36.65554587847717	38.0	35.0	40.0	31.0	41.0
17	36.56249209373106	38.0	35.0	40.0	30.0	41.0
18	36.400256129398215	38.0	35.0	40.0	30.0	41.0
19	36.29401232798049	38.0	35.0	40.0	30.0	41.0
20	36.078407800834036	38.0	34.0	40.0	30.0	41.0
21	35.941212692862386	38.0	34.0	40.0	30.0	41.0
22	35.89424224025833	38.0	34.0	40.0	30.0	41.0
23	35.76671833669094	37.0	34.0	40.0	29.0	41.0
24	35.644786879356204	37.0	34.0	40.0	29.0	41.0
25	35.46762630677645	37.0	34.0	40.0	28.0	41.0
26	35.301965393940556	37.0	34.0	40.0	27.0	41.0
27	35.11269208019094	37.0	33.0	40.0	27.0	41.0
28	35.11457394077574	37.0	33.0	40.0	27.0	41.0
29	34.98289117116894	37.0	33.0	40.0	27.0	41.0
30	34.91199786594798	37.0	33.0	40.0	26.0	41.0
31	34.781987381797066	37.0	33.0	40.0	25.0	41.0
32	34.37686759895682	37.0	33.0	40.0	24.0	41.0
33	34.02362615681021	37.0	33.0	40.0	22.0	41.0
34	33.73390755659945	37.0	33.0	40.0	20.0	41.0
35	33.431777631347906	37.0	32.0	40.0	18.0	41.0
36	33.32259492310319	37.0	32.0	40.0	18.0	41.0
37	33.30928946479043	37.0	32.0	40.0	17.0	41.0
38	33.29736553004301	37.0	32.0	40.0	17.0	41.0
39	33.185950860161604	37.0	31.0	40.0	16.0	41.0
40	33.13662437312896	37.0	31.0	40.0	15.0	41.0
41	33.14622787694461	37.0	31.0	40.0	15.0	41.0
42	32.99418988693462	37.0	31.0	40.0	15.0	41.0
43	33.044361417569405	37.0	31.0	40.0	15.0	41.0
44	32.7524906938663	36.0	31.0	40.0	15.0	41.0
45	32.697347620405225	36.0	31.0	40.0	15.0	41.0
46	32.65559550759393	36.0	31.0	40.0	15.0	41.0
47	32.60553024800263	36.0	31.0	40.0	14.0	41.0
48	32.595320405847495	36.0	31.0	40.0	14.0	41.0
49	32.60640280339776	36.0	31.0	40.0	15.0	41.0
50	32.06928683228946	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	5.0
13	10.0
14	50.0
15	186.0
16	665.0
17	1739.0
18	3863.0
19	7372.0
20	12243.0
21	18451.0
22	26754.0
23	37166.0
24	52022.0
25	72930.0
26	98369.0
27	112938.0
28	114782.0
29	119589.0
30	133858.0
31	156330.0
32	185131.0
33	222548.0
34	286592.0
35	330801.0
36	332777.0
37	393443.0
38	483061.0
39	503821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.678427868259508	23.584592424924498	27.132912438863805	20.604067267952185
2	35.28705723882475	24.778145710547346	27.247194269131686	12.687602781496215
3	25.96870506575723	24.3308093510966	36.71553965865417	12.984945924491997
4	22.97768766616651	27.37186045263373	35.60141993218613	14.049031949013635
5	20.556083464306553	31.061003085366668	35.400098341173745	12.982815109153039
6	19.11438459490638	39.539301540309765	31.930726384160113	9.41558748062374
7	84.03282966073374	2.617666185390105	12.28868178322811	1.0608223706480457
8	82.42104317706186	4.924206358946363	11.504298987377211	1.1504514766145713
9	79.25257471272428	5.242129401988024	13.401129224240263	2.104166661047428
10	32.12274251578084	38.53641576900451	19.24414855181428	10.09669316340036
11	23.216959348089183	25.144726865886213	36.51707713632444	15.121236649700162
12	23.07090409415938	23.503459607967738	36.5005970328801	16.92503926499278
13	20.24042609833416	26.648570020614965	36.829551765461424	16.281452115589452
14	18.006900065569774	29.647598206986324	35.53647052286702	16.809031204576883
15	17.49971207020578	25.938549982859072	40.59677934004603	15.964958606889116
16	19.62729072763568	24.96662846483386	39.06955116128088	16.33652964624959
17	19.70394613514602	23.93652759635129	35.642849455738514	20.716676812764177
18	20.59144420999482	24.457444515861223	37.07928871765645	17.87182255648751
19	22.42426906965096	25.41655416950663	34.384967124756	17.77420963608641
20	23.767330069499646	25.269339104696126	33.90537183941421	17.057958986390023
21	22.230688541958585	25.593573676716474	35.30709769194937	16.868640089375567
22	21.53021671470891	24.06081616700845	34.82237766085565	19.586589457426985
23	18.778875582231805	27.529513815370514	34.504427645467935	19.18718295692975
24	18.4481676471564	26.17733616255262	37.90572140101918	17.468774789271805
25	18.681370551214957	26.911145809535856	36.3506307887712	18.056852850477988
26	18.785834447515995	28.972048827498632	34.08935021190824	18.152766513077136
27	18.614344271249017	27.608327010565876	35.327839426071634	18.449489292113476
28	17.733805061684407	27.268016920292133	37.58059674157876	17.4175812764447
29	18.500790694324827	25.333937873516422	37.27060356827955	18.8946678638792
30	19.10917893211627	26.48703264004514	36.051183802782525	18.352604625056067
31	21.15578660666578	26.646870762813013	33.80581691009659	18.39152572042462
32	21.394222145860514	27.016176125104213	33.69156205217477	17.898039676860506
33	21.24676432993545	26.833087840030252	33.005925015259606	18.914222814774696
34	19.355921953898328	27.643579866869892	33.52789385626599	19.472604322965793
35	19.67203784975378	27.684847556345904	32.87251979163323	19.77059480226708
36	21.621086548594324	27.95737614096395	31.34334960395156	19.078187706490166
37	20.36967758066687	29.493127581084945	31.548150627606038	18.589044210642154
38	20.034195540338356	29.780652790113884	30.75616163016544	19.42899003938232
39	20.453642493960217	29.610160590651226	30.50566945228066	19.4305274631079
40	21.374856001387457	28.119264162032593	30.795028780841864	19.710851055738082
41	19.54804597490331	27.871010688871024	31.177685454434133	21.403257881791536
42	20.440264210313092	28.767274776190217	29.750740458330288	21.041720555166403
43	20.583325533829928	28.551388118303944	30.307773349218248	20.557512998647876
44	20.13213212889221	29.521151848644138	30.828096877114803	19.518619145348847
45	19.20730432709256	30.15028182055784	30.12854210963126	20.513871742718344
46	20.195004667564486	29.72962111136315	30.46558854603141	19.60978567504095
47	20.44973150378112	28.654773120762478	30.646114458229412	20.249380917226993
48	20.433952681334407	27.65461155640956	31.60271568369098	20.30872007856505
49	20.230877887827948	27.62577811846848	31.68471161572175	20.458632377981825
50	19.486468108842047	29.34132721744377	31.025075920411084	20.1471287533031
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1833.0
1	2652.5
2	3472.0
3	38388.0
4	73304.0
5	56781.0
6	40258.0
7	40517.0
8	40776.0
9	41597.5
10	42419.0
11	41970.5
12	41522.0
13	40296.0
14	39070.0
15	37450.0
16	35830.0
17	34365.5
18	32901.0
19	31629.0
20	30357.0
21	30633.5
22	30910.0
23	32894.5
24	34879.0
25	40924.0
26	46969.0
27	55079.5
28	63190.0
29	73232.5
30	83275.0
31	95534.0
32	107793.0
33	120496.5
34	133200.0
35	147880.0
36	162560.0
37	174548.5
38	186537.0
39	202075.5
40	217614.0
41	236409.5
42	255205.0
43	264476.0
44	273747.0
45	282645.5
46	291544.0
47	287184.0
48	282824.0
49	272126.0
50	261428.0
51	240308.5
52	219189.0
53	198178.0
54	177167.0
55	156617.5
56	136068.0
57	123752.0
58	111436.0
59	98293.5
60	85151.0
61	74104.5
62	63058.0
63	50983.5
64	38909.0
65	31596.0
66	24283.0
67	19722.0
68	15161.0
69	11904.0
70	8647.0
71	7326.5
72	6006.0
73	4945.0
74	3884.0
75	3153.5
76	2423.0
77	2022.0
78	1621.0
79	1058.0
80	495.0
81	377.0
82	259.0
83	234.0
84	209.0
85	141.0
86	73.0
87	48.5
88	24.0
89	17.5
90	11.0
91	7.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3707501.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.65598041943095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98280831597594	20.520373640457777
2	5.680541429636167	2.914797193810236
3	2.2054412413706936	1.6974827191443602
4	1.354370026467275	1.3899076351883433
5	0.9720645592934449	1.24696346498277
6	0.7181451832843462	1.1054831256391104
7	0.5854858252560752	1.0514849008036964
8	0.508722520261647	1.0441420015005105
9	0.4350387455623121	1.0045210984056434
>10	5.972853867592864	36.60726928154918
>50	1.429047976035041	24.718143948099694
>100	0.15354556399154	5.216198766787264
>500	0.0013973160302555594	0.2435009209589689
>1k	4.299433939231702E-4	0.17875118569097576
>5k	0.0	0.0
>10k+	1.0748584848079255E-4	1.06098011698158
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38474	1.03773404241833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014322315759321441	0.0
2	0.0	0.0	0.0	0.06921103999702226	0.0
3	0.0	0.0	0.0	0.14861762680576485	0.0
4	0.0	0.0	0.0	0.22047195671693684	0.0
5	0.0	0.0	0.0	0.34014825619736855	0.0
6	0.0	0.0	0.0	0.6364664500427647	0.0
7	0.0	0.0	0.0	0.7973564943070818	0.0
8	0.0	0.0	0.0	1.1567899779393183	0.0
9	0.0	0.0	0.0	1.3765606536586235	0.0
10	0.0	0.0	0.0	1.646149252555832	0.0
11	0.0	0.0	0.0	1.7991094270777	0.0
12	0.0	0.0	0.0	1.951638044062564	0.0
13	0.0	0.0	0.0	2.054321765523462	0.0
14	0.0	0.0	0.0	2.112797811787509	0.0
15	0.0	0.0	0.0	2.1583001595953717	0.0
16	2.697234606275224E-5	0.0	0.0	2.2308287981581123	0.0
17	2.697234606275224E-5	0.0	0.0	2.3074842056684544	0.0
18	2.697234606275224E-5	0.0	0.0	2.4278617861465177	0.0
19	2.697234606275224E-5	0.0	0.0	2.4876594773676395	0.0
20	2.697234606275224E-5	0.0	0.0	2.560835452235886	0.0
21	2.697234606275224E-5	0.0	0.0	2.6331752843761875	0.0
22	2.697234606275224E-5	0.0	0.0	2.711637839072734	0.0
23	2.697234606275224E-5	0.0	0.0	2.801617585538075	0.0
24	2.697234606275224E-5	0.0	0.0	2.8768974033992167	0.0
25	2.697234606275224E-5	0.0	0.0	2.9433033194057128	0.0
26	2.697234606275224E-5	0.0	0.0	3.0105453781401543	0.0
27	2.697234606275224E-5	0.0	0.0	3.089520407411893	0.0
28	2.697234606275224E-5	0.0	0.0	3.164638391196658	0.0
29	2.697234606275224E-5	0.0	0.0	3.2445844249266553	0.0
30	2.697234606275224E-5	0.0	0.0	3.3588932275405994	0.0
31	2.697234606275224E-5	0.0	0.0	3.451273512805526	0.0
32	2.697234606275224E-5	0.0	0.0	3.5441932449917073	0.0
33	2.697234606275224E-5	0.0	0.0	3.635009134184994	0.0
34	2.697234606275224E-5	0.0	0.0	3.7309767414762667	0.0
35	2.697234606275224E-5	0.0	0.0	3.833741379975353	0.0
36	2.697234606275224E-5	0.0	0.0	3.9292504573835583	0.0
37	2.697234606275224E-5	0.0	0.0	4.032581515149962	0.0
38	2.697234606275224E-5	0.0	0.0	4.141846489050171	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38390	0.0	43.13467	1
AGGGCGA	4160	0.0	38.817307	6
CGACGGT	225	0.0	38.133335	28
GGGCGAT	7855	0.0	37.83832	7
GTTTTTT	43065	0.0	37.833973	2
AAGGGCG	1665	0.0	36.73273	5
TACGGGA	1530	0.0	36.666668	4
GGCGATT	2515	0.0	36.65209	8
AGGGATT	9125	0.0	36.526028	6
TAGGGAT	8030	0.0	36.49315	5
TAGGGAC	4430	0.0	35.905193	5
TAGGGCG	1720	0.0	35.686047	5
ATAGGGA	6870	0.0	35.64192	4
AGGGATC	5565	0.0	35.579514	6
TGGGCGA	2325	0.0	35.578495	6
CGAGGGA	2865	0.0	35.322865	4
GAGGGAT	7460	0.0	35.30027	5
GCGAACG	75	1.8189894E-12	35.2	1
GTAGGGA	5545	0.0	35.192062	4
AAGGGAT	7505	0.0	35.176548	5
>>END_MODULE