FastQCFastQC Report
Thu 26 May 2016
SRR1547170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547170_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1985533
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341441.7196390087699374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT277600.043.159941
GTTTTTT305450.038.7709922
TACGGGA9000.037.44
ACGGGAC7600.037.3421065
CGGTCTA950.037.0526331
TGGGCGA13650.036.908436
GGGCGAT40550.036.8927277
CGAATAT1850.036.86486414
TAGGGAC21450.036.8205155
TAGTACG601.9826984E-1036.6666641
CGTTAGG2650.036.52832
AACGGGA9950.036.0402034
AGGGCGA18550.035.935316
TAGGGAT37850.035.804495
AGGGATT44550.035.7037056
AAGGGAT39500.035.7012635
GTAGGGA28500.035.663164
ATAGGGA34850.035.4146354
CGGGACA6400.035.406256
AGGGATC28050.035.3725476
Produced by FastQC (version 0.11.5)