##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547170_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1985533
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.621678914427513	31.0	30.0	31.0	27.0	34.0
2	30.96355336325309	31.0	31.0	33.0	28.0	34.0
3	31.01447822826415	31.0	31.0	34.0	28.0	34.0
4	34.875919463438784	35.0	35.0	37.0	32.0	37.0
5	34.72093991890339	35.0	35.0	37.0	32.0	37.0
6	34.75710804101468	35.0	35.0	37.0	32.0	37.0
7	35.46005682101481	35.0	35.0	37.0	35.0	37.0
8	34.43096236627646	37.0	35.0	37.0	32.0	37.0
9	36.52643950012415	39.0	37.0	39.0	32.0	39.0
10	36.00485411222075	38.0	35.0	39.0	30.0	39.0
11	35.774827716285756	37.0	35.0	39.0	30.0	39.0
12	35.736455148315336	37.0	35.0	39.0	30.0	39.0
13	35.767423155394546	37.0	35.0	39.0	30.0	39.0
14	36.73599683309217	38.0	35.0	40.0	31.0	41.0
15	36.781473790664776	38.0	35.0	40.0	31.0	41.0
16	36.75170395052613	38.0	35.0	40.0	31.0	41.0
17	36.631341307346695	38.0	35.0	40.0	30.0	41.0
18	36.41622677638699	38.0	35.0	40.0	30.0	41.0
19	36.25495068578563	37.0	35.0	40.0	30.0	41.0
20	35.9918570983207	37.0	34.0	40.0	30.0	41.0
21	35.84030182323839	37.0	34.0	40.0	30.0	41.0
22	35.79506157792391	37.0	34.0	40.0	30.0	41.0
23	35.64753192215894	37.0	34.0	40.0	29.0	41.0
24	35.504161351133426	37.0	34.0	40.0	29.0	41.0
25	35.32858078913823	37.0	34.0	40.0	27.0	41.0
26	35.17184353017552	37.0	33.0	40.0	27.0	41.0
27	34.98860356387932	37.0	33.0	40.0	27.0	41.0
28	34.970536374867606	37.0	33.0	40.0	27.0	41.0
29	34.793905213360844	36.0	33.0	40.0	25.0	41.0
30	34.67776108480695	37.0	33.0	40.0	25.0	41.0
31	34.486815379044316	37.0	33.0	40.0	24.0	41.0
32	33.98360037330027	37.0	33.0	40.0	22.0	41.0
33	33.49871193276566	37.0	32.0	40.0	19.0	41.0
34	33.089380534093365	37.0	31.0	40.0	16.0	41.0
35	32.69631680762798	36.0	31.0	40.0	15.0	41.0
36	32.56773521266078	37.0	31.0	40.0	13.0	41.0
37	32.54242714676613	37.0	30.0	40.0	12.0	41.0
38	32.51211186114761	37.0	30.0	40.0	10.0	41.0
39	32.38099291223062	36.0	30.0	40.0	10.0	41.0
40	32.32253203547864	36.0	30.0	40.0	10.0	41.0
41	32.30369074701856	36.0	30.0	40.0	10.0	41.0
42	32.14422374244095	36.0	30.0	40.0	10.0	41.0
43	32.17835412456	36.0	30.0	40.0	10.0	41.0
44	31.879291354009226	36.0	30.0	40.0	10.0	41.0
45	31.82322882571078	36.0	29.0	40.0	10.0	41.0
46	31.76645918249659	36.0	29.0	40.0	10.0	41.0
47	31.70149576964976	35.0	29.0	40.0	10.0	41.0
48	31.690405044892227	35.0	29.0	39.0	10.0	41.0
49	31.689460210432163	35.0	29.0	39.0	10.0	41.0
50	31.18150693038091	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	2.0
12	3.0
13	14.0
14	25.0
15	105.0
16	394.0
17	1090.0
18	2496.0
19	4780.0
20	7844.0
21	11684.0
22	16802.0
23	23304.0
24	32664.0
25	45926.0
26	61919.0
27	70785.0
28	70638.0
29	69703.0
30	74175.0
31	83985.0
32	97391.0
33	115459.0
34	144441.0
35	166830.0
36	179111.0
37	207316.0
38	248856.0
39	247788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.949590361882677	21.21279273625772	24.67644707995284	24.161169821906764
2	39.41223842665924	22.94698703068647	25.43417812748516	12.206596415169125
3	25.50186776044518	23.78922939079834	38.427565797194	12.281337051562478
4	22.234533498058205	26.290723951704653	38.18501127908728	13.289731271149863
5	19.94189973170932	29.739621552500004	37.58028700605832	12.73819170973235
6	18.187559713185326	37.81624379952386	34.7904064047286	9.205790082562213
7	80.0533660231283	2.530605132223942	16.317633602664877	1.098395241982883
8	77.99210589801328	5.1053294002164655	15.6064895421028	1.2960751596674547
9	74.60963882242199	5.333479725595092	17.61939992938924	2.4374815225936817
10	28.69410883626714	38.11666691009417	23.073048899212452	10.116175354426241
11	21.280029090425593	24.842901125289785	38.72819036500527	15.148879419279357
12	21.861434687814306	22.51773201452708	38.864174002648156	16.756659295010458
13	20.96263320730504	23.58434737674972	39.17149702372109	16.281522392224154
14	18.66934470492306	26.035225805866734	37.929362040318644	17.366067448891556
15	18.082499762028633	24.46325495471493	40.742662045909086	16.71158323734735
16	20.815670150030243	24.18040898841772	39.037679051418436	15.966241810133603
17	20.245243972273443	23.302105782175367	37.87924955163173	18.573400693919467
18	20.887590385050263	22.830292923864775	38.59779716579881	17.684319525286156
19	22.051157044481254	24.739100281889044	36.00771178318366	17.20203089044604
20	23.620710408741633	24.173962356707243	35.79225326398504	16.41307397056609
21	22.93238138071742	24.11498574941842	36.768212867779084	16.18442000208508
22	21.769419093009283	22.56915397528019	36.95375498669627	18.707671945014262
23	19.427831217108956	25.413377667356823	37.119806117551306	18.038984997982908
24	18.981704157019802	24.490552410863984	38.8853773772584	17.642366054857813
25	19.723217896655456	25.891284607206227	36.529385308629976	17.85611218750834
26	19.484893980608735	26.14174632202033	36.231027134779424	18.142332562591506
27	18.865715150541444	24.872313882468838	37.312197782660874	18.94977318432884
28	18.294281686579875	25.648780453409742	38.231749358988246	17.825188501022144
29	19.563109754408515	24.185646876682483	37.578826441061416	18.672416927847586
30	19.892492343365735	24.423265692385872	37.71778157300836	17.966460391240034
31	20.908239752247884	25.057654544145073	36.200707820016085	17.833397883590955
32	21.371490677818	25.12106321073485	35.73891745944288	17.768528652004274
33	21.143390716749607	25.79720407568144	34.56991145450617	18.48949375306278
34	20.503814341035884	26.96248312166053	34.238212107277995	18.29549043002559
35	20.293039702689402	27.68742700322785	33.26295760382729	18.756575690255463
36	21.511201274418507	28.78587260952097	31.212777626964648	18.490148489095876
37	20.995218916029096	29.557151656507347	31.080873498451044	18.366755929012513
38	21.0392373231772	29.152978066846536	31.03977622129675	18.76800838867951
39	21.166910849630803	28.906495132541238	30.285772132722045	19.640821885105915
40	21.884300084662407	27.941414219758627	31.299353876263957	18.874931819315016
41	20.024849750671482	28.523373824559954	31.21544693540727	20.236329489361296
42	20.873589106804065	28.777512134021443	29.998846657295548	20.35005210187894
43	20.706278868193078	28.000441191357684	31.062289067973186	20.230990872476056
44	20.969936032289567	28.466915432783036	30.856450132030037	19.706698402897356
45	20.517009790318266	28.307512390879424	30.733107936256914	20.442369882545393
46	20.738411298124987	28.72875948171096	30.58181354830164	19.951015671862415
47	20.358412577378466	28.61055444558212	31.49532140740043	19.535711569638984
48	20.636876848684963	27.670403866367366	31.723471732779057	19.96924755216861
49	20.687090065992354	27.59319537877235	31.59000631064807	20.12970824458722
50	20.0169425539641	28.01101769650769	32.129810987780104	19.842228761748103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1115.0
1	1933.5
2	2752.0
3	32521.5
4	62291.0
5	46337.5
6	30384.0
7	30286.5
8	30189.0
9	30192.5
10	30196.0
11	29676.5
12	29157.0
13	28028.0
14	26899.0
15	25474.5
16	24050.0
17	22537.0
18	21024.0
19	20282.0
20	19540.0
21	18677.0
22	17814.0
23	18672.5
24	19531.0
25	20783.5
26	22036.0
27	25489.0
28	28942.0
29	33447.5
30	37953.0
31	43840.5
32	49728.0
33	55694.0
34	61660.0
35	70265.5
36	78871.0
37	83745.0
38	88619.0
39	94836.5
40	101054.0
41	106244.5
42	111435.0
43	117206.0
44	122977.0
45	126851.0
46	130725.0
47	132967.0
48	135209.0
49	134406.5
50	133604.0
51	125676.0
52	117748.0
53	108055.5
54	98363.0
55	90204.0
56	82045.0
57	75536.0
58	69027.0
59	65048.0
60	61069.0
61	53627.5
62	46186.0
63	39609.0
64	33032.0
65	26877.0
66	20722.0
67	17131.0
68	13540.0
69	11492.0
70	9444.0
71	7877.5
72	6311.0
73	5371.0
74	4431.0
75	3649.0
76	2867.0
77	2239.0
78	1611.0
79	1171.5
80	732.0
81	550.0
82	368.0
83	259.0
84	150.0
85	112.5
86	75.0
87	55.5
88	36.0
89	27.0
90	18.0
91	10.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1985533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.87439865364019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42353558820561	25.606016506628205
2	6.583475957872184	4.591895301629805
3	3.481018911108337	3.641953236805579
4	2.4580875574794816	3.4289730162036856
5	1.8987658885492777	3.3109159273600426
6	1.573587402049235	3.292674862524661
7	1.268329726074191	3.096256554396152
8	1.1550139304529745	3.2224333008899904
9	0.998519575571049	3.1340492767735526
>10	7.072970650330443	41.80357503439625
>50	0.06379203911446824	1.456035991146609
>100	0.020847666500721663	1.2078685880988795
>500	0.001761520021804589	0.39649205348973177
>1k	1.4679333515038242E-4	0.06291655697080444
>5k	0.0	0.0
>10k+	1.4679333515038242E-4	1.7479437926860428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34144	1.7196390087699374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01954135237238565	0.0
2	0.0	0.0	0.0	0.07418662898073212	0.0
3	0.0	0.0	0.0	0.15361114622622743	0.0
4	0.0	0.0	0.0	0.21087536696695547	0.0
5	0.0	0.0	0.0	0.3151294891598377	0.0
6	0.0	0.0	0.0	0.5772253596389484	0.0
7	0.0	0.0	0.0	0.7317430634494617	0.0
8	0.0	0.0	0.0	1.1122454272983626	0.0
9	0.0	0.0	0.0	1.3429643324991325	0.0
10	0.0	0.0	0.0	1.5935267759337166	0.0
11	0.0	0.0	0.0	1.7107245258577923	0.0
12	0.0	0.0	0.0	1.8220800157942476	0.0
13	0.0	0.0	0.0	1.9077497075092682	0.0
14	0.0	0.0	0.0	1.9608840548104716	0.0
15	0.0	0.0	0.0	1.998204008696909	0.0
16	0.0	0.0	0.0	2.0517412704800173	0.0
17	0.0	0.0	0.0	2.10276031675122	0.0
18	0.0	0.0	0.0	2.192811703456956	0.0
19	0.0	0.0	0.0	2.2352184526774423	0.0
20	0.0	0.0	0.0	2.2885038929093597	0.0
21	0.0	0.0	0.0	2.3394222105600866	0.0
22	0.0	0.0	0.0	2.397089345782719	0.0
23	0.0	0.0	0.0	2.459037447375591	0.0
24	0.0	0.0	0.0	2.5117185158846516	0.0
25	0.0	0.0	0.0	2.5627879264660924	0.0
26	0.0	0.0	0.0	2.6130011437734857	0.0
27	0.0	0.0	0.0	2.6810433269051686	0.0
28	0.0	0.0	0.0	2.7435454359106597	0.0
29	0.0	0.0	0.0	2.8095226823225805	0.0
30	0.0	0.0	0.0	2.906776165392366	0.0
31	0.0	0.0	0.0	2.9807109728219072	0.0
32	0.0	0.0	0.0	3.0704098093559766	0.0
33	0.0	0.0	0.0	3.151395620218853	0.0
34	0.0	0.0	0.0	3.23076977315411	0.0
35	0.0	0.0	0.0	3.3102446547098436	0.0
36	0.0	0.0	0.0	3.392691030569625	0.0
37	0.0	0.0	0.0	3.482893510206076	0.0
38	0.0	0.0	0.0	3.574707647770145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27760	0.0	43.15994	1
GTTTTTT	30545	0.0	38.770992	2
TACGGGA	900	0.0	37.4	4
ACGGGAC	760	0.0	37.342106	5
CGGTCTA	95	0.0	37.05263	31
TGGGCGA	1365	0.0	36.90843	6
GGGCGAT	4055	0.0	36.892727	7
CGAATAT	185	0.0	36.864864	14
TAGGGAC	2145	0.0	36.820515	5
TAGTACG	60	1.9826984E-10	36.666664	1
CGTTAGG	265	0.0	36.5283	2
AACGGGA	995	0.0	36.040203	4
AGGGCGA	1855	0.0	35.93531	6
TAGGGAT	3785	0.0	35.80449	5
AGGGATT	4455	0.0	35.703705	6
AAGGGAT	3950	0.0	35.701263	5
GTAGGGA	2850	0.0	35.66316	4
ATAGGGA	3485	0.0	35.414635	4
CGGGACA	640	0.0	35.40625	6
AGGGATC	2805	0.0	35.372547	6
>>END_MODULE