FastQCFastQC Report
Thu 26 May 2016
SRR1547169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547169_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3090545
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161870.5237587545238784No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46920.1518178832536009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACT302.5291101E-644.00000418
TACGCCC254.4445915E-544.029
TCGACAC254.4445915E-544.026
CGTTTTT157150.041.8720931
GCGAACG1350.039.111111
AGGGCGA35050.038.853076
ACCGTAC357.2942003E-637.71428716
TAGGGAT62200.037.0675245
ACGGGAC9400.036.744685
TCGATCT301.3016038E-436.66666817
TCCTACG301.3016038E-436.6666681
ATCCGGT601.9826984E-1036.66666835
ATCCGCG301.3016038E-436.6666681
GGGCGAT68900.036.656027
TAGTGCG2350.036.5106351
GACCGAT22200.036.4684689
GGCGATA15850.036.088338
AGGGATC43050.036.078986
GCGCGAC2200.036.09
GGGACCG25300.036.07
Produced by FastQC (version 0.11.5)