##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547169_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3090545
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.634027331748932	31.0	30.0	31.0	28.0	34.0
2	30.96752288026869	31.0	31.0	33.0	28.0	34.0
3	31.108655269539838	31.0	31.0	34.0	28.0	34.0
4	34.9266495068022	35.0	35.0	37.0	32.0	37.0
5	34.724638858194915	35.0	35.0	37.0	32.0	37.0
6	34.76583612275505	35.0	35.0	37.0	32.0	37.0
7	35.48175095331082	35.0	35.0	37.0	35.0	37.0
8	34.466844844517716	37.0	35.0	37.0	33.0	37.0
9	36.57572725846089	39.0	37.0	39.0	32.0	39.0
10	35.91725601795152	38.0	35.0	39.0	30.0	39.0
11	35.682614878605555	37.0	35.0	39.0	30.0	39.0
12	35.34173260703209	37.0	34.0	39.0	30.0	39.0
13	35.157371919839385	37.0	34.0	39.0	29.0	39.0
14	36.083863525688834	38.0	34.0	40.0	29.0	41.0
15	36.30033990768619	38.0	34.0	40.0	30.0	41.0
16	36.37636436291981	38.0	34.0	40.0	30.0	41.0
17	36.31415688818639	38.0	34.0	40.0	30.0	41.0
18	36.22914987486026	38.0	34.0	40.0	30.0	41.0
19	36.19614113368354	38.0	34.0	40.0	30.0	41.0
20	36.046528039552896	38.0	34.0	40.0	30.0	41.0
21	35.886318432509476	38.0	34.0	40.0	29.0	41.0
22	35.836432732738075	38.0	34.0	40.0	29.0	41.0
23	35.722334410273916	37.0	34.0	40.0	29.0	41.0
24	35.65168602948671	37.0	34.0	40.0	29.0	41.0
25	35.477887233481475	37.0	34.0	40.0	29.0	41.0
26	35.30083011248825	37.0	34.0	40.0	27.0	41.0
27	35.10812138312175	37.0	33.0	40.0	27.0	41.0
28	35.13693118851206	37.0	34.0	40.0	27.0	41.0
29	35.05420111986721	37.0	33.0	40.0	27.0	41.0
30	35.039151347092506	37.0	33.0	40.0	27.0	41.0
31	34.956506700274545	37.0	33.0	40.0	26.0	41.0
32	34.69252769333564	37.0	33.0	40.0	25.0	41.0
33	34.50214832658965	37.0	33.0	40.0	24.0	41.0
34	34.39563216196496	37.0	33.0	40.0	24.0	41.0
35	34.16688674651235	37.0	33.0	40.0	23.0	41.0
36	34.09814935553438	37.0	33.0	40.0	23.0	41.0
37	34.07665444120697	37.0	33.0	40.0	23.0	41.0
38	34.11656973122864	37.0	33.0	40.0	23.0	41.0
39	34.022434877990776	37.0	33.0	40.0	23.0	41.0
40	33.967567532587296	37.0	33.0	40.0	23.0	41.0
41	34.003374485729864	37.0	33.0	40.0	23.0	41.0
42	33.85187661075959	37.0	33.0	40.0	22.0	41.0
43	33.903956421925585	37.0	33.0	40.0	23.0	41.0
44	33.53963783086802	36.0	32.0	40.0	21.0	41.0
45	33.50317500635001	36.0	32.0	40.0	21.0	41.0
46	33.47301204156548	36.0	32.0	40.0	21.0	41.0
47	33.43410337011757	36.0	32.0	40.0	21.0	41.0
48	33.431056981859186	36.0	32.0	40.0	21.0	41.0
49	33.45480910324878	36.0	32.0	40.0	22.0	41.0
50	32.86256501684978	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	4.0
13	10.0
14	37.0
15	128.0
16	431.0
17	1135.0
18	2481.0
19	4539.0
20	7996.0
21	12070.0
22	17609.0
23	25150.0
24	35088.0
25	47569.0
26	62788.0
27	74391.0
28	83360.0
29	95372.0
30	113088.0
31	136403.0
32	164947.0
33	201390.0
34	272130.0
35	310634.0
36	277320.0
37	326992.0
38	401594.0
39	415884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.036629461793957	24.45296865116023	28.877107435743532	15.633294451302278
2	31.709908770136014	26.52858961768879	28.935252520186573	12.826249091988629
3	27.054742771905925	26.530239812071983	32.70241979974406	13.712597616278035
4	24.022009063126408	28.884646559102034	32.46653907320554	14.626805304566023
5	21.62430898110204	32.81686563373127	31.814129870297958	13.744695514868738
6	19.91396339480577	41.72820651373787	28.522250929852177	9.835579161604183
7	88.76508835820219	3.085669356052088	7.097971393395016	1.051270892350702
8	87.27787493791548	5.140743784672282	6.417573599478408	1.1638076779338271
9	84.0387375042266	5.573288853584077	8.319503517987927	2.068470124201395
10	36.453311632737915	36.85453536512169	15.525028757063883	11.167124245076515
11	30.734449749154276	25.385684401942054	28.9877998864278	14.892065962475872
12	28.1628644785952	24.343667540838265	30.553672572313296	16.939795408253236
13	21.071590932990784	31.71938929865121	31.667036072925647	15.54198369543236
14	18.601897076405617	34.5927012873134	31.11079113877973	15.694610497501248
15	17.600326156066327	26.79381791884603	40.38795746381302	15.217898461274629
16	19.76881100259016	24.3326015314451	38.93721010371957	16.96137736224517
17	19.72406161372832	24.143023317893768	31.28221721411596	24.85069785426195
18	21.812560567796293	24.946376771734435	33.92155752464372	19.31950513582556
19	25.06645268067606	26.311087526633653	30.15089571580417	18.471564076886114
20	26.481898823670257	26.002501176976878	29.565529704307814	17.950070295045048
21	23.285148735902567	27.6351258435001	31.20009577598773	17.879629644609608
22	22.820764622420963	25.114826025830396	30.39680703565229	21.667602316096353
23	19.431459499861674	29.64490081846406	30.332384741202606	20.591254940471664
24	19.095175769969373	26.685390440844575	36.18860103962246	18.03083274956359
25	18.841951824031035	28.04945406069156	34.18697996631662	18.92161414896078
26	18.825320453188677	31.879619937583826	30.37816954614801	18.91689006307949
27	19.05993926637535	30.554999199170375	31.60005759501965	18.78500393943463
28	17.55010847601313	28.49976298678712	35.99834333426629	17.95178520293346
29	18.419534418686673	26.51991800798888	35.034856311750836	20.025691261573606
30	19.402856130553026	29.071377378423545	32.75490245248007	18.770864038543365
31	23.694429299686625	27.721712513488722	29.598986586508204	18.98487160031645
32	24.610513679626088	27.41092590465436	30.01913901916976	17.9594213965498
33	23.647285511131532	28.622556862948123	28.863517599646666	18.866640026273686
34	20.368058060956887	28.414761797676462	30.22923141387684	20.98794872748981
35	20.663475212300746	28.045830104399062	31.302181330477314	19.988513352822885
36	24.538681688828344	27.660687678063255	28.913023431142403	18.887607201966
37	20.99966187193521	30.888338464575014	29.953163600594717	18.158836062895055
38	20.62306162828886	31.364953430543803	27.613479176002937	20.398505765164394
39	20.976882718096647	30.6321053406438	28.728719368266763	19.66229257299279
40	22.0151785526501	28.38062542367123	28.52991948022113	21.074276543457547
41	19.578035589192197	27.598206788770263	29.25335175511115	23.570405866926382
42	21.089128292906267	28.180757762789412	27.83331095324611	22.896802991058212
43	20.929641859283716	28.56486477304165	28.967123921508993	21.538369446165646
44	20.236495504838143	30.01693875999217	29.661467475801196	20.085098259368493
45	19.425376430370694	32.009758796587654	27.816420728382855	20.74844404465879
46	21.322873473772425	30.909046786246442	27.998945169864864	19.76913457011627
47	21.223214675728713	28.616020798920577	29.079207712555554	21.081556812795156
48	21.461910439744447	26.99394443374874	31.154149187279266	20.38999593922755
49	21.008074627614224	26.74612406549654	31.362720814613603	20.883080492275635
50	19.84106363117185	30.9586173312474	29.045783187107777	20.154535850472975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1971.0
1	2441.0
2	2911.0
3	16729.5
4	30548.0
5	23758.5
6	16969.0
7	17099.0
8	17229.0
9	17897.0
10	18565.0
11	18828.5
12	19092.0
13	19031.5
14	18971.0
15	18877.0
16	18783.0
17	18216.0
18	17649.0
19	17658.0
20	17667.0
21	19785.5
22	21904.0
23	24656.0
24	27408.0
25	32675.0
26	37942.0
27	42833.0
28	47724.0
29	55674.0
30	63624.0
31	73881.5
32	84139.0
33	97043.5
34	109948.0
35	121291.0
36	132634.0
37	140228.5
38	147823.0
39	166307.0
40	184791.0
41	209551.5
42	234312.0
43	254184.0
44	274056.0
45	270940.0
46	267824.0
47	254043.0
48	240262.0
49	237160.5
50	234059.0
51	222383.5
52	210708.0
53	189343.0
54	167978.0
55	144469.0
56	120960.0
57	108250.5
58	95541.0
59	83802.5
60	72064.0
61	62292.0
62	52520.0
63	43674.0
64	34828.0
65	27651.0
66	20474.0
67	15972.5
68	11471.0
69	8566.0
70	5661.0
71	4747.5
72	3834.0
73	3120.0
74	2406.0
75	1971.5
76	1537.0
77	1082.5
78	628.0
79	632.5
80	637.0
81	496.0
82	355.0
83	215.5
84	76.0
85	59.0
86	42.0
87	27.5
88	13.0
89	7.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3090545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.94737650324748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77746676662242	17.948008526088703
2	5.861565737476198	2.572919802778512
3	2.0398173167641844	1.3430591594660273
4	1.1511792621761494	1.0106145875884236
5	0.7653383868561014	0.8398584864359465
6	0.5743927555589581	0.7563848440194149
7	0.4834608793727961	0.7427488560930107
8	0.39672664963231297	0.6965687318681857
9	0.31702571517075795	0.6262094458857529
>10	4.199469366343831	22.050621134430354
>50	1.6054288164556194	25.637695931674543
>100	0.8269183609011466	24.939592135128414
>500	7.562416684834811E-4	0.09268055517363531
>1k	3.024966673933924E-4	0.20570967063431875
>5k	0.0	0.0
>10k+	1.512483336966962E-4	0.5373281327348262
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16187	0.5237587545238784	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4692	0.1518178832536009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015369457490507337	0.0
2	0.0	0.0	0.0	0.0687257425470265	0.0
3	0.0	0.0	0.0	0.12366750848151378	0.0
4	0.0	0.0	0.0	0.19242560778115186	0.0
5	0.0	0.0	0.0	0.29587014588041916	0.0
6	0.0	0.0	0.0	0.5353748287114408	0.0
7	0.0	0.0	0.0	0.6524092029075778	0.0
8	0.0	0.0	0.0	0.9593777149337738	0.0
9	0.0	0.0	0.0	1.1309979307856706	0.0
10	0.0	0.0	0.0	1.3768121803759532	0.0
11	0.0	0.0	0.0	1.5545801792240528	0.0
12	0.0	0.0	0.0	1.7216380929577146	0.0
13	0.0	0.0	0.0	1.8164110213570746	0.0
14	0.0	0.0	0.0	1.860480918414066	0.0
15	0.0	0.0	0.0	1.903159475108759	0.0
16	0.0	0.0	0.0	1.985733907773548	0.0
17	0.0	0.0	0.0	2.0784683607583774	0.0
18	0.0	0.0	0.0	2.2124900300756014	0.0
19	0.0	0.0	0.0	2.2728677304488367	0.0
20	3.23567526115944E-5	0.0	0.0	2.3439878726891212	0.0
21	3.23567526115944E-5	0.0	0.0	2.4236501976188665	0.0
22	3.23567526115944E-5	0.0	0.0	2.51625522359325	0.0
23	3.23567526115944E-5	0.0	0.0	2.620961675044369	0.0
24	3.23567526115944E-5	0.0	0.0	2.70275954564648	0.0
25	3.23567526115944E-5	0.0	0.0	2.777341860416205	0.0
26	3.23567526115944E-5	0.0	0.0	2.8496268457505067	0.0
27	3.23567526115944E-5	0.0	0.0	2.928480251864962	0.0
28	3.23567526115944E-5	0.0	0.0	3.010504619735354	0.0
29	3.23567526115944E-5	0.0	0.0	3.1023977971522823	0.0
30	3.23567526115944E-5	0.0	0.0	3.224253327487547	0.0
31	3.23567526115944E-5	0.0	0.0	3.318929185629072	0.0
32	3.23567526115944E-5	0.0	0.0	3.4118901358821825	0.0
33	3.23567526115944E-5	0.0	0.0	3.506468923765873	0.0
34	6.47135052231888E-5	0.0	0.0	3.608263267481949	0.0
35	6.47135052231888E-5	0.0	0.0	3.7235180202844482	0.0
36	6.47135052231888E-5	0.0	0.0	3.8230473913177128	0.0
37	6.47135052231888E-5	0.0	0.0	3.9338692690124235	0.0
38	6.47135052231888E-5	0.0	0.0	4.048768097536195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACT	30	2.5291101E-6	44.000004	18
TACGCCC	25	4.4445915E-5	44.0	29
TCGACAC	25	4.4445915E-5	44.0	26
CGTTTTT	15715	0.0	41.872093	1
GCGAACG	135	0.0	39.11111	1
AGGGCGA	3505	0.0	38.85307	6
ACCGTAC	35	7.2942003E-6	37.714287	16
TAGGGAT	6220	0.0	37.067524	5
ACGGGAC	940	0.0	36.74468	5
TCGATCT	30	1.3016038E-4	36.666668	17
TCCTACG	30	1.3016038E-4	36.666668	1
ATCCGGT	60	1.9826984E-10	36.666668	35
ATCCGCG	30	1.3016038E-4	36.666668	1
GGGCGAT	6890	0.0	36.65602	7
TAGTGCG	235	0.0	36.510635	1
GACCGAT	2220	0.0	36.468468	9
GGCGATA	1585	0.0	36.08833	8
AGGGATC	4305	0.0	36.07898	6
GCGCGAC	220	0.0	36.0	9
GGGACCG	2530	0.0	36.0	7
>>END_MODULE