FastQCFastQC Report
Thu 26 May 2016
SRR1547168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547168_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences395878
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.8250016419199855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.85022E-444.0000046
CGCGCGG302.523495E-644.0000042
ATTAACG302.523495E-644.0000041
CGACAGG207.85022E-444.0000042
GCCGATA207.85022E-444.0000049
CGGGATA800.044.0000046
CGACGGT254.437536E-544.028
GTTAGCG254.437536E-544.01
GCGTTGG351.4428588E-744.02
CGAATAT351.4428588E-744.014
ATAACGG254.437536E-544.02
CGTTTTT27300.042.710621
CGTAAGG650.040.6153872
GCGAAAG557.8216544E-1140.0000041
GGCCGGT452.3443135E-839.111119
CACGCCG452.3443135E-839.1111126
CGCTAGG452.3443135E-839.111112
ACGTAGG452.3443135E-839.111112
CGCGACC404.115409E-738.50000410
CGGGACG404.115409E-738.5000046
Produced by FastQC (version 0.11.5)