##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547168_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	395878
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.5920712946918	31.0	30.0	31.0	27.0	33.0
2	30.917939365157952	31.0	31.0	33.0	28.0	34.0
3	31.057590974997346	31.0	31.0	34.0	28.0	34.0
4	34.88613158599367	35.0	35.0	37.0	32.0	37.0
5	34.69130136052016	35.0	35.0	37.0	32.0	37.0
6	34.73902060735883	35.0	35.0	37.0	32.0	37.0
7	35.42421149950237	35.0	35.0	37.0	35.0	37.0
8	34.38624778340802	36.0	35.0	37.0	33.0	37.0
9	36.499843386093694	39.0	37.0	39.0	32.0	39.0
10	35.92652786969723	38.0	35.0	39.0	30.0	39.0
11	35.63048211822834	37.0	35.0	39.0	30.0	39.0
12	35.45180333335017	37.0	35.0	39.0	30.0	39.0
13	35.35729189295692	37.0	35.0	39.0	30.0	39.0
14	36.29550770691981	38.0	35.0	40.0	30.0	41.0
15	36.4291195772435	38.0	35.0	40.0	30.0	41.0
16	36.458684241104585	38.0	35.0	40.0	30.0	41.0
17	36.38044801681326	38.0	34.0	40.0	30.0	41.0
18	36.253979761441656	38.0	34.0	40.0	30.0	41.0
19	36.21940597860957	38.0	34.0	40.0	30.0	41.0
20	36.056464365284256	38.0	34.0	40.0	30.0	41.0
21	35.921086799468526	38.0	34.0	40.0	29.0	41.0
22	35.868206871814046	38.0	34.0	40.0	29.0	41.0
23	35.73352901651519	37.0	34.0	40.0	29.0	41.0
24	35.6445369533038	37.0	34.0	40.0	29.0	41.0
25	35.45251820005153	37.0	34.0	40.0	27.0	41.0
26	35.29557338371923	37.0	34.0	40.0	27.0	41.0
27	35.08371265895048	37.0	33.0	40.0	27.0	41.0
28	35.09937152355018	37.0	33.0	40.0	27.0	41.0
29	34.95953298743552	37.0	33.0	40.0	26.0	41.0
30	34.95955572171224	37.0	33.0	40.0	26.0	41.0
31	34.90087097540151	37.0	33.0	40.0	25.0	41.0
32	34.62034010477976	37.0	33.0	40.0	24.0	41.0
33	34.395424853111315	37.0	33.0	40.0	24.0	41.0
34	34.257389902949896	37.0	33.0	40.0	23.0	41.0
35	34.00308680957264	37.0	33.0	40.0	23.0	41.0
36	33.94962589484639	37.0	33.0	40.0	22.0	41.0
37	33.9508611238816	37.0	33.0	40.0	22.0	41.0
38	33.96897529036723	37.0	33.0	40.0	22.0	41.0
39	33.86005537059397	37.0	33.0	40.0	21.0	41.0
40	33.80642521180768	37.0	32.0	40.0	21.0	41.0
41	33.83057406574753	37.0	33.0	40.0	21.0	41.0
42	33.68030302264839	37.0	32.0	40.0	20.0	41.0
43	33.73463794401305	37.0	32.0	40.0	21.0	41.0
44	33.4462182793689	37.0	32.0	40.0	19.0	41.0
45	33.360833388064	36.0	31.0	40.0	19.0	41.0
46	33.324170577804274	36.0	31.0	40.0	20.0	41.0
47	33.26641035874689	36.0	31.0	40.0	19.0	41.0
48	33.267506656091015	36.0	31.0	40.0	20.0	41.0
49	33.26927992967531	36.0	31.0	40.0	20.0	41.0
50	32.70822829255478	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	15.0
16	55.0
17	155.0
18	360.0
19	691.0
20	1145.0
21	1693.0
22	2337.0
23	3478.0
24	4794.0
25	6558.0
26	8479.0
27	10109.0
28	11136.0
29	12673.0
30	14682.0
31	17314.0
32	20444.0
33	24995.0
34	32694.0
35	37389.0
36	36154.0
37	42633.0
38	52828.0
39	53064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.493288336305632	23.82526940117915	27.288710158180045	17.392732104335174
2	32.44610713401603	25.21963837343828	28.854091412000667	13.480163080545015
3	26.971693299450838	25.888783918277852	34.01123578476197	13.128286997509333
4	23.848003677900767	28.792961467926986	33.31051485558682	14.048519998585423
5	21.11307018829033	33.102117318972006	32.62368709551933	13.161125397218335
6	19.902848857476293	41.49839091841426	29.116798609672678	9.48196161443677
7	87.61133480516725	2.889779174392111	8.418755273089184	1.0801307473514568
8	85.87115222366486	5.156639166611936	7.8385260105386	1.1336825991845974
9	82.52769792713917	5.634058977765878	9.598159028791699	2.240084066303255
10	34.795063125508364	40.1045271523045	15.069289023385993	10.031120698801146
11	25.90065626278803	24.756111731391993	33.14531244474308	16.197919561076898
12	26.385401563107823	23.846740662527345	32.72775956228939	17.04009821207544
13	21.760491868707028	28.31074219835404	33.15693218617857	16.771833746760365
14	18.76664022754485	31.474848311853656	32.35870647017515	17.399804990426343
15	17.987612345217467	25.983004865135218	39.41997281990916	16.609409969738152
16	20.487624975371197	24.954405145019425	37.64897266329526	16.908997216314116
17	20.972875481840365	23.902313338957963	32.79596239245424	22.328848786747432
18	21.716286330637217	24.927124012953485	34.44874431011574	18.907845346293556
19	24.30900428920021	25.4838611895584	31.30762507641243	18.89950944482896
20	25.640475095862868	26.04514522150764	30.5227873233673	17.791592359262197
21	23.59135895402119	26.178267041866434	32.34834974411309	17.882024259999294
22	22.808794628648222	24.544935560955647	31.80954738581078	20.836722424585354
23	19.80534407064803	27.617094155270056	32.655767686004275	19.92179408807764
24	19.859148525555852	26.67564249592046	35.319972314703016	18.145236663820672
25	19.6201860169042	27.87677011604585	33.59292509308423	18.910118773965717
26	19.969536069193033	29.470442914231153	31.20380521271705	19.356215803858763
27	19.156154168708543	28.774268840400325	32.452169607808464	19.617407383082668
28	18.534245398834994	27.696158917646347	35.28182925042563	18.48776643309302
29	19.396127089658936	25.823107118859852	35.18003021132773	19.60073558015348
30	20.24840986364486	26.875451527996	33.89806960730326	18.978069001055882
31	22.757263601412557	26.842613128286995	30.984545743890795	19.415577526409653
32	23.423377909355914	27.177312202244124	31.06057926937087	18.338730619029096
33	22.34299455892977	27.646901318082843	30.634943088527272	19.375161034460113
34	20.716988567184842	27.4882665871809	31.452619241281404	20.342125604352855
35	20.103920904925253	28.147308009033082	31.826471791814647	19.92229929422701
36	22.778482259686065	28.359747194842853	29.717488721272716	19.144281824198366
37	20.68541318284926	29.91805556257231	30.484896862164607	18.911634392413827
38	20.581340716079197	30.703903727916178	28.831862341428423	19.882893214576207
39	20.600538549755225	30.585938092038457	29.14205891714114	19.671464441065176
40	21.791562046893233	28.160695971991373	29.283011432815158	20.764730548300232
41	19.32363000722445	28.115732624697507	29.82762366183521	22.733013706242833
42	20.720019804081055	28.89905475929453	28.70101394874178	21.67991148788263
43	21.036278853586207	28.385765311535373	29.537635332097263	21.04032050278116
44	20.61266349734009	29.537382729022575	29.83899080019602	20.010962973441313
45	19.936950272558718	30.52884979715973	28.85737525197157	20.676824678309984
46	21.069117253295207	30.01202390635499	29.11957724349421	19.799281596855597
47	20.488887990744622	28.8732892456767	29.88294373519114	20.75487902838753
48	21.37678779826108	27.467805738131446	30.49070673288235	20.664699730725122
49	21.06532820717494	27.52994609450386	30.817827714598945	20.586897983722256
50	20.350714108892134	29.714204881301814	29.661916044842094	20.273164964963954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	325.5
2	412.0
3	3086.0
4	5760.0
5	4286.5
6	2813.0
7	2846.5
8	2880.0
9	2973.0
10	3066.0
11	3024.5
12	2983.0
13	2903.5
14	2824.0
15	2738.0
16	2652.0
17	2468.0
18	2284.0
19	2271.0
20	2258.0
21	2457.5
22	2657.0
23	3026.0
24	3395.0
25	4004.0
26	4613.0
27	5291.5
28	5970.0
29	7201.5
30	8433.0
31	9608.5
32	10784.0
33	12338.5
34	13893.0
35	15637.5
36	17382.0
37	18607.0
38	19832.0
39	22157.0
40	24482.0
41	26377.0
42	28272.0
43	29671.0
44	31070.0
45	31775.5
46	32481.0
47	31326.0
48	30171.0
49	29057.0
50	27943.0
51	25487.0
52	23031.0
53	21719.5
54	20408.0
55	18547.5
56	16687.0
57	15099.0
58	13511.0
59	12099.0
60	10687.0
61	9318.0
62	7949.0
63	6782.5
64	5616.0
65	4499.0
66	3382.0
67	2669.0
68	1956.0
69	1587.5
70	1219.0
71	999.0
72	779.0
73	631.0
74	483.0
75	392.0
76	301.0
77	229.0
78	157.0
79	127.5
80	98.0
81	65.0
82	32.0
83	22.5
84	13.0
85	10.5
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	4.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	395878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.110643074157515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.42668210234832	28.95286190806799
2	7.624510836238143	6.268970872072649
3	4.4674602706943585	5.509804939094845
4	3.2591808313394908	5.359480794853351
5	2.617897408471026	5.381172298220714
6	2.1540971809098517	5.313379221086029
7	1.8028057631316756	5.188015298209833
8	1.5222569025632062	5.006476815075884
9	1.2343560725371723	4.567065473204511
>10	4.877396249438937	27.202845085144077
>50	0.01032084088966495	0.2600687382905141
>100	0.002428433150509399	0.17471028484007656
>500	0.0	0.0
>1k	6.071082876273498E-4	0.8151482718395572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3266	0.8250016419199855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011619741435492752	0.0
2	0.0	0.0	0.0	0.05077321801160964	0.0
3	0.0	0.0	0.0	0.09598916838015753	0.0
4	0.0	0.0	0.0	0.14474156179429015	0.0
5	0.0	0.0	0.0	0.2235537210958932	0.0
6	0.0	0.0	0.0	0.41275342403467735	0.0
7	0.0	0.0	0.0	0.5155628754313197	0.0
8	0.0	0.0	0.0	0.7820591192235992	0.0
9	0.0	0.0	0.0	0.9172017641798736	0.0
10	0.0	0.0	0.0	1.0839197934717262	0.0
11	0.0	0.0	0.0	1.1839506110468376	0.0
12	0.0	0.0	0.0	1.2945907577587035	0.0
13	0.0	0.0	0.0	1.3531946710855365	0.0
14	0.0	0.0	0.0	1.389822116914807	0.0
15	0.0	0.0	0.0	1.4219027073997546	0.0
16	0.0	0.0	0.0	1.4731811315607333	0.0
17	0.0	0.0	0.0	1.5267329833938739	0.0
18	0.0	0.0	0.0	1.6199435179525006	0.0
19	0.0	0.0	0.0	1.6651594683210484	0.0
20	0.0	0.0	0.0	1.715932686332658	0.0
21	0.0	0.0	0.0	1.7667059043442677	0.0
22	0.0	0.0	0.0	1.82909886379137	0.0
23	0.0	0.0	0.0	1.8919970293878416	0.0
24	0.0	0.0	0.0	1.9420124381753974	0.0
25	0.0	0.0	0.0	1.9884914039173684	0.0
26	0.0	0.0	0.0	2.0415380496011397	0.0
27	0.0	0.0	0.0	2.100647169077342	0.0
28	0.0	0.0	0.0	2.166576571570029	0.0
29	0.0	0.0	0.0	2.2292221340918164	0.0
30	0.0	0.0	0.0	2.31409676718585	0.0
31	0.0	0.0	0.0	2.389119880367184	0.0
32	0.0	0.0	0.0	2.4646481996978866	0.0
33	0.0	0.0	0.0	2.5389135036551664	0.0
34	0.0	0.0	0.0	2.6184834721808232	0.0
35	0.0	0.0	0.0	2.698306043781165	0.0
36	0.0	0.0	0.0	2.780654646128353	0.0
37	0.0	0.0	0.0	2.859972011579325	0.0
38	0.0	0.0	0.0	2.938026361656874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.85022E-4	44.000004	6
CGCGCGG	30	2.523495E-6	44.000004	2
ATTAACG	30	2.523495E-6	44.000004	1
CGACAGG	20	7.85022E-4	44.000004	2
GCCGATA	20	7.85022E-4	44.000004	9
CGGGATA	80	0.0	44.000004	6
CGACGGT	25	4.437536E-5	44.0	28
GTTAGCG	25	4.437536E-5	44.0	1
GCGTTGG	35	1.4428588E-7	44.0	2
CGAATAT	35	1.4428588E-7	44.0	14
ATAACGG	25	4.437536E-5	44.0	2
CGTTTTT	2730	0.0	42.71062	1
CGTAAGG	65	0.0	40.615387	2
GCGAAAG	55	7.8216544E-11	40.000004	1
GGCCGGT	45	2.3443135E-8	39.11111	9
CACGCCG	45	2.3443135E-8	39.11111	26
CGCTAGG	45	2.3443135E-8	39.11111	2
ACGTAGG	45	2.3443135E-8	39.11111	2
CGCGACC	40	4.115409E-7	38.500004	10
CGGGACG	40	4.115409E-7	38.500004	6
>>END_MODULE