FastQCFastQC Report
Thu 26 May 2016
SRR1547167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547167_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2264679
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277221.22410284194802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG408.3182385E-944.01
CGTCGAT207.858103E-444.040
CGTTTTT278850.043.3372761
CATACGA3150.039.8095218
GAGTACG950.039.368421
CGATCGA452.3526809E-839.1111110
GTTTTTT308550.038.388592
TAGTACG750.038.133331
GTTCGCC357.293338E-637.71428731
CGAGGGA14950.037.672244
CTACGCG651.0913936E-1137.230771
TGTACGG3050.036.7868842
CGTAAGG4250.036.752942
CGACGGT1200.036.66666428
AGGGATT51350.036.331066
AGGGCGA21700.036.2949336
ACGGGAT12850.035.953315
GGGCGAT44800.035.946437
TCGATAG800.035.751
GCGGGAC10100.035.722775
Produced by FastQC (version 0.11.5)