##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547167_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2264679
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.609797238372416	31.0	30.0	31.0	27.0	34.0
2	30.95190223426808	31.0	31.0	33.0	28.0	34.0
3	31.02328939333124	31.0	31.0	34.0	28.0	34.0
4	34.86888958655951	35.0	35.0	37.0	32.0	37.0
5	34.70033413123891	35.0	35.0	37.0	32.0	37.0
6	34.74364048944685	35.0	35.0	37.0	32.0	37.0
7	35.46180319594963	35.0	35.0	37.0	35.0	37.0
8	34.44580534371538	37.0	35.0	37.0	33.0	37.0
9	36.56117092091197	39.0	37.0	39.0	32.0	39.0
10	35.962024198572955	38.0	35.0	39.0	30.0	39.0
11	35.707175277379264	37.0	35.0	39.0	30.0	39.0
12	35.538345611011536	37.0	35.0	39.0	30.0	39.0
13	35.45142777409072	37.0	35.0	39.0	30.0	39.0
14	36.42329354402986	38.0	35.0	40.0	30.0	41.0
15	36.57166600652896	38.0	35.0	40.0	30.0	41.0
16	36.60109534287199	38.0	35.0	40.0	31.0	41.0
17	36.50870520722805	38.0	35.0	40.0	30.0	41.0
18	36.302119638147396	38.0	35.0	40.0	30.0	41.0
19	36.16078128511811	37.0	35.0	40.0	30.0	41.0
20	35.903891456581704	37.0	34.0	40.0	30.0	41.0
21	35.74239439673349	37.0	34.0	40.0	29.0	41.0
22	35.69915250682326	37.0	34.0	40.0	29.0	41.0
23	35.5676314391576	37.0	34.0	40.0	29.0	41.0
24	35.420868476282955	37.0	34.0	40.0	29.0	41.0
25	35.23725614093653	37.0	34.0	40.0	27.0	41.0
26	35.08430775399074	37.0	33.0	40.0	27.0	41.0
27	34.888267167223255	36.0	33.0	40.0	27.0	41.0
28	34.90103807206231	36.0	33.0	40.0	27.0	41.0
29	34.75379733728268	36.0	33.0	40.0	26.0	41.0
30	34.662321680026174	36.0	33.0	40.0	25.0	41.0
31	34.48207450150772	36.0	33.0	40.0	24.0	41.0
32	34.005206477385975	36.0	33.0	40.0	23.0	41.0
33	33.56342466194988	36.0	32.0	40.0	20.0	41.0
34	33.20961867001902	36.0	31.0	40.0	18.0	41.0
35	32.83338919113923	36.0	31.0	40.0	15.0	41.0
36	32.683706167629055	36.0	31.0	40.0	15.0	41.0
37	32.632792550290795	36.0	31.0	40.0	15.0	41.0
38	32.63554746610888	36.0	31.0	40.0	13.0	41.0
39	32.521925623896365	36.0	31.0	40.0	12.0	41.0
40	32.47999959376141	36.0	30.0	40.0	12.0	41.0
41	32.48780908905854	36.0	31.0	40.0	12.0	41.0
42	32.34360410459937	36.0	30.0	40.0	10.0	41.0
43	32.383153638992546	36.0	30.0	40.0	10.0	41.0
44	32.00867496011576	36.0	30.0	40.0	10.0	41.0
45	31.990078505607197	36.0	30.0	40.0	10.0	41.0
46	31.953001727838693	35.0	30.0	40.0	10.0	41.0
47	31.91818619769071	35.0	30.0	40.0	10.0	41.0
48	31.916329422403795	35.0	30.0	39.0	10.0	41.0
49	31.923666444560133	35.0	30.0	39.0	10.0	41.0
50	31.39428545944039	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	4.0
13	11.0
14	34.0
15	132.0
16	411.0
17	1191.0
18	2904.0
19	5464.0
20	9036.0
21	13468.0
22	19152.0
23	26728.0
24	37096.0
25	51953.0
26	69118.0
27	77283.0
28	75306.0
29	76050.0
30	83693.0
31	97074.0
32	114190.0
33	136574.0
34	177620.0
35	203752.0
36	196981.0
37	230530.0
38	279554.0
39	279369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.92819644638379	23.297385633902202	25.85854330790368	22.915874611810327
2	35.900849524369676	24.533852258973567	27.28532388033801	12.279974336318746
3	25.39604950635388	24.603663477252187	38.10743156094087	11.89285545545307
4	21.99834943495303	27.27070812243148	37.381854117073544	13.349088325541942
5	20.32981274608896	30.514169999368562	36.65451041847432	12.501506836068158
6	18.375628510707255	38.699921710759014	33.630947255659635	9.293502522874103
7	82.09229652414317	2.6168388544248433	14.234997542698105	1.0558670787338955
8	80.64670533881402	4.803506368893781	13.503768083688684	1.046020208603515
9	77.60755497799026	5.163557395992986	15.235183441008637	1.993704185008118
10	32.909078946729316	35.68995870938	21.319401116008052	10.081561227882627
11	24.50002848085755	24.637134004421817	36.28072676083454	14.582110753886091
12	24.23924096969151	22.83184504293986	36.741012743969456	16.187901243399175
13	19.944725058164973	28.142531458100684	36.65746889515027	15.255274588584077
14	17.397388327440666	30.208961181695067	35.97185296459233	16.421797526271938
15	16.77902254580009	25.45407097429702	42.46049881683011	15.306407663072779
16	18.661761777276162	24.0763922834097	41.263375515911974	15.99847042340217
17	18.816971411842474	23.18478689474314	36.52654526314767	21.471696430266718
18	20.00014130037855	24.144172308746626	37.99143278142289	17.864253609451936
19	22.657339075427466	25.384259756018402	34.55094518914159	17.407455979412532
20	23.936769846852467	24.84237280426939	34.62530451335487	16.595552835523268
21	21.8871195432112	25.22547345561998	35.92027832642065	16.967128674748167
22	21.12458321907873	23.797103253926934	35.75482441440928	19.32348911258505
23	18.57614257914698	27.191447441337164	35.568970260244384	18.663439719271473
24	18.460585363311974	25.35264379631727	39.303450952651566	16.883319887719185
25	18.249606235585706	26.925979355131567	37.14897343067163	17.675440978611096
26	18.253801090573983	29.141083570784204	34.879291943803075	17.725823394838738
27	17.887347390071618	28.014477990037438	36.36524204975628	17.732932570134665
28	17.105161482046682	26.90774277502463	38.862726240672515	17.12436950225617
29	18.081812036054558	24.979875735148337	38.528374219922554	18.409938008874548
30	18.649574619626005	26.710981997890208	36.92819158918328	17.71125179330051
31	21.17218378410362	26.438978769176558	34.29329278012469	18.095544666595135
32	21.24967821046603	26.49810414632714	34.33612445737343	17.9160931858334
33	21.51633851861566	27.553220566799975	32.76601231344486	18.164428601139498
34	19.025212844734288	27.789942857243787	33.31143177465769	19.87341252336424
35	19.753704608909253	27.620161621139243	32.8828059075922	19.7433278623593
36	22.50243853543924	27.807737873667747	30.784230347877116	18.905593243015897
37	19.827401587598064	29.709022779828842	31.980735459639092	18.482840172934
38	20.257175520239294	29.97669868444932	30.522118145662148	19.244007649649244
39	20.233816801409823	29.75640256301224	30.62853499325953	19.381245642318405
40	21.44639483123215	28.055057692503	30.678520002172494	19.820027474092356
41	19.100676078155004	27.947934343012854	31.27560241429359	21.67578716453855
42	20.313651515292015	28.949224150530828	29.170005992019178	21.567118342157983
43	20.575410466560605	28.865636145343338	30.04628912088645	20.512664267209612
44	19.861269522082377	30.23064195852922	30.370838427874325	19.537250091514075
45	19.513891372684604	30.893075795730873	29.090392059978477	20.502640771606043
46	20.33471410297	30.353308349660153	29.74359721620592	19.568380331163933
47	20.173190107737124	28.862854294140583	30.768687306236338	20.19526829188596
48	20.96747486067562	27.6512477044208	30.90190706939041	20.47937036551317
49	19.88021260408208	27.62775651648644	31.679324089639195	20.812706789792284
50	19.177331533519762	29.911391415737064	30.688499341407766	20.222777709335407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1445.0
1	1919.5
2	2394.0
3	26280.0
4	50166.0
5	38596.0
6	27026.0
7	27700.0
8	28374.0
9	28986.0
10	29598.0
11	29667.5
12	29737.0
13	29183.5
14	28630.0
15	27605.0
16	26580.0
17	25486.0
18	24392.0
19	23300.0
20	22208.0
21	22418.5
22	22629.0
23	23067.5
24	23506.0
25	25394.0
26	27282.0
27	31741.0
28	36200.0
29	41149.0
30	46098.0
31	53294.5
32	60491.0
33	67970.0
34	75449.0
35	85524.5
36	95600.0
37	102407.0
38	109214.0
39	118750.0
40	128286.0
41	140176.5
42	152067.0
43	160639.0
44	169211.0
45	169465.5
46	169720.0
47	165123.5
48	160527.0
49	160478.0
50	160429.0
51	151571.0
52	142713.0
53	125987.0
54	109261.0
55	97956.5
56	86652.0
57	76890.5
58	67129.0
59	59722.0
60	52315.0
61	45609.0
62	38903.0
63	31590.5
64	24278.0
65	19454.0
66	14630.0
67	10971.0
68	7312.0
69	6570.0
70	5828.0
71	4709.5
72	3591.0
73	2948.5
74	2306.0
75	1761.0
76	1216.0
77	945.0
78	674.0
79	499.5
80	325.0
81	240.5
82	156.0
83	118.0
84	80.0
85	48.5
86	17.0
87	17.0
88	17.0
89	13.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2264679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.306562006935202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4172831948981	24.06864817568512
2	5.881612560157582	3.565029115103693
3	2.5420715197358095	2.3112434441681193
4	1.5732401922716264	1.9071800575553082
5	1.1343235190656478	1.7188723033243998
6	0.8696815629281935	1.5814234927902966
7	0.726779072479946	1.5418322517819434
8	0.5889639843024864	1.427957880809193
9	0.494390892855299	1.3484959424984857
>10	5.938899278974209	41.56671556684814
>50	0.7824208488844315	15.264404249632973
>100	0.04884437122385105	2.110871538802168
>500	8.934013336996852E-4	0.15807826329619826
>1k	4.467006668498426E-4	0.1782498258692501
>5k	0.0	0.0
>10k+	1.4890022228328088E-4	1.2509978918347724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27722	1.22410284194802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01783917279225886	0.0
2	0.0	0.0	0.0	0.07016446922499833	0.0
3	0.0	0.0	0.0	0.12849503174622098	0.0
4	0.0	0.0	0.0	0.18302814659384398	0.0
5	0.0	0.0	0.0	0.28829692861549033	0.0
6	0.0	0.0	0.0	0.5411804498562489	0.0
7	0.0	0.0	0.0	0.6847328031919756	0.0
8	0.0	0.0	0.0	0.9893234317093063	0.0
9	0.0	0.0	0.0	1.1747801785595222	0.0
10	0.0	0.0	0.0	1.413401192840133	0.0
11	0.0	0.0	0.0	1.5521846583997114	0.0
12	0.0	0.0	0.0	1.6929551605326847	0.0
13	4.415636829767044E-5	0.0	0.0	1.7798990497107978	0.0
14	4.415636829767044E-5	0.0	0.0	1.8254684217939938	0.0
15	4.415636829767044E-5	0.0	0.0	1.8642377131593484	0.0
16	4.415636829767044E-5	0.0	0.0	1.927116381615231	0.0
17	4.415636829767044E-5	0.0	0.0	1.9952496578985366	0.0
18	4.415636829767044E-5	0.0	0.0	2.107097738796536	0.0
19	4.415636829767044E-5	0.0	0.0	2.161851635485647	0.0
20	4.415636829767044E-5	0.0	0.0	2.222036765475372	0.0
21	4.415636829767044E-5	0.0	0.0	2.2895076962342125	0.0
22	4.415636829767044E-5	0.0	0.0	2.36196829661069	0.0
23	4.415636829767044E-5	0.0	0.0	2.444849799905417	0.0
24	4.415636829767044E-5	0.0	0.0	2.516780523862322	0.0
25	4.415636829767044E-5	0.0	0.0	2.581911167101386	0.0
26	4.415636829767044E-5	0.0	0.0	2.6468210284989615	0.0
27	4.415636829767044E-5	0.0	0.0	2.720473850819476	0.0
28	4.415636829767044E-5	0.0	0.0	2.7890045344174603	0.0
29	4.415636829767044E-5	0.0	0.0	2.875462703544299	0.0
30	4.415636829767044E-5	0.0	0.0	2.991108232115898	0.0
31	4.415636829767044E-5	0.0	0.0	3.0817612562310157	0.0
32	4.415636829767044E-5	0.0	0.0	3.1707363383508214	0.0
33	4.415636829767044E-5	0.0	0.0	3.265760842927408	0.0
34	4.415636829767044E-5	0.0	0.0	3.3585775290891116	0.0
35	4.415636829767044E-5	0.0	0.0	3.4587683287565256	0.0
36	4.415636829767044E-5	0.0	0.0	3.558252626531177	0.0
37	4.415636829767044E-5	0.0	0.0	3.6577810806741264	0.0
38	4.415636829767044E-5	0.0	0.0	3.770909696252758	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	8.3182385E-9	44.0	1
CGTCGAT	20	7.858103E-4	44.0	40
CGTTTTT	27885	0.0	43.337276	1
CATACGA	315	0.0	39.80952	18
GAGTACG	95	0.0	39.36842	1
CGATCGA	45	2.3526809E-8	39.11111	10
GTTTTTT	30855	0.0	38.38859	2
TAGTACG	75	0.0	38.13333	1
GTTCGCC	35	7.293338E-6	37.714287	31
CGAGGGA	1495	0.0	37.67224	4
CTACGCG	65	1.0913936E-11	37.23077	1
TGTACGG	305	0.0	36.786884	2
CGTAAGG	425	0.0	36.75294	2
CGACGGT	120	0.0	36.666664	28
AGGGATT	5135	0.0	36.33106	6
AGGGCGA	2170	0.0	36.294933	6
ACGGGAT	1285	0.0	35.95331	5
GGGCGAT	4480	0.0	35.94643	7
TCGATAG	80	0.0	35.75	1
GCGGGAC	1010	0.0	35.72277	5
>>END_MODULE