FastQCFastQC Report
Thu 26 May 2016
SRR1547166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547166_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3458779
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146020.42217210177348713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA57200.16537627873882663No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA42750.12359853000148317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1650.044.01
CGTTTTT115600.040.993081
CGTTAGG4650.037.849462
CGTAAGG5850.037.606842
TACGAAT5400.037.4814812
GGGCGAT73800.037.441747
TAGGGAC30700.037.3355035
GGCGATA16450.036.9118548
GACCGAT26100.036.7509579
CGAATAT5450.036.73394414
CGCATGG3500.036.457142
ATATGCG1450.036.413791
CATATGC28850.036.29809633
TATAGCG2850.036.2807041
GGTACCT29250.036.0273488
TCACGAC4100.035.95121825
AGGGCGA40250.035.9105576
CGGTCTA4150.035.51807431
TATACTA22750.035.4901144
GGACCGA27550.035.4555368
Produced by FastQC (version 0.11.5)