##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547166_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3458779
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.584074033061956	31.0	30.0	31.0	28.0	33.0
2	30.911350219253674	31.0	31.0	33.0	28.0	34.0
3	31.09023097457224	31.0	31.0	34.0	28.0	34.0
4	34.91927613761966	35.0	35.0	37.0	32.0	37.0
5	34.709544900093356	35.0	35.0	37.0	32.0	37.0
6	34.74164033030153	35.0	35.0	37.0	32.0	37.0
7	35.3647729444408	35.0	35.0	37.0	33.0	37.0
8	34.33030442245659	35.0	35.0	37.0	32.0	37.0
9	36.43339629389446	39.0	37.0	39.0	32.0	39.0
10	35.863783144283	37.0	35.0	39.0	30.0	39.0
11	35.62154448144851	37.0	35.0	39.0	30.0	39.0
12	35.08074323337802	37.0	34.0	39.0	30.0	39.0
13	34.788891397802516	37.0	33.0	39.0	27.0	39.0
14	35.64889864313389	38.0	33.0	40.0	27.0	41.0
15	35.91227655771011	38.0	34.0	40.0	29.0	41.0
16	36.05545829901246	38.0	34.0	40.0	30.0	41.0
17	35.97504784202749	38.0	34.0	40.0	30.0	41.0
18	35.92576975863448	38.0	34.0	40.0	30.0	41.0
19	35.88880295618772	37.0	34.0	40.0	29.0	41.0
20	35.74100571328784	37.0	34.0	40.0	29.0	41.0
21	35.55222348695884	37.0	34.0	40.0	29.0	41.0
22	35.49485902395036	37.0	34.0	40.0	29.0	41.0
23	35.38591682209242	37.0	34.0	40.0	28.0	41.0
24	35.29499369575217	37.0	34.0	40.0	27.0	41.0
25	35.08693703760778	37.0	33.0	40.0	27.0	41.0
26	34.884987737001985	36.0	33.0	40.0	27.0	41.0
27	34.684240305610736	36.0	33.0	40.0	26.0	41.0
28	34.69453295512665	36.0	33.0	40.0	26.0	41.0
29	34.5163186199523	36.0	33.0	40.0	25.0	41.0
30	34.53119641353206	36.0	33.0	40.0	25.0	41.0
31	34.465343984105374	36.0	33.0	40.0	25.0	41.0
32	34.24004511418625	36.0	33.0	40.0	24.0	41.0
33	34.12250305671452	36.0	33.0	40.0	24.0	41.0
34	34.002292716591604	36.0	33.0	40.0	23.0	41.0
35	33.81712853003907	36.0	32.0	40.0	23.0	41.0
36	33.70418491612214	36.0	32.0	40.0	23.0	41.0
37	33.699288101379125	36.0	32.0	40.0	23.0	41.0
38	33.68614300017434	36.0	32.0	40.0	23.0	41.0
39	33.58292622916931	35.0	32.0	40.0	23.0	41.0
40	33.4599478602131	35.0	32.0	39.0	22.0	41.0
41	33.4726890616602	35.0	32.0	39.0	22.0	41.0
42	33.30526957634471	35.0	31.0	39.0	22.0	41.0
43	33.32988028434312	35.0	32.0	39.0	22.0	41.0
44	33.017759157205475	35.0	31.0	39.0	21.0	41.0
45	32.93006173565874	35.0	31.0	39.0	21.0	41.0
46	32.87219449406857	35.0	31.0	39.0	21.0	40.0
47	32.77503217175772	35.0	31.0	39.0	20.0	40.0
48	32.772601834346744	35.0	31.0	39.0	21.0	40.0
49	32.770286277325035	35.0	31.0	39.0	21.0	40.0
50	32.173868003708826	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	7.0
12	10.0
13	18.0
14	67.0
15	216.0
16	617.0
17	1373.0
18	3119.0
19	5785.0
20	9775.0
21	15417.0
22	22662.0
23	31243.0
24	42559.0
25	56985.0
26	72805.0
27	88319.0
28	102595.0
29	118643.0
30	141242.0
31	167443.0
32	200621.0
33	245390.0
34	333563.0
35	368182.0
36	311929.0
37	356931.0
38	401449.0
39	359806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.1460032572188	24.46447142185147	27.946769654840626	14.442755666089102
2	30.709681075315885	26.196065143219617	29.509806784417275	13.584446997047223
3	28.938680384031475	26.28676188909439	30.831024474243655	13.94353325263048
4	25.60380411700198	29.765272658357183	29.438105181047998	15.19281804359284
5	22.58577376582892	32.85780907077324	29.759808302293962	14.796608861103874
6	21.46566750867864	41.52786286721412	26.44895785478055	10.55751176932669
7	88.38373310350272	3.916468788552261	6.18524629645317	1.5145518114918588
8	86.49806767070115	5.9154690137762485	5.6823520670155565	1.9041112485070597
9	83.71682608226776	5.27929075549493	7.968910416074575	3.0349727461627354
10	43.89355318741093	29.020587901106143	14.127442082885318	12.958416828597608
11	38.430382513598005	24.073842243173097	21.780981091882424	15.714794151346473
12	33.80519541722671	21.60950439446984	26.106293579323804	18.479006608979642
13	24.14450301681605	30.646537405251966	27.318253059822556	17.890706518109425
14	20.582465662015412	32.66846479639202	28.631144111838307	18.11792542975426
15	19.21420825094636	22.850317987937363	39.58278340420131	18.35269035691497
16	22.434159569027106	19.898495972133517	36.850749932273786	20.816594526565588
17	21.8695672663677	20.776146726923	28.62310659339611	28.731179413313196
18	24.68110856461196	21.948785973316017	31.811717371939636	21.558388090132386
19	29.100789613907104	24.466177226125176	24.967076531920657	21.465956628047064
20	30.18961315539385	24.170639407721627	24.605330378147897	21.034417058736622
21	26.122021672966095	26.46610263332812	27.254126383905998	20.15774930979979
22	25.807459800120213	22.485767376290884	26.236455118988523	25.470317704600383
23	22.659846148019287	27.24741881455855	25.510794416179817	24.581940621242353
24	23.14799528966725	23.700531314663355	32.99193154578538	20.159541849884018
25	22.547783480817944	24.664715496422293	30.19770849770974	22.589792525050026
26	21.121904579621884	28.784637584534888	26.49009954090735	23.603358294935873
27	21.43406676170984	27.624575030668336	27.662305108247736	23.279053099374085
28	20.517037948940942	26.343082342063482	31.883447887245758	21.256431821749814
29	21.424699294172886	24.77680707556048	31.228216662585268	22.57027696768137
30	22.989297668339027	26.719139904573264	29.155172967107756	21.136389459979952
31	28.09890426650561	25.254085328955682	25.23474324320808	21.412267161330632
32	28.210562166591156	24.72317543271773	25.563963468033084	21.50229893265803
33	27.474579902329694	24.75133565920228	24.862097289245714	22.911987149222313
34	21.85274051912539	26.378730760190226	27.583404432604684	24.185124288079695
35	22.978368956212584	25.048752753500587	28.847289751672484	23.125588538614352
36	28.17144431604332	25.219304268934213	24.71652568724397	21.892725727778505
37	23.20032589535209	29.042040558243244	26.494262859812668	21.263370686592005
38	24.083816861383742	29.10833562942298	24.58723150568452	22.220616003508752
39	23.294492073647955	27.605232944920736	25.575557154706907	23.5247178267244
40	25.05294498434274	24.883925801561766	26.301333505262985	23.76179570883251
41	21.639052393922825	23.470276649650064	27.294805479043326	27.59586547738378
42	23.252656501036927	25.392602418367872	24.89505689724611	26.459684183349097
43	22.219112582792945	24.99714494623681	27.362228115759923	25.421514355210324
44	22.31949482750994	25.916457802016264	28.42413464404635	23.339912726427446
45	21.021493422968046	29.224041200666477	25.77051034483556	23.983955031529913
46	23.220766634699704	27.74427044919609	26.371849719221725	22.663113196882485
47	23.049116465666064	26.40021232926417	27.057004798514157	23.493666406555608
48	23.422311746428438	24.875194396635344	28.80114630047193	22.90134755646429
49	23.090055768234976	23.998121880582715	29.445044045890185	23.466778305292127
50	22.390271248900262	28.08028497917907	26.836753663648356	22.692690108272313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2282.0
1	3298.0
2	4314.0
3	15248.0
4	26182.0
5	19387.0
6	12592.0
7	12986.5
8	13381.0
9	14363.0
10	15345.0
11	15461.0
12	15577.0
13	15709.0
14	15841.0
15	15355.5
16	14870.0
17	14911.0
18	14952.0
19	14089.0
20	13226.0
21	14080.0
22	14934.0
23	16338.0
24	17742.0
25	19215.0
26	20688.0
27	24939.0
28	29190.0
29	34872.5
30	40555.0
31	45367.5
32	50180.0
33	61178.5
34	72177.0
35	79658.0
36	87139.0
37	94289.5
38	101440.0
39	119012.0
40	136584.0
41	167745.5
42	198907.0
43	222312.0
44	245717.0
45	256339.5
46	266962.0
47	265662.0
48	264362.0
49	260834.5
50	257307.0
51	242510.0
52	227713.0
53	215209.0
54	202705.0
55	193380.5
56	184056.0
57	180515.5
58	176975.0
59	171222.5
60	165470.0
61	154739.0
62	144008.0
63	133820.5
64	123633.0
65	106440.5
66	89248.0
67	75720.0
68	62192.0
69	55032.5
70	47873.0
71	39956.5
72	32040.0
73	26888.5
74	21737.0
75	17051.5
76	12366.0
77	10028.5
78	7691.0
79	6221.0
80	4751.0
81	3355.0
82	1959.0
83	1472.0
84	985.0
85	796.0
86	607.0
87	387.0
88	167.0
89	134.0
90	101.0
91	69.0
92	37.0
93	25.0
94	13.0
95	9.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3458779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.745179185458102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.35156590298186	17.083979908604206
2	6.305256066590445	2.6160733382323107
3	1.9613381583741318	1.220649346162433
4	1.0085209699109574	0.8368779293237925
5	0.6646823919607251	0.6894477661322069
6	0.47996346338157453	0.5974156830194294
7	0.38971125022595704	0.5659240801568465
8	0.3090128383871471	0.5128421362358698
9	0.27277442808763563	0.5092878949099972
>10	3.5139518694675935	18.008449242849153
>50	1.6962139345999983	25.7024661136853
>100	1.0408604420309902	30.051626844234374
>500	0.004861433861305885	0.6590633536610729
>1k	0.0010008834420335646	0.3431021113370372
>5k	1.4298334886193777E-4	0.16966750902116826
>10k+	1.4298334886193777E-4	0.4331267424348075
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14602	0.42217210177348713	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5720	0.16537627873882663	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	4275	0.12359853000148317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018012136652847725	0.0
2	0.0	0.0	0.0	0.07820678915883322	0.0
3	0.0	0.0	0.0	0.12952547705418588	0.0
4	0.0	0.0	0.0	0.21906574545526036	0.0
5	0.0	0.0	0.0	0.3522919504252801	0.0
6	0.0	0.0	0.0	0.6724627390185959	0.0
7	0.0	0.0	0.0	0.8277198398625643	0.0
8	0.0	0.0	0.0	1.2634516400151614	0.0
9	0.0	0.0	0.0	1.4927522111126499	0.0
10	0.0	0.0	0.0	1.8079501465690637	0.0
11	0.0	0.0	0.0	2.0039152544872048	0.0
12	0.0	0.0	0.0	2.185453305920962	0.0
13	0.0	0.0	0.0	2.273605801353599	0.0
14	0.0	0.0	0.0	2.31700261855412	0.0
15	0.0	0.0	0.0	2.3575660659440802	0.0
16	0.0	0.0	0.0	2.43478984925027	0.0
17	0.0	0.0	0.0	2.519415088388128	0.0
18	0.0	0.0	0.0	2.658828447842432	0.0
19	0.0	0.0	0.0	2.713963511400989	0.0
20	0.0	0.0	0.0	2.785983146075537	0.0
21	0.0	0.0	0.0	2.8655487962659656	0.0
22	0.0	0.0	0.0	2.9476297849616873	0.0
23	0.0	0.0	0.0	3.0441089182049503	0.0
24	0.0	0.0	0.0	3.1206099030900787	0.0
25	0.0	0.0	0.0	3.1858352326066512	0.0
26	2.8911936842452205E-5	0.0	0.0	3.25160988892323	0.0
27	2.8911936842452205E-5	0.0	0.0	3.32094071347143	0.0
28	2.8911936842452205E-5	0.0	0.0	3.388710293430138	0.0
29	2.8911936842452205E-5	0.0	0.0	3.4695480688416347	0.0
30	2.8911936842452205E-5	0.0	0.0	3.5766378829060774	0.0
31	2.8911936842452205E-5	0.0	0.0	3.6627376308229	0.0
32	2.8911936842452205E-5	0.0	0.0	3.7529717857081937	0.0
33	2.8911936842452205E-5	0.0	0.0	3.8390137097513315	0.0
34	2.8911936842452205E-5	0.0	0.0	3.92731076486818	0.0
35	2.8911936842452205E-5	0.0	0.0	4.032290007543124	0.0
36	2.8911936842452205E-5	0.0	0.0	4.119690792617857	0.0
37	2.8911936842452205E-5	0.0	0.0	4.213770235103197	0.0
38	2.8911936842452205E-5	0.0	0.0	4.32334647573609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	165	0.0	44.0	1
CGTTTTT	11560	0.0	40.99308	1
CGTTAGG	465	0.0	37.84946	2
CGTAAGG	585	0.0	37.60684	2
TACGAAT	540	0.0	37.48148	12
GGGCGAT	7380	0.0	37.44174	7
TAGGGAC	3070	0.0	37.335503	5
GGCGATA	1645	0.0	36.911854	8
GACCGAT	2610	0.0	36.750957	9
CGAATAT	545	0.0	36.733944	14
CGCATGG	350	0.0	36.45714	2
ATATGCG	145	0.0	36.41379	1
CATATGC	2885	0.0	36.298096	33
TATAGCG	285	0.0	36.280704	1
GGTACCT	2925	0.0	36.027348	8
TCACGAC	410	0.0	35.951218	25
AGGGCGA	4025	0.0	35.910557	6
CGGTCTA	415	0.0	35.518074	31
TATACTA	2275	0.0	35.49011	44
GGACCGA	2755	0.0	35.455536	8
>>END_MODULE