FastQCFastQC Report
Thu 26 May 2016
SRR1547165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547165_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2578823
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304221.1796854611580554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT291150.043.176371
GCGTAAG1750.038.9714241
GTTTTTT321000.038.530842
TATCGCG750.038.133331
CGTTAGG2850.037.824562
TCGTTAG357.293729E-637.7142871
CGTAAGG3850.037.7142832
AGGGCGA26350.036.736246
AAGGGAT51850.036.2352945
TAACGGG7700.035.9999963
GCGCGAC3000.035.9333349
TCGATAG1350.035.8518521
AGGGATT60350.035.761396
GGGCGAT52600.035.7604567
TAGGGAT51500.035.5417445
TACGTAG1550.035.4838681
ACGGGAT14450.035.474055
ATAGGGA40200.035.243784
CGACGGA250.002353285235.23
GCGAACG1000.035.21
Produced by FastQC (version 0.11.5)