##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547165_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2578823
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.603002610105463	31.0	30.0	31.0	27.0	34.0
2	30.95571623178481	31.0	31.0	33.0	28.0	34.0
3	31.035888465396813	31.0	31.0	34.0	28.0	34.0
4	34.88045127564009	35.0	35.0	37.0	32.0	37.0
5	34.69358540698605	35.0	35.0	37.0	32.0	37.0
6	34.73937257423251	35.0	35.0	37.0	32.0	37.0
7	35.45891904950437	35.0	35.0	37.0	35.0	37.0
8	34.43968857110395	37.0	35.0	37.0	33.0	37.0
9	36.54728261691477	39.0	37.0	39.0	32.0	39.0
10	35.95796376874256	38.0	35.0	39.0	30.0	39.0
11	35.679481298251176	37.0	35.0	39.0	30.0	39.0
12	35.52212268930438	37.0	35.0	39.0	30.0	39.0
13	35.46070668673267	37.0	35.0	39.0	30.0	39.0
14	36.41968293287287	38.0	35.0	40.0	30.0	41.0
15	36.543789938278046	38.0	35.0	40.0	30.0	41.0
16	36.555298676954564	38.0	35.0	40.0	30.0	41.0
17	36.47126576736752	38.0	35.0	40.0	30.0	41.0
18	36.287669607413925	38.0	35.0	40.0	30.0	41.0
19	36.16846871615462	37.0	34.0	40.0	30.0	41.0
20	35.9287993010765	37.0	34.0	40.0	30.0	41.0
21	35.76938006214463	37.0	34.0	40.0	29.0	41.0
22	35.736689567294846	37.0	34.0	40.0	29.0	41.0
23	35.605852747551886	37.0	34.0	40.0	29.0	41.0
24	35.47746200495342	37.0	34.0	40.0	29.0	41.0
25	35.304572279679526	37.0	34.0	40.0	27.0	41.0
26	35.15348436088867	37.0	34.0	40.0	27.0	41.0
27	34.9522444929334	37.0	33.0	40.0	27.0	41.0
28	34.964066165068324	37.0	33.0	40.0	27.0	41.0
29	34.82802735976839	36.0	33.0	40.0	26.0	41.0
30	34.74953302339866	37.0	33.0	40.0	25.0	41.0
31	34.59186962424331	37.0	33.0	40.0	25.0	41.0
32	34.145737028093826	37.0	33.0	40.0	23.0	41.0
33	33.75275619924283	37.0	32.0	40.0	21.0	41.0
34	33.43342563642406	37.0	32.0	40.0	18.0	41.0
35	33.08642237175642	37.0	31.0	40.0	17.0	41.0
36	32.96399326359351	37.0	31.0	40.0	15.0	41.0
37	32.9349431891991	37.0	31.0	40.0	15.0	41.0
38	32.92305869770822	37.0	31.0	40.0	15.0	41.0
39	32.81061747936946	37.0	31.0	40.0	15.0	41.0
40	32.752366098797786	36.0	31.0	40.0	15.0	41.0
41	32.76386048984362	36.0	31.0	40.0	15.0	41.0
42	32.612676403149806	36.0	31.0	40.0	14.0	41.0
43	32.670894435174496	36.0	31.0	40.0	14.0	41.0
44	32.38906198680561	36.0	30.0	40.0	12.0	41.0
45	32.3292083248831	36.0	30.0	40.0	12.0	41.0
46	32.27563698633059	36.0	30.0	40.0	12.0	41.0
47	32.22592399711031	36.0	30.0	40.0	11.0	41.0
48	32.21914338440443	36.0	30.0	40.0	11.0	41.0
49	32.230602875808074	36.0	30.0	40.0	11.0	41.0
50	31.69158565748793	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	8.0
14	50.0
15	153.0
16	509.0
17	1420.0
18	3229.0
19	5965.0
20	9607.0
21	14456.0
22	20800.0
23	28715.0
24	39668.0
25	55420.0
26	73961.0
27	84318.0
28	84768.0
29	86519.0
30	95318.0
31	109536.0
32	129754.0
33	155459.0
34	200881.0
35	229165.0
36	225115.0
37	264504.0
38	324682.0
39	334836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.572522038154613	22.85484502038333	26.193771344524226	21.378861596937828
2	36.246458171033844	24.397370428292287	26.901807530024357	12.454363870649516
3	25.639332362089217	24.703789286818058	37.17909294278824	12.477785408304486
4	22.60888009762593	27.186123281822756	36.522747005125986	13.682249615425333
5	20.542898834080507	30.729832950923736	36.02263513238404	12.70463308261172
6	18.709892070917626	38.90185561397583	32.993966627411034	9.394285687695511
7	82.88164794559378	2.813647931633928	13.31669525205879	0.9880088707134999
8	81.3908127855227	4.921741430102027	12.555844274694309	1.1316015096809668
9	78.16930436869843	5.215441307914502	14.38714483312736	2.228109490259704
10	33.27242699479569	36.22831035708926	20.373635569405113	10.125627078709938
11	24.432231293113176	24.2517613655532	36.07599280757152	15.240014533762109
12	23.85309111947582	22.966174879004882	36.63520140777401	16.545532593745286
13	20.623051679002398	26.93655206270458	36.85022198111309	15.590174277179939
14	17.988322579719508	29.268468599822477	36.06695767797945	16.676251142478566
15	17.152398594242413	25.14286556308828	41.89612858269063	15.808607259978679
16	19.52685391746545	24.188088907226284	40.40277289290502	15.88228428240325
17	19.71531198535146	23.405987925499346	36.12504619355419	20.753653895595008
18	20.74582086478987	23.81268508928298	37.80573540719933	17.635758638727822
19	23.160449553924405	25.041928042366614	34.52268728796044	17.27493511574854
20	24.3517682291495	25.068063996637225	33.99841710733928	16.581750666873994
21	22.244372723525423	25.52075113336588	35.56479060408566	16.670085539023034
22	21.32391404916119	23.610848825219875	35.578866793106776	19.486370332512156
23	18.812729683270234	27.395404802888763	35.310488544580224	18.481376969260783
24	18.61163018943138	25.25376887052737	38.67601615155441	17.458584788486842
25	18.66646140506735	26.332788252625328	37.10153818234133	17.899212159965998
26	18.74238751554488	28.420950177658565	34.599931829365566	18.23673047743098
27	18.24801469507601	27.587430389755323	35.96536094179399	18.199193973374676
28	17.689504087717538	26.69283622800014	38.36723187283501	17.250427811447317
29	18.457955431605814	24.90104982001479	38.144494600831464	18.49650014754793
30	18.873416283319948	26.125368045810042	36.78561886566081	18.2155968052092
31	21.438346098200615	25.720881192699153	34.657748903278744	18.183023805821495
32	21.907397289383567	26.09826265703385	34.46409466644279	17.530245387139793
33	21.84969654761106	26.59589277744149	33.42059536462953	18.133815310317924
34	19.421495775398313	27.468267500328636	33.78611095061584	19.324125773657208
35	19.75110350729771	27.615156216615098	33.37468294644495	19.259057329642243
36	22.29412410235212	28.206278600741502	30.88447714325489	18.61512015365149
37	20.613085892284968	30.09291448075343	30.936206168472978	18.35779345848862
38	20.694906164556468	30.471149047453043	29.9338496670768	18.900095120913686
39	20.801776624452316	29.704481463055043	30.43714128499707	19.05660062749557
40	21.915113988048034	28.06982099973515	30.28168276768122	19.733382244535587
41	19.691541451274478	27.80229585357351	30.608847524626544	21.89731517052547
42	20.42990154810935	28.528906404200676	29.668224612546112	21.37296743514386
43	20.431840417120522	28.43498758929946	30.396076039340425	20.73709595423959
44	20.017659218953764	29.50776381318144	30.97579787368113	19.498779094183664
45	19.604757674334376	30.33116270484636	29.67314933983449	20.390930280984776
46	20.59400742121503	30.099622967532085	29.816625646661286	19.4897439645916
47	20.5318085033366	28.55046662760492	30.436753511194837	20.480971357863645
48	20.8701799231665	27.410450426415462	31.54330483325145	20.17606481716659
49	20.627588632488543	27.10759908687025	31.788028879841697	20.476783400799512
50	19.709766819979503	29.340400640136995	30.69144334450251	20.258389195380992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1429.0
1	2114.0
2	2799.0
3	30147.5
4	57496.0
5	43860.5
6	30225.0
7	30329.0
8	30433.0
9	31076.0
10	31719.0
11	31420.5
12	31122.0
13	30527.0
14	29932.0
15	28426.0
16	26920.0
17	25374.0
18	23828.0
19	23245.5
20	22663.0
21	22467.0
22	22271.0
23	24104.5
24	25938.0
25	28805.0
26	31672.0
27	36801.5
28	41931.0
29	48185.0
30	54439.0
31	62228.0
32	70017.0
33	78065.0
34	86113.0
35	95585.5
36	105058.0
37	113518.0
38	121978.0
39	134321.5
40	146665.0
41	157728.5
42	168792.0
43	182176.0
44	195560.0
45	195287.5
46	195015.0
47	193628.5
48	192242.0
49	186349.0
50	180456.0
51	167560.5
52	154665.0
53	139458.5
54	124252.0
55	112700.0
56	101148.0
57	91050.0
58	80952.0
59	72547.0
60	64142.0
61	57654.5
62	51167.0
63	41365.0
64	31563.0
65	24308.5
66	17054.0
67	13869.5
68	10685.0
69	8561.5
70	6438.0
71	5310.5
72	4183.0
73	3312.5
74	2442.0
75	2054.5
76	1667.0
77	1394.0
78	1121.0
79	749.5
80	378.0
81	272.5
82	167.0
83	105.0
84	43.0
85	34.0
86	25.0
87	15.5
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2578823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.65190341306649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54642963423116	22.505047153212146
2	5.554803816496405	3.1831140485757623
3	2.3294390724895546	2.0022858993478176
4	1.4262037052142347	1.6345380323662322
5	1.1549447822651815	1.6545683174443544
6	0.8991274411695677	1.5457027560256842
7	0.7259611998549724	1.456011912591497
8	0.651440026553171	1.493199737616554
9	0.5711674705464606	1.4728531678904417
>10	7.49569364112007	49.099137613851966
>50	0.6052099759249837	10.81860134237256
>100	0.03833425230331888	1.6436899006437873
>500	8.299878872573949E-4	0.1448246626284582
>1k	2.766626290857981E-4	0.1406630343920082
>5k	0.0	0.0
>10k+	1.3833131454289905E-4	1.2057624210407645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30422	1.1796854611580554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013688415218880862	0.0
2	0.0	0.0	0.0	0.051651470457646766	0.0
3	0.0	0.0	0.0	0.10047219215898105	0.0
4	0.0	0.0	0.0	0.14669482938534362	0.0
5	0.0	0.0	0.0	0.2345255955914772	0.0
6	0.0	0.0	0.0	0.47603111962317696	0.0
7	0.0	0.0	0.0	0.6254015882439392	0.0
8	0.0	0.0	0.0	0.9662159830279162	0.0
9	0.0	0.0	0.0	1.1743341826872182	0.0
10	0.0	0.0	0.0	1.4182051269125489	0.0
11	0.0	0.0	0.0	1.5452786019048226	0.0
12	0.0	0.0	0.0	1.6830934112189941	0.0
13	0.0	0.0	0.0	1.7657280084751843	0.0
14	0.0	0.0	0.0	1.8105934373937258	0.0
15	0.0	0.0	0.0	1.8460359629179668	0.0
16	0.0	0.0	0.0	1.8987732000218704	0.0
17	0.0	0.0	0.0	1.9582964786648793	0.0
18	0.0	0.0	0.0	2.059893214850341	0.0
19	0.0	0.0	0.0	2.1113507984068702	0.0
20	0.0	0.0	0.0	2.1704863032476442	0.0
21	0.0	0.0	0.0	2.228574818822385	0.0
22	0.0	0.0	0.0	2.297210781817907	0.0
23	0.0	0.0	0.0	2.3733695565767796	0.0
24	0.0	0.0	0.0	2.4352970327936427	0.0
25	0.0	0.0	0.0	2.489391478205367	0.0
26	0.0	0.0	0.0	2.5433695914764214	0.0
27	0.0	0.0	0.0	2.6072359367044577	0.0
28	0.0	0.0	0.0	2.667728649853053	0.0
29	0.0	0.0	0.0	2.738768810422429	0.0
30	0.0	0.0	0.0	2.838310345456047	0.0
31	0.0	0.0	0.0	2.9198591760659807	0.0
32	0.0	0.0	0.0	3.00427753281245	0.0
33	0.0	0.0	0.0	3.0888897764600363	0.0
34	0.0	0.0	0.0	3.1748204510352203	0.0
35	0.0	0.0	0.0	3.266063626701018	0.0
36	0.0	0.0	0.0	3.3585089011537432	0.0
37	0.0	0.0	0.0	3.455219687431049	0.0
38	0.0	0.0	0.0	3.555187773647125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29115	0.0	43.17637	1
GCGTAAG	175	0.0	38.971424	1
GTTTTTT	32100	0.0	38.53084	2
TATCGCG	75	0.0	38.13333	1
CGTTAGG	285	0.0	37.82456	2
TCGTTAG	35	7.293729E-6	37.714287	1
CGTAAGG	385	0.0	37.714283	2
AGGGCGA	2635	0.0	36.73624	6
AAGGGAT	5185	0.0	36.235294	5
TAACGGG	770	0.0	35.999996	3
GCGCGAC	300	0.0	35.933334	9
TCGATAG	135	0.0	35.851852	1
AGGGATT	6035	0.0	35.76139	6
GGGCGAT	5260	0.0	35.760456	7
TAGGGAT	5150	0.0	35.541744	5
TACGTAG	155	0.0	35.483868	1
ACGGGAT	1445	0.0	35.47405	5
ATAGGGA	4020	0.0	35.24378	4
CGACGGA	25	0.0023532852	35.2	3
GCGAACG	100	0.0	35.2	1
>>END_MODULE