FastQCFastQC Report
Thu 26 May 2016
SRR1547164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547164_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2173992
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170420.7839035286238404No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26540.12207956607016032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144600.042.858921
CTATACG800.038.5000041
TAAGGGA33000.038.3333324
AGGGCGA23450.037.901926
GGCGATA11350.037.7973568
CGAGGGA15750.037.434924
CGACGGT2250.037.15555628
GGGCGAT45350.037.111367
GTTTTTT167400.036.9032252
GCGATGT5550.036.8648649
TAGGGCG9350.036.7058835
AGGGATT54650.036.6733786
GTTACGC301.3014773E-436.66666412
ACGGGAT13400.036.6119425
GCGTTCG1450.036.41379544
GCGATAC1450.036.4137959
CTCACGA2300.036.34782424
CGGTCTA2300.036.34782431
CACGGGC1950.036.1025664
AAGGGAT47000.035.9957475
Produced by FastQC (version 0.11.5)