FastQCFastQC Report
Thu 26 May 2016
SRR1547163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547163_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3064207
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412221.3452746501786597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT357200.043.0576671
GCGTAAG2550.039.6862751
TACGGGA12650.038.2608684
GTTTTTT397600.038.1292722
ACGGGAT16350.037.272175
GCGATAA3850.037.1428579
AGGGCGA32050.036.8611536
TAACGGG7050.036.8226933
AGGGATC40650.036.531376
AAGGGAT58750.036.360855
TAGGGAC32850.036.1643835
GGGCGAT64450.035.9100077
TAGGGAT55700.035.784565
CGGGATC9500.035.6631556
CGACGGT2600.035.53846428
TTCGTAG2300.035.3913041
ATAGGGC12900.035.3023264
CCGTACA250.002353354635.243
GTAGGGA41300.035.1573834
TAGGGCG12850.035.097285
Produced by FastQC (version 0.11.5)