##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547163_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3064207
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.693707050470156	31.0	30.0	31.0	28.0	34.0
2	31.04027861042025	31.0	31.0	33.0	28.0	34.0
3	31.127659782775773	31.0	31.0	34.0	28.0	34.0
4	34.98907776139145	35.0	35.0	37.0	32.0	37.0
5	34.82717747201804	35.0	35.0	37.0	32.0	37.0
6	34.87071010542042	35.0	35.0	37.0	32.0	37.0
7	35.49445549860045	36.0	35.0	37.0	35.0	37.0
8	34.45676679153856	37.0	35.0	37.0	33.0	37.0
9	36.59720834786945	39.0	37.0	39.0	32.0	39.0
10	36.07900119019374	38.0	35.0	39.0	31.0	39.0
11	35.83533031547803	37.0	35.0	39.0	30.0	39.0
12	35.70982835036928	37.0	35.0	39.0	30.0	39.0
13	35.68213635697588	37.0	35.0	39.0	30.0	39.0
14	36.663786421739786	38.0	35.0	40.0	31.0	41.0
15	36.76609706850745	38.0	35.0	40.0	31.0	41.0
16	36.76741714903726	38.0	35.0	40.0	31.0	41.0
17	36.675222659565755	38.0	35.0	40.0	31.0	41.0
18	36.48778591002501	38.0	35.0	40.0	30.0	41.0
19	36.340734813281216	38.0	35.0	40.0	30.0	41.0
20	36.10663052463492	38.0	34.0	40.0	30.0	41.0
21	35.96746564445548	38.0	34.0	40.0	30.0	41.0
22	35.924487477510496	37.0	34.0	40.0	30.0	41.0
23	35.79185055056659	37.0	34.0	40.0	30.0	41.0
24	35.67027782391986	37.0	34.0	40.0	29.0	41.0
25	35.490584676557425	37.0	34.0	40.0	29.0	41.0
26	35.35085194962351	37.0	34.0	40.0	28.0	41.0
27	35.16521925574872	37.0	34.0	40.0	27.0	41.0
28	35.161816744103774	37.0	34.0	40.0	27.0	41.0
29	35.001264274900485	37.0	33.0	40.0	27.0	41.0
30	34.91732118619924	37.0	33.0	40.0	26.0	41.0
31	34.75413181942343	37.0	33.0	40.0	25.0	41.0
32	34.311377462423394	37.0	33.0	40.0	23.0	41.0
33	33.89469281938198	37.0	33.0	40.0	21.0	41.0
34	33.552461697267844	37.0	32.0	40.0	18.0	41.0
35	33.20775391479753	37.0	32.0	40.0	18.0	41.0
36	33.094904815503654	37.0	31.0	40.0	16.0	41.0
37	33.06003249780449	37.0	31.0	40.0	15.0	41.0
38	33.04325034176868	37.0	31.0	40.0	15.0	41.0
39	32.92294417446341	37.0	31.0	40.0	15.0	41.0
40	32.857443704031745	37.0	31.0	40.0	15.0	41.0
41	32.8552744641599	37.0	31.0	40.0	15.0	41.0
42	32.704758523167655	36.0	31.0	40.0	14.0	41.0
43	32.74214046244265	36.0	31.0	40.0	14.0	41.0
44	32.45202690288222	36.0	30.0	40.0	12.0	41.0
45	32.403726967531895	36.0	30.0	40.0	12.0	41.0
46	32.360688752424366	36.0	30.0	40.0	12.0	41.0
47	32.30777946790148	36.0	30.0	40.0	12.0	41.0
48	32.28608804822912	36.0	30.0	40.0	12.0	41.0
49	32.29342175642834	36.0	30.0	40.0	12.0	41.0
50	31.766974620187213	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	7.0
13	14.0
14	57.0
15	139.0
16	492.0
17	1260.0
18	3110.0
19	6016.0
20	9984.0
21	15178.0
22	22127.0
23	31653.0
24	44590.0
25	62758.0
26	86131.0
27	98530.0
28	99049.0
29	100274.0
30	109522.0
31	127360.0
32	150682.0
33	182041.0
34	236870.0
35	273536.0
36	274264.0
37	322879.0
38	395570.0
39	410110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.843284086225246	22.60438018710877	25.72489391219327	21.827441814472717
2	36.89662610913688	23.97915676062355	26.41854809417249	12.70566903606708
3	26.133580401062982	23.930465533170572	37.184041417567414	12.75191264819903
4	22.771894979679896	26.643304450384715	36.86882119909001	13.715979370845377
5	20.49182708609438	30.56954050428055	35.87548099720417	13.063151412420899
6	19.141102412467564	38.48196287000193	33.192046098713305	9.184888618817201
7	82.35997111161224	2.7061487686700016	13.852686845242504	1.0811932744752557
8	80.71132270110995	4.948001228376542	13.171564453706946	1.169111616806567
9	77.59619372973171	5.1786971311011305	15.039062308780052	2.1860468303871117
10	33.18424636455696	35.509611459016966	20.966631823502784	10.339510352923286
11	23.910068738828677	24.210962248960335	36.328159292110485	15.550809720100503
12	23.768433398918546	22.7047324152709	36.7291439514367	16.797690234373853
13	20.800618234995223	25.56730012039004	37.30684643694111	16.325235207673632
14	18.44245509523345	28.19982462020353	36.402012005063625	16.955708279499394
15	17.973589904337402	24.87504923786154	40.85086288230528	16.30049797549578
16	20.576840924911405	23.742390771902812	39.458300304124364	16.222467999061422
17	20.415396218336422	23.10894792682087	35.99104107522762	20.484614779615086
18	21.262140579928186	23.298850240861665	37.49762989249747	17.94137928671268
19	23.125200092552493	24.37191743247111	34.832307347382205	17.670575127594187
20	24.343655634230977	24.45432048161237	34.21766218796576	16.98436169619089
21	22.644357904018886	24.837714945498135	35.796341435157615	16.721585715325368
22	22.00272370632924	23.142561843896317	35.56678775291617	19.287926696858275
23	19.343471247210125	26.40888817237217	35.724512084203184	18.523128496214518
24	19.12902751021716	24.63955600910774	38.19604876563496	18.03536771504014
25	19.23071776808812	25.92817652332235	36.564794741347434	18.276310967242097
26	19.25333373365442	27.382092658883682	34.73704615908781	18.62752744837408
27	18.699878957263657	26.361991862821277	36.22637112962669	18.711758050288378
28	18.205297488061348	26.17499405229477	38.00506950085291	17.61463895879097
29	19.20829761174751	24.820092115186736	37.592336287985766	18.379273985079987
30	19.776307540580646	25.213146500872817	36.67931050350058	18.331235455045956
31	21.530888742177012	25.594028079695658	34.76276243739408	18.11232074073325
32	21.985492494469206	25.70407286452906	34.51842515861363	17.792009482388103
33	21.64899434013433	26.315813520431224	33.35574261138363	18.679449528050814
34	19.807147493625592	27.230634222818495	33.65862684864306	19.30359143491285
35	19.850682411468938	27.681843948532197	33.16851635676049	19.298957283238373
36	22.00252789710356	28.156877130037234	30.86883490573581	18.9717600671234
37	20.806623051249478	29.486780755999842	30.950650527200025	18.755945665550662
38	20.87779970478496	29.575743414201455	30.210850637701697	19.335606243311894
39	20.73567484181062	28.821518911744537	30.3912562042969	20.051550042147937
40	21.63678889840014	27.600485215261237	30.637257861495648	20.12546802484297
41	19.80972564843041	27.690720633429788	30.690974859074466	21.80857885906533
42	20.804958672831177	28.35160940497819	29.696329262350744	21.147102659839888
43	20.8745688525612	27.74026036752739	30.470199957117778	20.91497082279363
44	20.434095999389076	28.431989092120734	31.207030073359928	19.926884835130263
45	19.88684184847825	29.27064000571763	30.460996923510713	20.381521222293404
46	20.60947579585844	29.235459614836728	30.209120989541503	19.945943599763332
47	20.466567695981375	28.32964613683083	30.766230871478328	20.437555295709462
48	20.72467689030147	27.40350113422494	31.722758938935915	20.149063036537676
49	20.949726960352223	26.89596362125666	31.89973784408168	20.254571574309438
50	19.709047071558807	28.672377551516593	31.336459971535866	20.282115405388733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1680.0
1	2754.5
2	3829.0
3	40712.0
4	77595.0
5	58499.0
6	39403.0
7	39310.0
8	39217.0
9	39373.5
10	39530.0
11	38902.0
12	38274.0
13	37035.5
14	35797.0
15	33867.5
16	31938.0
17	30148.5
18	28359.0
19	26763.0
20	25167.0
21	24583.0
22	23999.0
23	25843.0
24	27687.0
25	30884.5
26	34082.0
27	38575.0
28	43068.0
29	51939.5
30	60811.0
31	69628.0
32	78445.0
33	87980.0
34	97515.0
35	108410.0
36	119305.0
37	131361.5
38	143418.0
39	154346.0
40	165274.0
41	178640.0
42	192006.0
43	206622.0
44	221238.0
45	222035.0
46	222832.0
47	222063.0
48	221294.0
49	208940.0
50	196586.0
51	186749.0
52	176912.0
53	165167.5
54	153423.0
55	140954.5
56	128486.0
57	118063.5
58	107641.0
59	97800.5
60	87960.0
61	78355.0
62	68750.0
63	58406.0
64	48062.0
65	39578.0
66	31094.0
67	25193.5
68	19293.0
69	16577.5
70	13862.0
71	11045.5
72	8229.0
73	6841.5
74	5454.0
75	4276.5
76	3099.0
77	2338.0
78	1577.0
79	1302.5
80	1028.0
81	823.0
82	618.0
83	429.5
84	241.0
85	161.0
86	81.0
87	55.0
88	29.0
89	21.0
90	13.0
91	7.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3064207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.665183409570982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67261108104721	21.48827031457446
2	5.606051493956456	3.1018488556760953
3	2.424301767872546	2.0120625914502344
4	1.580373660526406	1.7488530869647239
5	1.1468338855028029	1.5863684891372984
6	0.8829832652575493	1.465673638855918
7	0.7678051148796076	1.4869028528165744
8	0.6541634542535506	1.4478041553410381
9	0.5970953489085735	1.4866877106501717
>10	8.038205783358512	50.480564500125894
>50	0.5837787066320539	10.059656260751728
>100	0.044112100542087175	1.9323686480060072
>500	0.0012030980447463775	0.2013011969528539
>1k	3.6092941342366955E-4	0.12960761589516545
>5k	0.0	0.0
>10k+	1.203098044745565E-4	1.3720300828018772
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41222	1.3452746501786597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018340797472233435	0.0
2	0.0	0.0	0.0	0.061712540960842395	0.0
3	0.0	0.0	0.0	0.11882356511815292	0.0
4	0.0	0.0	0.0	0.1638923218960077	0.0
5	0.0	0.0	0.0	0.24378248597434835	0.0
6	0.0	0.0	0.0	0.45848730193488885	0.0
7	0.0	0.0	0.0	0.5885698975297687	0.0
8	0.0	0.0	0.0	0.888973884597222	0.0
9	0.0	0.0	0.0	1.0621997795840816	0.0
10	0.0	0.0	0.0	1.278536339091974	0.0
11	0.0	0.0	0.0	1.4072156352361311	0.0
12	0.0	0.0	0.0	1.5380488328627928	0.0
13	0.0	0.0	0.0	1.6252165731623223	0.0
14	0.0	0.0	0.0	1.676877573871478	0.0
15	0.0	0.0	0.0	1.7167900210397014	0.0
16	0.0	0.0	0.0	1.7733136175199653	0.0
17	0.0	0.0	0.0	1.8356788558997483	0.0
18	0.0	0.0	0.0	1.9435697392506446	0.0
19	0.0	0.0	0.0	1.9941864240894953	0.0
20	0.0	0.0	0.0	2.0500899580217653	0.0
21	0.0	0.0	0.0	2.107951584210858	0.0
22	0.0	0.0	0.0	2.1728949773954565	0.0
23	0.0	0.0	0.0	2.245964453445867	0.0
24	0.0	0.0	0.0	2.3092108333412202	0.0
25	0.0	0.0	0.0	2.3698464235608103	0.0
26	0.0	0.0	0.0	2.4306125532641887	3.2634870947034585E-5
27	0.0	0.0	0.0	2.499504765833379	3.2634870947034585E-5
28	0.0	0.0	0.0	2.572476337270948	3.2634870947034585E-5
29	0.0	0.0	0.0	2.649005109641744	3.2634870947034585E-5
30	0.0	0.0	0.0	2.7443968374199263	3.2634870947034585E-5
31	0.0	0.0	0.0	2.8268977911740296	3.2634870947034585E-5
32	0.0	0.0	0.0	2.9153709263114402	3.2634870947034585E-5
33	0.0	0.0	0.0	3.00253866661097	3.2634870947034585E-5
34	0.0	0.0	0.0	3.0907507227808044	3.2634870947034585E-5
35	0.0	0.0	0.0	3.189536477137478	3.2634870947034585E-5
36	0.0	0.0	0.0	3.286951566914376	3.2634870947034585E-5
37	0.0	0.0	0.0	3.385084623852109	3.2634870947034585E-5
38	0.0	0.0	0.0	3.4912784939137596	3.2634870947034585E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35720	0.0	43.057667	1
GCGTAAG	255	0.0	39.686275	1
TACGGGA	1265	0.0	38.260868	4
GTTTTTT	39760	0.0	38.129272	2
ACGGGAT	1635	0.0	37.27217	5
GCGATAA	385	0.0	37.142857	9
AGGGCGA	3205	0.0	36.861153	6
TAACGGG	705	0.0	36.822693	3
AGGGATC	4065	0.0	36.53137	6
AAGGGAT	5875	0.0	36.36085	5
TAGGGAC	3285	0.0	36.164383	5
GGGCGAT	6445	0.0	35.910007	7
TAGGGAT	5570	0.0	35.78456	5
CGGGATC	950	0.0	35.663155	6
CGACGGT	260	0.0	35.538464	28
TTCGTAG	230	0.0	35.391304	1
ATAGGGC	1290	0.0	35.302326	4
CCGTACA	25	0.0023533546	35.2	43
GTAGGGA	4130	0.0	35.157383	4
TAGGGCG	1285	0.0	35.09728	5
>>END_MODULE