FastQCFastQC Report
Thu 26 May 2016
SRR1547162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547162_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4792287
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163340.34083935290186085No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103240.21542950161373892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192800.041.14731
ACGAGAT16350.040.501535
GAGCGAT28600.040.461547
TCGCCTA357.295039E-637.71428723
CGCGAAC357.295039E-637.71428726
TCGACCC357.295039E-637.71428744
GGGCGAT51850.037.2960477
AGCGATC4100.037.024398
ACGGGAT25900.036.8648645
GCGCGAC4850.036.7422689
GCGATAT4200.036.6666689
CTCGACC301.301711E-436.66666441
TACGGGA15100.036.5695344
CGAGATT19950.036.1704256
ACGTTGA1950.036.1025662
GCGAACG1950.036.1025661
GGCGATA13050.035.9080478
GACCGAT25800.035.8992239
AAGGGAT87500.035.7782865
AGAGATT81900.035.7264986
Produced by FastQC (version 0.11.5)