##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547162_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4792287
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60626857281294	31.0	31.0	33.0	30.0	34.0
2	31.99495355766464	31.0	31.0	34.0	30.0	34.0
3	32.12275120417454	33.0	31.0	34.0	30.0	34.0
4	35.79629099843144	37.0	35.0	37.0	35.0	37.0
5	35.70113246556394	37.0	35.0	37.0	33.0	37.0
6	35.76155601699147	37.0	35.0	37.0	35.0	37.0
7	36.107102308355074	37.0	35.0	37.0	35.0	37.0
8	22.062669660644282	35.0	0.0	37.0	0.0	37.0
9	30.001298336264085	37.0	18.0	39.0	17.0	39.0
10	34.46067962957978	37.0	32.0	39.0	27.0	39.0
11	36.082638205933826	37.0	35.0	39.0	32.0	39.0
12	36.709555792464016	38.0	35.0	39.0	33.0	39.0
13	36.913290042937746	39.0	37.0	39.0	33.0	39.0
14	38.09038252508667	40.0	37.0	41.0	33.0	41.0
15	38.27153298623392	40.0	37.0	41.0	33.0	41.0
16	38.28737677856105	40.0	37.0	41.0	33.0	41.0
17	38.22249189165841	40.0	37.0	41.0	33.0	41.0
18	38.15851304398088	40.0	37.0	41.0	33.0	41.0
19	38.18998236124005	40.0	37.0	41.0	34.0	41.0
20	38.10942103425776	40.0	37.0	41.0	34.0	41.0
21	38.03130342569216	40.0	36.0	41.0	33.0	41.0
22	37.996090384403104	40.0	36.0	41.0	33.0	41.0
23	37.866489214857125	40.0	36.0	41.0	33.0	41.0
24	37.78050375530514	40.0	36.0	41.0	33.0	41.0
25	37.65277956015573	40.0	36.0	41.0	33.0	41.0
26	37.60886670602157	40.0	36.0	41.0	33.0	41.0
27	37.506454433968585	40.0	35.0	41.0	33.0	41.0
28	37.51212124816398	40.0	36.0	41.0	33.0	41.0
29	37.42901207711475	39.0	36.0	41.0	33.0	41.0
30	37.32574468098426	39.0	36.0	41.0	32.0	41.0
31	37.26492298979589	39.0	35.0	41.0	32.0	41.0
32	37.0894850412757	39.0	35.0	41.0	31.0	41.0
33	36.98244679419242	40.0	35.0	41.0	31.0	41.0
34	36.90591569327964	40.0	35.0	41.0	31.0	41.0
35	36.74354624420449	40.0	35.0	41.0	30.0	41.0
36	36.65835643816825	40.0	35.0	41.0	30.0	41.0
37	36.63701130587546	40.0	35.0	41.0	30.0	41.0
38	36.54427374654314	39.0	35.0	41.0	30.0	41.0
39	36.44587292038227	39.0	35.0	41.0	30.0	41.0
40	36.404910640785914	39.0	35.0	41.0	30.0	41.0
41	36.3872837749492	39.0	35.0	41.0	30.0	41.0
42	36.167608283894516	39.0	35.0	41.0	29.0	41.0
43	36.22284662834259	39.0	35.0	41.0	30.0	41.0
44	36.04342769955138	39.0	35.0	41.0	29.0	41.0
45	35.851667272849056	39.0	35.0	40.0	28.0	41.0
46	35.915967887566	39.0	35.0	40.0	29.0	41.0
47	35.795203834828754	39.0	35.0	40.0	28.0	41.0
48	35.804690537106815	38.0	35.0	40.0	28.0	41.0
49	35.71307957974971	38.0	35.0	40.0	28.0	41.0
50	35.281004038364145	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	7.0
13	17.0
14	24.0
15	63.0
16	170.0
17	400.0
18	1029.0
19	2306.0
20	4419.0
21	7481.0
22	11992.0
23	18517.0
24	27506.0
25	40079.0
26	53977.0
27	63283.0
28	69254.0
29	78984.0
30	96087.0
31	123258.0
32	159484.0
33	211923.0
34	311956.0
35	453710.0
36	465247.0
37	720164.0
38	887788.0
39	983157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00875198000454	23.33126125376047	28.227316101894566	14.432670664340428
2	31.86731095195259	25.444636350034962	29.6057393891476	13.08231330886485
3	29.167389181824877	25.745119188395854	31.693343908659894	13.394147721119372
4	25.115106837299184	28.55517626552834	31.355968455144694	14.973748442027784
5	22.941843841990263	33.13513986119779	29.861462804710985	14.061553492100954
6	21.128868116621565	41.45432024417569	26.672087877875427	10.744723761327316
7	89.38757215500658	2.8315082130932474	6.337892534399546	1.4430270975006296
8	55.11890251981987	39.96862041025506	3.635967545349433	1.276509524575636
9	84.61880517590036	5.635075695591688	7.480728929632136	2.2653901988758185
10	32.530251214086306	45.279404175918515	12.735318231149344	9.455026378845842
11	25.08399434341057	22.845418064485703	35.60581826589267	16.464769326211055
12	25.272046519751424	23.539575154826913	33.68122568619116	17.507152639230497
13	21.411092449179275	28.172937054896753	33.864040279724485	16.55193021619949
14	17.93252365728513	31.888010880817447	32.23661270704363	17.94285275485379
15	17.283668528199584	28.414387535637996	37.88329037889425	16.41865355726817
16	21.096253208541142	26.88868592386057	35.78306140679805	16.23199946080024
17	21.03717911719394	25.509177559691228	31.147383284849173	22.306260038265656
18	21.975207244474298	26.339678737938694	33.23513387240789	18.44998014517912
19	24.02126583821044	28.10958525647567	29.660598374012242	18.20855053130165
20	25.392615258643737	27.718394161284582	29.295156988719583	17.593833591352105
21	23.554912299701584	27.144492806879057	31.260481686510012	18.040113206909353
22	22.75813197331462	24.604265145221895	31.90299328900794	20.734609592455545
23	19.877106692483153	28.936226064924742	30.826221384487194	20.36044585810491
24	20.02221486317493	27.67171498701977	33.94408556916562	18.36198458063968
25	20.307214488614726	28.96969651441994	31.387206150215963	19.33588284674937
26	19.55849054950173	31.188198870393197	29.361638816706932	19.891671763398143
27	19.320524835010925	29.22429311933947	31.670912030101704	19.7842700155479
28	18.14505266483414	29.032860511067053	34.31626695145763	18.505819872641183
29	19.473708481983653	26.581922159503385	33.64072310360377	20.30364625490919
30	20.708526012736716	27.318522450763073	32.70916370409368	19.263787832406532
31	23.23297832537993	27.175897436860524	29.644572622632992	19.946551615126555
32	23.04442534430847	27.872996754994013	30.126889311929773	18.955688588767742
33	22.07472131781757	28.138715398305653	29.581053889301707	20.205509394575074
34	20.228922015730692	28.570179540582608	31.378066463882487	19.822831979804214
35	19.846870606873086	28.264813856098353	31.46349540417759	20.424820132850975
36	23.079711210952098	28.19340744826009	29.99019048733934	18.73669085344847
37	21.146145879827312	29.222164699234416	30.177992261314902	19.45369715962337
38	21.035906238503664	29.763847615971244	28.974641961134633	20.22560418439046
39	21.070086161367215	29.076305321446732	29.430499467164633	20.42310905002142
40	22.029523690880783	27.89042058624619	29.528365058269674	20.55169066460335
41	20.10511891295325	27.261034241062777	29.938816268725144	22.69503057725883
42	21.917447765544924	28.571014215133612	28.295926349986967	21.215611669334493
43	21.681840006660703	27.680458202941516	29.134398670196504	21.503303120201274
44	20.881449712840656	29.395589204068955	29.080520427929297	20.642440655161096
45	20.151902421536942	29.341585760619093	28.441890062093528	22.064621755750437
46	21.77494795282503	29.159939711457184	28.712929755667805	20.352182580049984
47	21.136505388763236	28.56467068854599	29.60081480929669	20.698009113394086
48	21.38753375997723	26.77859235058334	30.57880298070629	21.25507090873314
49	21.046047534298342	26.600055464123912	30.668447027483953	21.68544997409379
50	20.219865796852318	28.59451030374433	30.268387515188465	20.917236384214885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3144.0
1	4211.5
2	5279.0
3	26174.5
4	47070.0
5	33889.0
6	20708.0
7	21012.5
8	21317.0
9	22493.0
10	23669.0
11	24136.0
12	24603.0
13	24732.0
14	24861.0
15	24535.5
16	24210.0
17	24294.0
18	24378.0
19	25949.0
20	27520.0
21	30960.5
22	34401.0
23	41964.5
24	49528.0
25	61097.5
26	72667.0
27	87011.5
28	101356.0
29	115402.5
30	129449.0
31	144475.0
32	159501.0
33	175477.0
34	191453.0
35	211505.5
36	231558.0
37	246893.5
38	262229.0
39	283646.5
40	305064.0
41	331419.0
42	357774.0
43	375672.5
44	393571.0
45	400700.5
46	407830.0
47	387958.5
48	368087.0
49	342772.5
50	317458.0
51	293295.5
52	269133.0
53	241119.5
54	213106.0
55	195563.5
56	178021.0
57	162447.0
58	146873.0
59	131265.0
60	115657.0
61	103036.5
62	90416.0
63	74797.5
64	59179.0
65	47242.5
66	35306.0
67	28362.5
68	21419.0
69	17513.5
70	13608.0
71	11252.0
72	8896.0
73	7327.5
74	5759.0
75	4712.5
76	3666.0
77	2623.5
78	1581.0
79	1093.0
80	605.0
81	418.5
82	232.0
83	149.5
84	67.0
85	53.5
86	40.0
87	32.5
88	25.0
89	16.0
90	7.0
91	6.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4792287.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.604673870934086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09478589429395	18.67006625926166
2	7.1822788106740125	3.3907069795216085
3	2.4738062950594815	1.7518017244422839
4	1.3027440325626436	1.2300339210378695
5	0.8654112786889714	1.0213875498840608
6	0.6239922509831636	0.8837480149468575
7	0.48351055965771333	0.7989176351711199
8	0.3996864527677963	0.7547574694571466
9	0.3505045616713243	0.7446191281673682
>10	5.06498879752347	29.935736788210775
>50	1.8533979097214552	30.39370968900593
>100	0.30149251133534777	9.063066516797301
>500	0.0023379434792587424	0.37607018599994785
>1k	8.501612651764642E-4	0.3166695589665763
>5k	0.0	0.0
>10k+	2.1254031629411606E-4	0.6687085791295144
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16334	0.34083935290186085	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10324	0.21542950161373892	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02877540514581034	0.0
2	0.0	0.0	0.0	0.09799079228769061	0.0
3	0.0	0.0	0.0	0.16276153744548272	0.0
4	0.0	0.0	0.0	0.24950509015841496	0.0
5	0.0	0.0	0.0	0.41525058912373153	0.0
6	2.0866863775061886E-5	0.0	0.0	0.7285039481149606	0.0
7	2.0866863775061886E-5	0.0	0.0	0.8737581868531663	0.0
8	4.173372755012377E-5	0.0	0.0	1.22903740948737	0.0
9	4.173372755012377E-5	0.0	0.0	1.5178974047255518	0.0
10	4.173372755012377E-5	0.0	0.0	1.9450003724735183	0.0
11	4.173372755012377E-5	0.0	0.0	2.3168478849451213	0.0
12	4.173372755012377E-5	0.0	0.0	2.637821983533123	0.0
13	4.173372755012377E-5	0.0	0.0	2.8150233907109485	0.0
14	4.173372755012377E-5	0.0	0.0	2.9113657007604092	0.0
15	4.173372755012377E-5	0.0	0.0	2.9979631854269164	0.0
16	6.260059132518565E-5	0.0	0.0	3.151209432990971	0.0
17	6.260059132518565E-5	0.0	0.0	3.351614792686665	0.0
18	6.260059132518565E-5	0.0	0.0	3.5959657674926397	0.0
19	6.260059132518565E-5	0.0	0.0	3.7398845269492416	0.0
20	6.260059132518565E-5	0.0	0.0	3.890188546721012	0.0
21	6.260059132518565E-5	0.0	0.0	4.063717385874427	0.0
22	6.260059132518565E-5	0.0	0.0	4.264435748526747	0.0
23	8.346745510024755E-5	0.0	0.0	4.4699743567111065	0.0
24	8.346745510024755E-5	0.0	0.0	4.632506358655064	0.0
25	8.346745510024755E-5	0.0	0.0	4.771145801576575	0.0
26	8.346745510024755E-5	0.0	0.0	4.910035646863387	0.0
27	8.346745510024755E-5	0.0	0.0	5.055582021694444	0.0
28	8.346745510024755E-5	0.0	0.0	5.214065852066039	0.0
29	8.346745510024755E-5	0.0	0.0	5.3809590285389834	0.0
30	8.346745510024755E-5	0.0	0.0	5.5802584444546	0.0
31	8.346745510024755E-5	0.0	0.0	5.757084248084474	0.0
32	8.346745510024755E-5	0.0	0.0	5.941672525038672	0.0
33	8.346745510024755E-5	0.0	0.0	6.1197086067674995	0.0
34	8.346745510024755E-5	0.0	0.0	6.296158806849423	0.0
35	8.346745510024755E-5	0.0	0.0	6.495312154718613	0.0
36	8.346745510024755E-5	0.0	0.0	6.684365940520674	0.0
37	8.346745510024755E-5	0.0	0.0	6.87513080915229	0.0
38	8.346745510024755E-5	0.0	0.0	7.083152574125882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	19280	0.0	41.1473	1
ACGAGAT	1635	0.0	40.50153	5
GAGCGAT	2860	0.0	40.46154	7
TCGCCTA	35	7.295039E-6	37.714287	23
CGCGAAC	35	7.295039E-6	37.714287	26
TCGACCC	35	7.295039E-6	37.714287	44
GGGCGAT	5185	0.0	37.296047	7
AGCGATC	410	0.0	37.02439	8
ACGGGAT	2590	0.0	36.864864	5
GCGCGAC	485	0.0	36.742268	9
GCGATAT	420	0.0	36.666668	9
CTCGACC	30	1.301711E-4	36.666664	41
TACGGGA	1510	0.0	36.569534	4
CGAGATT	1995	0.0	36.170425	6
ACGTTGA	195	0.0	36.102566	2
GCGAACG	195	0.0	36.102566	1
GGCGATA	1305	0.0	35.908047	8
GACCGAT	2580	0.0	35.899223	9
AAGGGAT	8750	0.0	35.778286	5
AGAGATT	8190	0.0	35.726498	6
>>END_MODULE