FastQCFastQC Report
Thu 26 May 2016
SRR1547161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547161_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1131434
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.1939132110224724No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.11463328837563658No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.85643E-444.01
TCGATAG800.044.01
ACTAACG254.4427965E-544.024
GCGTAAT207.85643E-444.036
TACGCGG700.040.8571432
CGGTCTA1400.040.85714331
CGTTTTT27450.040.5537341
ATTACGA557.8216544E-1140.02
TCGTAAG452.3508619E-839.111111
TGATTCG850.038.8235315
ACAACGA1450.037.93103413
GAGCGAT6850.037.8978087
GTACCGG357.2901057E-637.7142872
GCGTAAG651.0913936E-1137.2307661
ACGGGCC601.9826984E-1036.6666685
TCTACGA301.3010822E-436.6666682
CGTAAGG900.036.6666682
CGATACG301.3010822E-436.66666810
GTAGGGC3800.035.8947374
ATAGGGC4500.035.688894
Produced by FastQC (version 0.11.5)