##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547161_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1131434 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48879828606883 31.0 31.0 33.0 30.0 34.0 2 31.87879717243781 31.0 31.0 34.0 30.0 34.0 3 32.036985807391325 33.0 31.0 34.0 30.0 34.0 4 35.72591154234361 37.0 35.0 37.0 35.0 37.0 5 35.626213283320105 37.0 35.0 37.0 33.0 37.0 6 35.67994421238888 37.0 35.0 37.0 33.0 37.0 7 36.00143004364373 37.0 35.0 37.0 35.0 37.0 8 22.10926841512629 35.0 0.0 37.0 0.0 37.0 9 29.979769036461693 37.0 18.0 39.0 17.0 39.0 10 34.315947726513436 35.0 32.0 39.0 27.0 39.0 11 35.92667004880532 37.0 35.0 39.0 32.0 39.0 12 36.416786131581695 37.0 35.0 39.0 32.0 39.0 13 36.57217919914021 39.0 35.0 39.0 33.0 39.0 14 37.68114357532123 39.0 36.0 41.0 33.0 41.0 15 37.90368682574503 40.0 36.0 41.0 33.0 41.0 16 37.95178242831663 40.0 36.0 41.0 33.0 41.0 17 37.902046429575215 40.0 36.0 41.0 33.0 41.0 18 37.869957947171464 40.0 36.0 41.0 33.0 41.0 19 37.905253863680954 40.0 36.0 41.0 33.0 41.0 20 37.826279747647675 40.0 36.0 41.0 33.0 41.0 21 37.730664802365844 40.0 36.0 41.0 33.0 41.0 22 37.69497823116505 40.0 36.0 41.0 33.0 41.0 23 37.563123434508775 39.0 35.0 41.0 33.0 41.0 24 37.489530100739415 39.0 35.0 41.0 32.0 41.0 25 37.341040661673595 39.0 35.0 41.0 32.0 41.0 26 37.293790004542906 39.0 35.0 41.0 32.0 41.0 27 37.18723761173873 39.0 35.0 41.0 32.0 41.0 28 37.19218177993591 39.0 35.0 41.0 32.0 41.0 29 37.089473181820594 39.0 35.0 41.0 32.0 41.0 30 36.990657873106166 39.0 35.0 41.0 31.0 41.0 31 36.945544326933785 39.0 35.0 41.0 31.0 41.0 32 36.785957466365694 39.0 35.0 41.0 31.0 41.0 33 36.7027480171181 39.0 35.0 41.0 31.0 41.0 34 36.63086490241587 39.0 35.0 41.0 30.0 41.0 35 36.48379401714992 39.0 35.0 41.0 30.0 41.0 36 36.3995964413302 39.0 35.0 41.0 30.0 41.0 37 36.35453592520642 39.0 35.0 41.0 30.0 41.0 38 36.26956234300896 39.0 35.0 41.0 30.0 41.0 39 36.17301672037432 39.0 35.0 41.0 30.0 41.0 40 36.10162766895815 39.0 35.0 40.0 29.0 41.0 41 36.10395126892068 39.0 35.0 40.0 30.0 41.0 42 35.886020748890346 38.0 35.0 40.0 29.0 41.0 43 35.93427720927602 38.0 35.0 40.0 29.0 41.0 44 35.73438220877223 38.0 35.0 40.0 28.0 41.0 45 35.51648615827348 38.0 34.0 40.0 27.0 41.0 46 35.55805376186326 38.0 34.0 40.0 28.0 41.0 47 35.404598058746686 38.0 34.0 40.0 27.0 41.0 48 35.41363968203183 38.0 34.0 40.0 28.0 41.0 49 35.312690797695666 38.0 34.0 40.0 27.0 41.0 50 34.87227977946571 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 10.0 13 1.0 14 9.0 15 29.0 16 45.0 17 145.0 18 284.0 19 688.0 20 1255.0 21 1995.0 22 3101.0 23 4699.0 24 6992.0 25 9651.0 26 12615.0 27 15206.0 28 17315.0 29 20505.0 30 25473.0 31 32602.0 32 42243.0 33 55377.0 34 84286.0 35 122351.0 36 114028.0 37 170647.0 38 196449.0 39 193431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.78164170424435 22.70207541933511 27.67452630909094 12.841756567329602 2 32.178633486354485 25.36303487432762 28.675468476287612 13.782863163030278 3 31.031151618211933 25.569321763355173 29.60561552861236 13.79391108982053 4 26.362562906895143 28.882108898972454 29.073105457322303 15.6822227368101 5 23.80041610911463 32.81791072214553 28.233993321749214 15.147679846990632 6 20.941742956283797 42.03161651497126 26.3049369207572 10.72170360798774 7 88.26162197706627 3.9539204231090808 5.8236715530910335 1.9607860467336142 8 54.63491463046011 40.1284564543756 3.512180118327715 1.7244487968365807 9 83.47804644371656 5.131541035535435 7.930378616870272 3.4600339038777337 10 42.7182672608389 27.263189898836345 15.482034303370767 14.53650853695399 11 33.84466084632422 26.737308583620433 23.056050993694726 16.361979576360618 12 31.395468052047228 23.813054937362672 26.847257551037 17.9442194595531 13 23.803509528615898 30.38542239317539 27.683541417351787 18.12752666085693 14 20.304675305850807 32.48470525015158 28.778788687630037 18.431830756367585 15 19.33616985171031 26.420100509618766 36.5308095743985 17.71292006427242 16 21.57200508381399 24.27556534450971 34.8528504534953 19.299579118180997 17 21.460818748596914 24.652520606593047 27.791545949653273 26.095114695156767 18 23.347893027785975 25.345623341706187 30.682920965783246 20.623562664724588 19 26.73333132997594 27.36580304286419 25.843045197510417 20.057820429649453 20 28.230988285662267 26.512019260513647 25.678121746385564 19.578870707438526 21 25.398476623470746 27.776697536047173 27.286257969974386 19.53856787050769 22 24.382155742182047 24.965397893292938 27.098708364783096 23.55373799974192 23 22.212254537162572 29.106691154764665 26.041200812420346 22.63985349565242 24 21.958770904887075 27.375348451610964 30.87294530657555 19.792935336926416 25 21.971586499963763 27.286257969974386 29.104304802577968 21.637850727483883 26 20.90082143545271 30.33362971238269 26.399683940910386 22.365864911254214 27 21.217234058725477 28.903232534995414 28.082857683258588 21.79667572302052 28 19.77649602186252 29.00876233169588 31.21914314047483 19.995598505966765 29 20.77187003395691 26.601286508978873 30.70174663303383 21.92509682403039 30 22.156926519797 27.685220702223905 29.18791551252658 20.969937265452515 31 26.05551892554051 27.504741770178377 25.650457737702776 20.789281566578342 32 26.174041084146317 27.513580111610576 25.805835780080855 20.50654302416226 33 25.55076124634755 27.41264625245485 25.559864738022718 21.476727763174875 34 21.863493584247955 27.907151455586447 28.277654728424285 21.951700231741313 35 21.45887431348183 27.796583804269627 28.66786750265592 22.076674379592625 36 26.311477293417024 27.716596814308215 26.075405193762958 19.8965206985118 37 22.693413844731552 29.825159929788214 27.257179826662448 20.224246398817783 38 22.674146260409355 30.325763588508035 25.768007678751037 21.232082472331573 39 21.890627292444808 29.62620886414939 26.087160187867788 22.396003655538017 40 23.718661450866776 26.6166652230709 27.15801363579316 22.506659690269164 41 20.005320681542184 26.63389998886369 27.871532939614685 25.489246389979442 42 22.615901590371156 28.004019677683367 25.81697209028542 23.563106641660053 43 22.77587557029398 27.552733964155223 26.374494667828614 23.296895797722183 44 21.894339395846334 29.23546578943182 27.02375922943804 21.846435585283807 45 21.673646010284294 29.487800437321134 26.005493913034254 22.833059639360318 46 23.235822858425678 28.60776678091696 27.034276855742355 21.122133504915002 47 22.25715331163815 28.330508010188836 27.38118175695622 22.031156921216795 48 23.24784300277347 26.621261160615646 28.044145747785553 22.086750088825333 49 22.406609665256656 25.974471334607234 28.757134751121143 22.861784249014967 50 21.085807921628657 28.787538645647913 28.18343800875703 21.9432154239664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 691.0 1 1044.5 2 1398.0 3 4085.5 4 6773.0 5 5129.0 6 3485.0 7 3566.0 8 3647.0 9 3889.5 10 4132.0 11 4339.5 12 4547.0 13 4440.0 14 4333.0 15 4403.5 16 4474.0 17 4559.5 18 4645.0 19 4642.0 20 4639.0 21 5268.0 22 5897.0 23 6492.0 24 7087.0 25 8170.5 26 9254.0 27 10996.0 28 12738.0 29 15005.0 30 17272.0 31 20421.0 32 23570.0 33 27407.5 34 31245.0 35 34702.5 36 38160.0 37 41479.5 38 44799.0 39 51970.0 40 59141.0 41 67502.5 42 75864.0 43 81982.0 44 88100.0 45 91007.0 46 93914.0 47 96300.5 48 98687.0 49 96937.5 50 95188.0 51 87086.5 52 78985.0 53 71806.5 54 64628.0 55 59941.0 56 55254.0 57 51622.0 58 47990.0 59 43622.0 60 39254.0 61 35263.5 62 31273.0 63 27296.5 64 23320.0 65 19541.5 66 15763.0 67 13403.5 68 11044.0 69 9560.5 70 8077.0 71 6528.0 72 4979.0 73 4022.0 74 3065.0 75 2531.5 76 1998.0 77 1578.0 78 1158.0 79 850.0 80 542.0 81 389.0 82 236.0 83 178.0 84 120.0 85 74.5 86 29.0 87 25.0 88 21.0 89 17.5 90 14.0 91 8.0 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1131434.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.124987882485204 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55524754580946 20.840457176999298 2 6.66322283565066 3.8813256869324833 3 3.219462917228872 2.813004553572041 4 2.1704576068682 2.5285820599793665 5 1.6549908262898174 2.4100793880657547 6 1.348056595240833 2.3557279200776113 7 1.22217147048965 2.491701048783112 8 1.0928930232969332 2.5464396848300637 9 0.9759239925578503 2.558139701172658 >10 9.994031608336055 54.53358915448059 >50 0.08179221260208701 1.4808822337344116 >100 0.020524049256645596 1.1200466801444844 >500 6.126581864274584E-4 0.12856358655307565 >1k 6.126581864274584E-4 0.3114611246750778 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2194 0.1939132110224724 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1297 0.11463328837563658 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03614881645769882 0.0 2 0.0 0.0 0.0 0.10782776547284242 0.0 3 0.0 0.0 0.0 0.15458259164918148 0.0 4 0.0 0.0 0.0 0.266917911252446 0.0 5 0.0 0.0 0.0 0.45473266668670026 0.0 6 0.0 0.0 0.0 0.7754760772612455 0.0 7 0.0 0.0 0.0 0.932710171340087 0.0 8 0.0 0.0 0.0 1.3553596586279004 0.0 9 0.0 0.0 0.0 1.6353583152000029 0.0 10 0.0 0.0 0.0 2.0963662043035654 0.0 11 0.0 0.0 0.0 2.5056697960287564 0.0 12 0.0 0.0 0.0 2.85195601334236 0.0 13 0.0 0.0 0.0 3.014051195208912 0.0 14 0.0 0.0 0.0 3.0813109735079554 0.0 15 0.0 0.0 0.0 3.166247434671399 0.0 16 0.0 0.0 0.0 3.334617838954813 0.0 17 0.0 0.0 0.0 3.5267633816908455 0.0 18 0.0 0.0 0.0 3.7826333661530414 0.0 19 0.0 0.0 0.0 3.9168877725081623 0.0 20 0.0 0.0 0.0 4.060952737853025 0.0 21 0.0 0.0 0.0 4.2492094103588895 0.0 22 0.0 0.0 0.0 4.444890289667802 0.0 23 0.0 0.0 0.0 4.670091229360263 0.0 24 0.0 0.0 0.0 4.844913622889183 0.0 25 0.0 0.0 0.0 4.996491178451416 0.0 26 0.0 0.0 0.0 5.140644527210601 0.0 27 0.0 0.0 0.0 5.2771085189237725 0.0 28 0.0 0.0 0.0 5.419405815982196 0.0 29 0.0 0.0 0.0 5.58998580562366 0.0 30 0.0 0.0 0.0 5.792737358078332 0.0 31 0.0 0.0 0.0 5.966852684292676 0.0 32 0.0 0.0 0.0 6.152192704125914 0.0 33 0.0 0.0 0.0 6.3315226517852565 0.0 34 8.838341432200198E-5 0.0 0.0 6.507228879457396 0.0 35 8.838341432200198E-5 0.0 0.0 6.7209399752879975 0.0 36 8.838341432200198E-5 0.0 0.0 6.901242140504881 0.0 37 8.838341432200198E-5 0.0 0.0 7.086228626680832 0.0 38 8.838341432200198E-5 0.0 0.0 7.299409422025501 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 20 7.85643E-4 44.0 1 TCGATAG 80 0.0 44.0 1 ACTAACG 25 4.4427965E-5 44.0 24 GCGTAAT 20 7.85643E-4 44.0 36 TACGCGG 70 0.0 40.857143 2 CGGTCTA 140 0.0 40.857143 31 CGTTTTT 2745 0.0 40.553734 1 ATTACGA 55 7.8216544E-11 40.0 2 TCGTAAG 45 2.3508619E-8 39.11111 1 TGATTCG 85 0.0 38.82353 15 ACAACGA 145 0.0 37.931034 13 GAGCGAT 685 0.0 37.897808 7 GTACCGG 35 7.2901057E-6 37.714287 2 GCGTAAG 65 1.0913936E-11 37.230766 1 ACGGGCC 60 1.9826984E-10 36.666668 5 TCTACGA 30 1.3010822E-4 36.666668 2 CGTAAGG 90 0.0 36.666668 2 CGATACG 30 1.3010822E-4 36.666668 10 GTAGGGC 380 0.0 35.894737 4 ATAGGGC 450 0.0 35.68889 4 >>END_MODULE