FastQCFastQC Report
Thu 26 May 2016
SRR1547160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547160_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences393297
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.16603228603320136No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC207.850157E-444.0000045
TCGTAAG207.850157E-444.0000041
CGTAAGA207.850157E-444.0000042
AATCGAG207.850157E-444.0000041
CGAAAGG502.7284841E-1144.02
AGTAGCG302.523453E-644.01
GCGTATG254.4374832E-544.01
TAGGGCG1200.042.1666645
GAGCGAT3500.042.1142857
AGCGATA950.041.684218
CGACCTC557.8216544E-1140.012
CGTTTTT8050.039.900621
CGAATGG501.3424142E-939.62
CGACCCG452.3441316E-839.1111132
CCGATAT404.115318E-738.50000411
GCCGATT404.115318E-738.5000049
TAGTGCG404.115318E-738.5000041
GGACCGA1450.037.9310348
ATTCGAT357.277995E-637.71428714
ACGAGAT357.277995E-637.7142875
Produced by FastQC (version 0.11.5)