##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547160_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	393297
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486530535447766	31.0	31.0	33.0	30.0	34.0
2	31.885061416690185	31.0	31.0	34.0	30.0	34.0
3	32.04060036054178	33.0	31.0	34.0	30.0	34.0
4	35.73553065495033	37.0	35.0	37.0	35.0	37.0
5	35.63729446194606	37.0	35.0	37.0	33.0	37.0
6	35.68972049113011	37.0	35.0	37.0	33.0	37.0
7	35.98795820969903	37.0	35.0	37.0	35.0	37.0
8	22.06831224240205	35.0	0.0	37.0	0.0	37.0
9	29.957146888992288	37.0	18.0	39.0	17.0	39.0
10	34.34672270574146	35.0	32.0	39.0	27.0	39.0
11	35.961166751844026	37.0	35.0	39.0	32.0	39.0
12	36.42669788988983	37.0	35.0	39.0	32.0	39.0
13	36.572976656318254	39.0	35.0	39.0	33.0	39.0
14	37.66548689667096	39.0	36.0	41.0	33.0	41.0
15	37.889104163011666	40.0	36.0	41.0	33.0	41.0
16	37.94289557255713	40.0	36.0	41.0	33.0	41.0
17	37.88384859279374	40.0	36.0	41.0	33.0	41.0
18	37.85101844153401	40.0	36.0	41.0	33.0	41.0
19	37.88583945466149	40.0	36.0	41.0	33.0	41.0
20	37.81040282534573	40.0	36.0	41.0	33.0	41.0
21	37.69469383188786	40.0	36.0	41.0	33.0	41.0
22	37.66071187931767	40.0	36.0	41.0	33.0	41.0
23	37.52736735851023	39.0	35.0	41.0	32.0	41.0
24	37.45644131534184	39.0	35.0	41.0	32.0	41.0
25	37.28891143334427	39.0	35.0	41.0	32.0	41.0
26	37.23092217840462	39.0	35.0	41.0	32.0	41.0
27	37.14055535638462	39.0	35.0	41.0	32.0	41.0
28	37.164753354335275	39.0	35.0	41.0	32.0	41.0
29	37.06750877835326	39.0	35.0	41.0	32.0	41.0
30	36.98178221547584	39.0	35.0	41.0	31.0	41.0
31	36.94730445439452	39.0	35.0	41.0	31.0	41.0
32	36.8115292005787	39.0	35.0	41.0	31.0	41.0
33	36.75264749031902	39.0	35.0	41.0	31.0	41.0
34	36.690465475200675	39.0	35.0	41.0	31.0	41.0
35	36.56641418571716	39.0	35.0	41.0	30.0	41.0
36	36.452042603935446	39.0	35.0	41.0	30.0	41.0
37	36.43686323567177	39.0	35.0	41.0	30.0	41.0
38	36.32506731554017	39.0	35.0	41.0	30.0	41.0
39	36.210708446797206	39.0	35.0	41.0	30.0	41.0
40	36.1394315237594	39.0	35.0	40.0	30.0	41.0
41	36.11530471882572	39.0	35.0	40.0	30.0	41.0
42	35.86565623434707	38.0	35.0	40.0	29.0	41.0
43	35.90968148752724	38.0	35.0	40.0	29.0	41.0
44	35.72457455815834	38.0	35.0	40.0	29.0	41.0
45	35.51404409390359	38.0	34.0	40.0	28.0	41.0
46	35.568672021398584	38.0	34.0	40.0	28.0	41.0
47	35.436080112484966	38.0	34.0	40.0	28.0	41.0
48	35.429698167034076	38.0	34.0	40.0	28.0	41.0
49	35.331029730712416	38.0	34.0	40.0	27.0	41.0
50	34.892793486855986	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	5.0
16	18.0
17	51.0
18	120.0
19	235.0
20	416.0
21	724.0
22	1060.0
23	1591.0
24	2298.0
25	3138.0
26	4086.0
27	5239.0
28	5977.0
29	7010.0
30	9099.0
31	11528.0
32	14644.0
33	19718.0
34	29387.0
35	42478.0
36	40464.0
37	60546.0
38	67820.0
39	65642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.31759967658029	23.570990879666002	26.760692301238	12.350717142515707
2	32.80091127061737	24.758388698617075	28.090984675703094	14.34971535506246
3	30.96794534410382	25.8486589015426	28.790201806777066	14.393193947576513
4	26.509482655601236	28.85783517291004	28.669173677907533	15.963508493581186
5	24.007048108681225	32.43299592928499	27.754851931237717	15.805104030796066
6	21.996099639712483	41.3000861944027	25.73729268212064	10.96652148376418
7	88.4024032728447	4.071986310599877	5.614332171361592	1.9112782451938357
8	54.43850321767011	40.282534573108876	3.4073486449172	1.8716135643038212
9	83.76900917118616	5.009700048563808	7.606719603760008	3.6145711764900312
10	43.16712306475768	27.933597256017716	14.77433084920556	14.124948830019044
11	34.894240230665375	26.886551384831307	22.1827270485155	16.036481335987816
12	31.755645224855517	24.118160067328255	26.18733425375734	17.93886045405889
13	24.328433728200316	30.523751770290648	27.469317080984602	17.67849742052444
14	20.40849536101216	33.096616551867925	28.611202221222133	17.88368586589778
15	19.599437575165844	25.909427226752303	36.54540970309969	17.945725494982163
16	22.193914522612683	24.012641845729817	34.799146700839316	18.994296930818187
17	22.623869493029442	23.74668507514677	27.216073349148353	26.413372082675433
18	24.7606770455915	25.02815938082416	29.615278021444354	20.595885552139986
19	28.073949203782384	27.072670272084455	25.13749151404664	19.715889010086524
20	30.535956287487576	25.714917733926267	24.31546642867858	19.433659549907574
21	26.64373234476744	27.175900146708464	26.20945494117677	19.970912567347323
22	25.576091350811218	24.51709522320282	26.460664586813525	23.44614883917243
23	22.801343513934764	29.493995631799883	25.84281090371907	21.86184995054628
24	23.1046766184334	26.500075006928608	30.753857771607716	19.641390603030278
25	22.790918822162386	26.31522742355014	29.142098719288477	21.751755034998997
26	21.840746306226592	30.550194890883986	26.377521313409456	21.23153748947996
27	21.819642661906904	28.718500268245116	27.873845973907756	21.588011095940217
28	20.376204242595293	28.544585898188902	31.171099703277676	19.908110155938132
29	21.220350015382778	26.281410740483658	31.03837557876109	21.45986366537248
30	22.586238898338916	27.125810774046077	29.172101490730924	21.115848836884084
31	26.2859874344325	26.470326496261094	25.95188877616661	21.29179729313979
32	26.177418083534835	27.319048962997428	25.523459370399472	20.98007358306827
33	24.739319140496878	27.05716036481336	26.3014973417036	21.902023152986168
34	21.832101439878766	27.93791968919163	27.88783031652924	22.342148554400364
35	21.58775683516528	27.363290337836293	28.481020704454902	22.567932122543525
36	26.263866747013072	27.130387467994925	26.370656272486187	20.235089512505816
37	23.365548173517723	28.840291179439458	26.78128742400781	21.012873223035008
38	22.945763634098405	29.530863444165607	25.45887713356573	22.064495788170262
39	23.00373509078381	27.818417124971713	26.28853004218186	22.889317742062616
40	24.757625916292266	26.24632275354249	26.74823352326613	22.24781780689911
41	21.721498002781615	25.900273838854602	27.313455225948836	25.064772932414943
42	23.56666844649209	26.987238651705965	26.036049092670428	23.410043809131523
43	23.39580520573511	26.836716272943857	26.603559142327555	23.163919378993484
44	22.68870599063812	28.421015161570008	26.923164936422094	21.96711391136978
45	21.84481447862557	29.156337322684895	25.250637558893153	23.748210639796387
46	23.370124867466572	28.0945443265522	26.887314167156116	21.648016638825112
47	22.578102553540962	27.655944489787615	27.447959175889974	22.317993780781446
48	23.404958593632802	26.25242501214095	27.713915946473023	22.628700447753225
49	22.524199269254535	25.590329954207636	28.58959005535257	23.29588072118526
50	20.915745607009463	28.82096736054432	27.758157321311884	22.505129711134334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	413.5
2	607.0
3	1325.0
4	2043.0
5	1547.0
6	1051.0
7	1107.0
8	1163.0
9	1263.0
10	1363.0
11	1395.5
12	1428.0
13	1439.5
14	1451.0
15	1402.5
16	1354.0
17	1381.0
18	1408.0
19	1518.5
20	1629.0
21	1711.0
22	1793.0
23	2054.0
24	2315.0
25	2648.0
26	2981.0
27	3539.5
28	4098.0
29	4928.5
30	5759.0
31	6793.5
32	7828.0
33	8842.5
34	9857.0
35	10921.0
36	11985.0
37	13453.5
38	14922.0
39	17455.0
40	19988.0
41	22930.5
42	25873.0
43	27454.0
44	29035.0
45	29564.5
46	30094.0
47	30537.0
48	30980.0
49	32137.5
50	33295.0
51	31925.0
52	30555.0
53	27958.5
54	25362.0
55	23237.5
56	21113.0
57	19602.0
58	18091.0
59	16737.0
60	15383.0
61	13832.0
62	12281.0
63	10482.5
64	8684.0
65	7279.5
66	5875.0
67	5074.0
68	4273.0
69	3532.0
70	2791.0
71	2305.5
72	1820.0
73	1489.0
74	1158.0
75	906.5
76	655.0
77	548.5
78	442.0
79	308.5
80	175.0
81	125.0
82	75.0
83	48.5
84	22.0
85	19.0
86	16.0
87	10.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	393297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.08035203335818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.87055423494795	24.322131898325143
2	8.633225252406165	6.575125135898086
3	5.713571366613823	6.527244270251092
4	4.502499810274588	6.858271112215389
5	3.7509665376213612	7.1419063108984036
6	2.9830394856628013	6.8157116246068306
7	2.4606869908130964	6.559267879784726
8	1.8671061049103426	5.688004620689442
9	1.5241568934405338	5.223638795065661
>10	4.668638956613671	23.208792035751763
>50	0.019502016688802432	0.5062781094965638
>100	0.005379866672773085	0.4064053413105953
>500	6.724833340966356E-4	0.16722286570624997
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	653	0.16603228603320136	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028731467567766854	0.0
2	0.0	0.0	0.0	0.09992448454984401	0.0
3	0.0	0.0	0.0	0.14670846713806615	0.0
4	0.0	0.0	0.0	0.24688721246284615	0.0
5	0.0	0.0	0.0	0.4106311515216236	0.0
6	0.0	0.0	0.0	0.7236261654678271	0.0
7	0.0	0.0	0.0	0.8634695916826215	0.0
8	0.0	0.0	0.0	1.2135866787694796	0.0
9	0.0	0.0	0.0	1.4439469408614864	0.0
10	0.0	0.0	0.0	1.804234458945784	0.0
11	0.0	0.0	0.0	2.16019954385617	0.0
12	0.0	0.0	0.0	2.4426832648100545	0.0
13	0.0	0.0	0.0	2.583289473349657	0.0
14	0.0	0.0	0.0	2.6460918847588464	0.0
15	0.0	0.0	0.0	2.7226243780145793	0.0
16	0.0	0.0	0.0	2.8614507611296296	0.0
17	0.0	0.0	0.0	3.0157870515157756	0.0
18	0.0	0.0	0.0	3.2138561951909117	0.0
19	0.0	0.0	0.0	3.327765022362235	0.0
20	0.0	0.0	0.0	3.437097155584711	0.0
21	0.0	0.0	0.0	3.587365273571881	0.0
22	0.0	0.0	0.0	3.7437356501575145	0.0
23	0.0	0.0	0.0	3.912310543940076	0.0
24	0.0	0.0	0.0	4.059019011078142	0.0
25	0.0	0.0	0.0	4.182844008471969	0.0
26	0.0	0.0	0.0	4.297261357193165	0.0
27	0.0	0.0	0.0	4.398457145617688	0.0
28	0.0	0.0	0.0	4.519993796037092	0.0
29	0.0	0.0	0.0	4.648141226604831	0.0
30	0.0	0.0	0.0	4.814427773412968	0.0
31	0.0	0.0	0.0	4.956051025052314	0.0
32	0.0	0.0	0.0	5.115726791712116	0.0
33	0.0	0.0	0.0	5.273114211397494	0.0
34	0.0	0.0	0.0	5.428467544883383	0.0
35	0.0	0.0	0.0	5.601619132614792	0.0
36	0.0	0.0	0.0	5.747056295878179	0.0
37	0.0	0.0	0.0	5.903172411688876	0.0
38	0.0	0.0	0.0	6.071238783921566	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAC	20	7.850157E-4	44.000004	5
TCGTAAG	20	7.850157E-4	44.000004	1
CGTAAGA	20	7.850157E-4	44.000004	2
AATCGAG	20	7.850157E-4	44.000004	1
CGAAAGG	50	2.7284841E-11	44.0	2
AGTAGCG	30	2.523453E-6	44.0	1
GCGTATG	25	4.4374832E-5	44.0	1
TAGGGCG	120	0.0	42.166664	5
GAGCGAT	350	0.0	42.114285	7
AGCGATA	95	0.0	41.68421	8
CGACCTC	55	7.8216544E-11	40.0	12
CGTTTTT	805	0.0	39.90062	1
CGAATGG	50	1.3424142E-9	39.6	2
CGACCCG	45	2.3441316E-8	39.11111	32
CCGATAT	40	4.115318E-7	38.500004	11
GCCGATT	40	4.115318E-7	38.500004	9
TAGTGCG	40	4.115318E-7	38.500004	1
GGACCGA	145	0.0	37.931034	8
ATTCGAT	35	7.277995E-6	37.714287	14
ACGAGAT	35	7.277995E-6	37.714287	5
>>END_MODULE