FastQCFastQC Report
Thu 26 May 2016
SRR1547159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547159_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences943198
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.15871534926918845No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11290.11969915118564714No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC207.8557624E-444.013
TCGCATC207.8557624E-444.032
TCGATAG302.5272311E-644.01
TAGCCGT302.5272311E-644.044
TGTTACG254.4422308E-544.01
CGAATAT700.044.014
ACGTAGT207.8557624E-444.011
CAATACG254.4422308E-544.027
GAGCGAT6150.040.4227647
GCGCGAC1800.040.3333329
CGGTCTA603.6379788E-1240.33333231
TCTAGCG557.8216544E-1140.0000041
TATTGCG501.3478711E-939.61
CGTTTTT18250.039.539731
ATAGCGG750.038.133332
CGCAGTA1500.038.1333337
CTAAGCG357.288816E-637.7142871
TAACGCC357.288816E-637.71428712
CTTGCGA357.288816E-637.7142872
CGTAAGA357.288816E-637.7142872
Produced by FastQC (version 0.11.5)