##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547159_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 943198 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49738443041652 31.0 31.0 33.0 30.0 34.0 2 31.89563060990375 31.0 31.0 34.0 30.0 34.0 3 32.064356582605136 33.0 31.0 34.0 30.0 34.0 4 35.75338370098325 37.0 35.0 37.0 35.0 37.0 5 35.64357748850188 37.0 35.0 37.0 33.0 37.0 6 35.69256720222053 37.0 35.0 37.0 33.0 37.0 7 35.99849766432923 37.0 35.0 37.0 35.0 37.0 8 22.070358503728805 35.0 0.0 37.0 0.0 37.0 9 29.981527738608435 37.0 18.0 39.0 17.0 39.0 10 34.32947482925112 35.0 32.0 39.0 27.0 39.0 11 35.96623402509335 37.0 35.0 39.0 32.0 39.0 12 36.38161128416303 37.0 35.0 39.0 32.0 39.0 13 36.497525440045465 39.0 35.0 39.0 32.0 39.0 14 37.564544242036135 39.0 36.0 41.0 33.0 41.0 15 37.82488618508521 40.0 36.0 41.0 33.0 41.0 16 37.89566877792362 40.0 36.0 41.0 33.0 41.0 17 37.84746044838941 40.0 36.0 41.0 33.0 41.0 18 37.818223745173334 40.0 36.0 41.0 33.0 41.0 19 37.862033210418176 40.0 36.0 41.0 33.0 41.0 20 37.79119018488165 40.0 36.0 41.0 33.0 41.0 21 37.68314712287346 40.0 36.0 41.0 33.0 41.0 22 37.66287884410272 40.0 35.0 41.0 33.0 41.0 23 37.54841401275236 39.0 35.0 41.0 33.0 41.0 24 37.454085992548755 39.0 35.0 41.0 32.0 41.0 25 37.31413870682508 39.0 35.0 41.0 32.0 41.0 26 37.2796273953083 39.0 35.0 41.0 32.0 41.0 27 37.17203068708797 39.0 35.0 41.0 32.0 41.0 28 37.19215265511589 39.0 35.0 41.0 32.0 41.0 29 37.1161113573184 39.0 35.0 41.0 32.0 41.0 30 37.018149953668264 39.0 35.0 41.0 31.0 41.0 31 36.98070076484471 39.0 35.0 41.0 31.0 41.0 32 36.86179678073957 39.0 35.0 41.0 31.0 41.0 33 36.80714017629384 39.0 35.0 41.0 31.0 41.0 34 36.75461249917833 39.0 35.0 41.0 31.0 41.0 35 36.64843860992072 39.0 35.0 41.0 31.0 41.0 36 36.542947504129565 39.0 35.0 41.0 30.0 41.0 37 36.515243883044704 39.0 35.0 41.0 30.0 41.0 38 36.410043278293635 39.0 35.0 41.0 30.0 41.0 39 36.33817501733464 39.0 35.0 41.0 30.0 41.0 40 36.27395520346735 39.0 35.0 41.0 30.0 41.0 41 36.276594097951865 39.0 35.0 41.0 30.0 41.0 42 36.045820707847135 38.0 35.0 40.0 29.0 41.0 43 36.09392195488116 38.0 35.0 40.0 30.0 41.0 44 35.92591905411165 38.0 35.0 40.0 29.0 41.0 45 35.72049347008794 38.0 35.0 40.0 29.0 41.0 46 35.78322367095774 38.0 35.0 40.0 29.0 41.0 47 35.64189597518231 38.0 35.0 40.0 29.0 41.0 48 35.643237156991425 38.0 35.0 40.0 29.0 41.0 49 35.56022383423205 38.0 34.0 40.0 28.0 41.0 50 35.112251086198235 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 3.0 14 7.0 15 12.0 16 43.0 17 87.0 18 229.0 19 430.0 20 905.0 21 1466.0 22 2352.0 23 3460.0 24 5050.0 25 6992.0 26 9649.0 27 11843.0 28 13857.0 29 16795.0 30 20902.0 31 27211.0 32 34928.0 33 46395.0 34 72665.0 35 106445.0 36 94216.0 37 142234.0 38 164393.0 39 160626.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.2877126541829 23.45138560514335 28.941007084408575 12.319894656265175 2 30.785900733462114 25.06780124639789 30.068129915457835 14.078168104682156 3 29.982357893040483 25.34547359091092 29.688252095530316 14.983916420518279 4 25.936229720588887 28.355234001768455 29.541093174497824 16.167443103144834 5 23.750368427413967 32.63853400876592 28.244864810994088 15.366232752826022 6 20.91649897476458 41.16982860438635 26.582541523624943 11.331130897224124 7 88.42554797614075 3.981560605514431 5.5714706774187395 2.0214207409260836 8 54.90087977285787 40.07249803328675 3.2803292627846967 1.7462929310706765 9 85.10302184695048 4.352108465030672 7.202305348399805 3.3425643396190403 10 43.66103405647594 25.502704628296495 15.428997941047374 15.407263374180182 11 36.76757160214505 25.487331398073366 21.51594893118942 16.229148068592174 12 34.76131204688729 23.100451866946283 24.598016535234382 17.540219550932044 13 24.05475838583203 31.56686082879735 25.776242103990892 18.60213868137973 14 19.339841687535387 33.912603716292864 27.50567749295482 19.24187710321693 15 19.02251701127441 25.466869098534982 37.42374347697938 18.086870413211223 16 21.625363921467176 23.292988322706368 35.91557658095119 19.166071174875267 17 21.8446179911323 23.359358268359347 27.07607522492626 27.719948515582093 18 24.262243982705648 24.51415291381025 30.398283287284322 20.82531981619978 19 26.56059491220295 27.02539657632862 25.6636464453911 20.750362066077326 20 29.00313613896552 25.95573781963066 25.08105403107301 19.96007201033081 21 24.772635226113714 27.778260768152602 27.43273416610298 20.016369839630705 22 24.473758426120497 24.054016229890223 26.989561046567108 24.482664297422176 23 22.791290906045177 28.732885353870557 25.864346616511064 22.611477123573206 24 22.251107402687452 26.590387172152614 31.154646214262545 20.003859210897392 25 21.444171849388994 26.901456534046936 29.479600253605287 22.174771362958783 26 20.6448699000634 30.570463465783433 26.77073106601159 22.013935568141576 27 21.701381894363646 29.426695137182225 27.87643739702586 20.99548557142827 28 18.839416538203007 28.9323132576617 31.523285672785565 20.704984531349726 29 20.36221450851253 26.163117394226877 31.10576994438071 22.368898152879883 30 21.86932118176671 27.63619091643536 30.269678264797 20.224809637000927 31 26.012247693485357 27.17308560874811 25.881310180895213 20.933356516871324 32 26.913118984561034 27.02210988572919 25.92149262403016 20.143278505679614 33 25.26712312791164 27.18527817064922 26.40813487730042 21.139463824138726 34 21.506194881668534 27.226520836558176 28.503241101020144 22.76404318075314 35 20.92625302428546 27.099824215064068 29.198217129383224 22.775705631267243 36 26.622829989037296 26.588372748881994 26.452452189254004 20.336345072826703 37 22.20170102141862 28.459771967285768 28.479492110882337 20.859034900413278 38 22.74220259160855 29.35841679053603 25.739028284623167 22.160352333232257 39 21.670529411639976 27.822366035551386 27.11551551211941 23.39158904068923 40 23.523798820608185 25.690682126128344 27.630253668900913 23.15526538436256 41 20.113592268007356 25.341868833479293 28.079258013693835 26.46528088481952 42 23.627700652461094 26.154741634312202 26.433686246154043 23.78387146707266 43 22.740930324279738 26.501434481413234 26.60607846920795 24.151556725099077 44 22.113914575730654 27.888523936649566 27.452666354254358 22.544895133365422 45 20.966859556530018 29.340817092487477 25.465384786651374 24.22693856433114 46 22.65759681424261 28.4296616405039 27.355125859045504 21.55761568620799 47 22.591756979976633 26.9434413558977 27.876543419303264 22.588258244822402 48 22.673500155852746 25.396258261785963 29.243912730943027 22.68632885141826 49 22.69226609895271 23.87399040286345 29.932527422662048 23.50121607552179 50 20.88129957866747 29.065477238077264 27.62431642136646 22.428906761888808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 398.0 1 768.5 2 1139.0 3 2966.5 4 4794.0 5 3585.5 6 2377.0 7 2519.0 8 2661.0 9 2767.5 10 2874.0 11 2980.5 12 3087.0 13 3011.5 14 2936.0 15 3008.0 16 3080.0 17 3042.5 18 3005.0 19 3090.0 20 3175.0 21 3430.5 22 3686.0 23 4150.0 24 4614.0 25 5530.5 26 6447.0 27 7858.5 28 9270.0 29 10930.5 30 12591.0 31 14253.0 32 15915.0 33 19052.5 34 22190.0 35 25391.0 36 28592.0 37 32413.0 38 36234.0 39 42715.5 40 49197.0 41 57602.5 42 66008.0 43 71493.0 44 76978.0 45 80713.0 46 84448.0 47 85251.0 48 86054.0 49 83587.5 50 81121.0 51 75360.0 52 69599.0 53 64051.0 54 58503.0 55 54132.5 56 49762.0 57 45699.5 58 41637.0 59 37174.0 60 32711.0 61 29357.0 62 26003.0 63 22543.5 64 19084.0 65 16048.0 66 13012.0 67 10388.0 68 7764.0 69 6209.5 70 4655.0 71 3913.0 72 3171.0 73 2689.5 74 2208.0 75 1723.0 76 1238.0 77 888.5 78 539.0 79 392.0 80 245.0 81 175.5 82 106.0 83 76.0 84 46.0 85 38.0 86 30.0 87 19.0 88 8.0 89 5.5 90 3.0 91 2.5 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 943198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.24271049711574 #Duplication Level Percentage of deduplicated Percentage of total 1 73.42640876874708 19.269079881611102 2 5.895559980518409 3.094309475742518 3 2.460330940553321 1.9369725780011178 4 1.6425752117928047 1.7242250301126856 5 1.3016665247063974 1.7079628885828373 6 1.1314781654963244 1.7815832358556591 7 1.0073952763051337 1.8505747814566282 8 0.8768689305784558 1.8409133991268718 9 0.8177048689299511 1.9312912932669606 >10 11.236214428754781 60.53256566294593 >50 0.18001376461212354 2.888772160239526 >100 0.022142922522110628 1.0003361996558202 >500 8.201082415596522E-4 0.15883135011746877 >1k 8.201082415596522E-4 0.2825820632848733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1497 0.15871534926918845 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1129 0.11969915118564714 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.046543779779007165 0.0 2 0.0 0.0 0.0 0.15659490372116988 0.0 3 0.0 0.0 0.0 0.22900811918600336 0.0 4 0.0 0.0 0.0 0.3637624337625822 0.0 5 0.0 0.0 0.0 0.6171556767507989 0.0 6 0.0 0.0 0.0 0.8945099544316252 0.0 7 0.0 0.0 0.0 1.036473783871467 0.0 8 0.0 0.0 0.0 1.4002362176340493 0.0 9 0.0 0.0 0.0 1.5996641214251939 0.0 10 0.0 0.0 0.0 1.9896140577058052 0.0 11 0.0 0.0 0.0 2.391862578164924 0.0 12 0.0 0.0 0.0 2.7055824969942686 0.0 13 0.0 0.0 0.0 2.8398067001838427 0.0 14 0.0 0.0 0.0 2.8867745690724536 0.0 15 0.0 0.0 0.0 2.964806965239536 0.0 16 0.0 0.0 0.0 3.1541627526775926 0.0 17 0.0 0.0 0.0 3.36535912926024 0.0 18 1.0602227740092749E-4 0.0 0.0 3.636034003464808 0.0 19 1.0602227740092749E-4 0.0 0.0 3.782026679445885 0.0 20 1.0602227740092749E-4 0.0 0.0 3.934380692071018 0.0 21 2.1204455480185497E-4 0.0 0.0 4.148439670143491 0.0 22 2.1204455480185497E-4 0.0 0.0 4.354016866023889 0.0 23 2.1204455480185497E-4 0.0 0.0 4.585463497590114 0.0 24 2.1204455480185497E-4 0.0 0.0 4.765171257784686 0.0 25 2.1204455480185497E-4 0.0 0.0 4.9154048248618 0.0 26 2.1204455480185497E-4 0.0 0.0 5.050159139438379 0.0 27 2.1204455480185497E-4 0.0 0.0 5.194773525813244 0.0 28 2.1204455480185497E-4 0.0 0.0 5.3442649369485515 0.0 29 2.1204455480185497E-4 0.0 0.0 5.516021026338054 0.0 30 2.1204455480185497E-4 0.0 0.0 5.727429447475504 0.0 31 2.1204455480185497E-4 0.0 0.0 5.909363675495495 0.0 32 2.1204455480185497E-4 0.0 0.0 6.075394561905347 0.0 33 2.1204455480185497E-4 0.0 0.0 6.243969982972822 0.0 34 2.1204455480185497E-4 0.0 0.0 6.413817671369108 0.0 35 3.180668322027825E-4 0.0 0.0 6.630315161821802 0.0 36 3.180668322027825E-4 0.0 0.0 6.822215483917481 0.0 37 3.180668322027825E-4 0.0 0.0 7.005528001543684 0.0 38 3.180668322027825E-4 0.0 0.0 7.223827870712194 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTAC 20 7.8557624E-4 44.0 13 TCGCATC 20 7.8557624E-4 44.0 32 TCGATAG 30 2.5272311E-6 44.0 1 TAGCCGT 30 2.5272311E-6 44.0 44 TGTTACG 25 4.4422308E-5 44.0 1 CGAATAT 70 0.0 44.0 14 ACGTAGT 20 7.8557624E-4 44.0 11 CAATACG 25 4.4422308E-5 44.0 27 GAGCGAT 615 0.0 40.422764 7 GCGCGAC 180 0.0 40.333332 9 CGGTCTA 60 3.6379788E-12 40.333332 31 TCTAGCG 55 7.8216544E-11 40.000004 1 TATTGCG 50 1.3478711E-9 39.6 1 CGTTTTT 1825 0.0 39.53973 1 ATAGCGG 75 0.0 38.13333 2 CGCAGTA 150 0.0 38.13333 37 CTAAGCG 35 7.288816E-6 37.714287 1 TAACGCC 35 7.288816E-6 37.714287 12 CTTGCGA 35 7.288816E-6 37.714287 2 CGTAAGA 35 7.288816E-6 37.714287 2 >>END_MODULE