FastQCFastQC Report
Thu 26 May 2016
SRR1547158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547158_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1658826
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.14823736787342373No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21060.12695725772323319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG600.044.01
ACGCTTA254.4436973E-544.026
CGTACCG254.4436973E-544.012
CGCTTAA254.4436973E-544.027
TCACGTA207.8574935E-444.012
TGCGTAG1050.041.9047621
TACGGGT1000.041.84
CGTAAGA850.041.4117662
TATAGCG850.041.4117661
CTTGCGA603.6379788E-1240.3333322
GAGCGAT9950.040.0201037
CGTTAGA1050.039.8095252
CGTTTTT30400.039.7302671
TACACGG850.038.8235322
TATGCGA1200.038.52
ATCGTAG800.038.51
CTATGCG1450.037.9310381
TTAGCGA2150.037.8604662
TAGAACG1050.037.7142871
ATTACCG700.037.7142871
Produced by FastQC (version 0.11.5)