##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547158_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1658826
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47936733569404	31.0	31.0	33.0	30.0	34.0
2	31.860574888505486	31.0	31.0	34.0	30.0	34.0
3	32.03404154504451	33.0	31.0	34.0	30.0	34.0
4	35.727559732003236	37.0	35.0	37.0	35.0	37.0
5	35.6254061607426	37.0	35.0	37.0	33.0	37.0
6	35.679646931022305	37.0	35.0	37.0	33.0	37.0
7	35.97557429169786	37.0	35.0	37.0	35.0	37.0
8	22.00615736671598	35.0	0.0	37.0	0.0	37.0
9	29.930561131788384	37.0	18.0	39.0	17.0	39.0
10	34.317522151208145	35.0	32.0	39.0	27.0	39.0
11	35.93817434739991	37.0	35.0	39.0	32.0	39.0
12	36.35207490116504	37.0	35.0	39.0	32.0	39.0
13	36.444330508443926	39.0	35.0	39.0	32.0	39.0
14	37.56459568393551	39.0	36.0	41.0	33.0	41.0
15	37.822880760248516	40.0	36.0	41.0	33.0	41.0
16	37.89595171524922	40.0	36.0	41.0	33.0	41.0
17	37.8410924352524	40.0	36.0	41.0	33.0	41.0
18	37.82898507739811	40.0	36.0	41.0	33.0	41.0
19	37.85069259825925	40.0	36.0	41.0	33.0	41.0
20	37.76479208789831	40.0	36.0	41.0	33.0	41.0
21	37.66619042624121	40.0	35.0	41.0	33.0	41.0
22	37.61904081561297	39.0	35.0	41.0	33.0	41.0
23	37.5185191213545	39.0	35.0	41.0	33.0	41.0
24	37.43140751350654	39.0	35.0	41.0	32.0	41.0
25	37.300015191466734	39.0	35.0	41.0	32.0	41.0
26	37.240508648887825	39.0	35.0	41.0	32.0	41.0
27	37.144727053952614	39.0	35.0	41.0	32.0	41.0
28	37.157627141122695	39.0	35.0	41.0	32.0	41.0
29	37.077184104903104	39.0	35.0	41.0	32.0	41.0
30	36.99759408159747	39.0	35.0	41.0	31.0	41.0
31	36.9640522875817	39.0	35.0	41.0	31.0	41.0
32	36.827003555526616	39.0	35.0	41.0	31.0	41.0
33	36.76521347024944	39.0	35.0	41.0	31.0	41.0
34	36.7349619550212	39.0	35.0	41.0	31.0	41.0
35	36.617305853657946	39.0	35.0	41.0	31.0	41.0
36	36.518509475978796	39.0	35.0	41.0	30.0	41.0
37	36.485252220546336	39.0	35.0	41.0	30.0	41.0
38	36.415408246555096	39.0	35.0	41.0	30.0	41.0
39	36.34407225350941	39.0	35.0	41.0	30.0	41.0
40	36.25675688709967	39.0	35.0	40.0	30.0	41.0
41	36.256497667627585	39.0	35.0	40.0	30.0	41.0
42	36.03096889004633	38.0	35.0	40.0	29.0	41.0
43	36.06766050206592	38.0	35.0	40.0	30.0	41.0
44	35.885193504321734	38.0	35.0	40.0	29.0	41.0
45	35.68673447365788	38.0	35.0	40.0	28.0	41.0
46	35.75351362951871	38.0	35.0	40.0	29.0	41.0
47	35.60406697266621	38.0	34.0	40.0	28.0	41.0
48	35.62408594994291	38.0	34.0	40.0	28.0	41.0
49	35.53523817446797	38.0	34.0	40.0	28.0	41.0
50	35.083061152887645	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	4.0
13	4.0
14	13.0
15	32.0
16	74.0
17	178.0
18	390.0
19	810.0
20	1455.0
21	2421.0
22	3943.0
23	5807.0
24	8436.0
25	12317.0
26	16547.0
27	20918.0
28	24507.0
29	29513.0
30	37612.0
31	48413.0
32	62996.0
33	83922.0
34	130828.0
35	192862.0
36	164920.0
37	246242.0
38	285368.0
39	278286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.85276575119995	23.25686961742823	28.72579764242904	12.164566988942783
2	30.374493768484456	25.624327084335548	29.80601943784339	14.195159709336602
3	30.53062828771673	25.881316063288136	29.691902586528062	13.89615306246707
4	27.07185684333378	28.456691660246463	28.843531509633923	15.627919986785836
5	24.053577650699953	32.56097987371792	27.826125223501442	15.559317252080687
6	21.594247980198045	42.36502200954169	25.228384411626052	10.812345598634215
7	88.89455554711586	4.157458347047852	5.131339875309406	1.816646230526891
8	55.10023353865927	40.40375542703092	2.910311268330735	1.585699765979072
9	85.47273794840447	5.023733652595269	6.555781016212671	2.9477473827875857
10	45.551914426226745	27.23209064724088	13.670692405351737	13.545302521180641
11	37.518461851936244	25.55198676654453	21.23495773516933	15.694593646349889
12	35.85083667605885	22.77966465440016	24.50757342843674	16.86192524110425
13	23.528808928724292	33.221507258748055	25.074118683936714	18.175565128590943
14	18.2550791945629	35.67167382232977	26.90053085736539	19.17271612574194
15	17.350885505773363	27.061126362861444	38.53092488301968	17.057063248345518
16	20.306952025106913	22.482225381082767	37.690390673886235	19.520431919924093
17	20.210317417257748	23.33969928129894	27.76722814809992	28.682755153343386
18	23.213766844744416	25.796979309463442	29.589963022040887	21.399290823751254
19	27.053832047484182	27.765721058145942	24.99472518516107	20.1857217092088
20	30.320961933319108	24.82140984045343	25.163579543604936	19.69404868262253
21	25.05151233462702	27.643104219490173	26.422843625552044	20.882539820330763
22	24.131403775923456	25.141515746678678	26.018039263913153	24.709041213484717
23	22.60984575838575	29.026431946448874	25.67659296393956	22.68712933122582
24	22.536058634238916	25.45493017350825	32.527884178328534	19.481127013924304
25	21.292649138607665	26.590854013621684	29.765207441889626	22.35128940588103
26	20.400331318655482	31.67059112890683	26.4747478035671	21.454329748870588
27	21.067851601072082	31.341020697770595	26.926814506162795	20.664313194994534
28	18.611415543281815	29.51213689681739	32.24648034212147	19.62996721777932
29	19.92180011646791	26.29630835301593	32.06695578680343	21.714935743712722
30	21.80252781183801	27.759632414731865	29.445945506038605	20.991894267391515
31	26.01641160676286	27.680540333947018	24.698853285395817	21.60419477389431
32	27.178799946468164	27.20713323760298	25.68189792057757	19.93216889535129
33	25.98837973361884	28.686191318438464	24.68149160912597	20.643937338816727
34	21.65061314447688	27.930777549905777	27.55551215136488	22.863097154252465
35	21.608294058569133	27.224193495882027	28.23563170579675	22.93188073975209
36	26.85543872594232	27.459540663095467	26.09930155423173	19.585719056730483
37	22.400842523567874	30.70888688747343	27.370742923006997	19.519527665951703
38	21.51804951212484	30.46708937525696	25.045363407614786	22.96949770500342
39	22.950689222377754	28.765403966419623	26.51821227783987	21.76569453336275
40	23.69000727020194	26.336818930978893	26.90927197909847	23.063901819720694
41	19.72780749759167	26.201120551522582	28.166185000717377	25.904886950168372
42	23.137447809474892	27.215452374148946	25.062122247903034	24.584977568473125
43	23.25536252747425	27.546107910052047	25.24351559476401	23.955013967709693
44	21.61866283745251	29.82386338289851	26.219446765362974	22.33802701428601
45	21.200776935013074	31.455499250674873	23.9310813792405	23.41264243507155
46	22.913072257126426	30.46208583660975	25.378912556229526	21.245929350034302
47	22.77604763851061	27.40920385863255	27.370441505016196	22.44430699784064
48	23.87272685622241	24.698973852592136	28.568095749644627	22.860203541540823
49	21.894279448236283	25.69684825292104	29.11010558069382	23.29876671814886
50	20.368742713220072	29.120353792380875	27.893220868252605	22.617682626146443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	530.0
1	1230.0
2	1930.0
3	4921.5
4	7913.0
5	6119.5
6	4326.0
7	4468.0
8	4610.0
9	4830.0
10	5050.0
11	5120.5
12	5191.0
13	5099.5
14	5008.0
15	4850.5
16	4693.0
17	4629.0
18	4565.0
19	4660.5
20	4756.0
21	5336.0
22	5916.0
23	7357.0
24	8798.0
25	11102.5
26	13407.0
27	16118.5
28	18830.0
29	22922.0
30	27014.0
31	30994.5
32	34975.0
33	39720.5
34	44466.0
35	48507.0
36	52548.0
37	58649.0
38	64750.0
39	77508.0
40	90266.0
41	104384.0
42	118502.0
43	127702.5
44	136903.0
45	137852.0
46	138801.0
47	137285.5
48	135770.0
49	135429.5
50	135089.0
51	127906.5
52	120724.0
53	110822.5
54	100921.0
55	93298.0
56	85675.0
57	79240.0
58	72805.0
59	68803.5
60	64802.0
61	58547.5
62	52293.0
63	42649.5
64	33006.0
65	26468.0
66	19930.0
67	16439.5
68	12949.0
69	10771.5
70	8594.0
71	7044.0
72	5494.0
73	4404.5
74	3315.0
75	2659.5
76	2004.0
77	1525.0
78	1046.0
79	713.5
80	381.0
81	280.0
82	179.0
83	109.5
84	40.0
85	34.0
86	28.0
87	25.5
88	23.0
89	14.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1658826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.697482189881264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54277008330799	16.049401027533246
2	5.916281563494316	2.449042645814929
3	1.997724274702527	1.2404358778784703
4	1.1547636730524997	0.9560280222610393
5	0.7912152729464096	0.8188082010085177
6	0.6155730071774916	0.7644486811576671
7	0.5117737615727096	0.7414699820779788
8	0.4610895664514	0.7634714471654339
9	0.40675255963697643	0.7576878472897443
>10	8.667320243996043	46.731774460424994
>50	1.813705987711082	24.504803080791714
>100	0.1177907600509018	3.5460914530201912
>500	0.0023558151998050855	0.3068187959623345
>1k	8.834306999269071E-4	0.3697184776137309
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2459	0.14823736787342373	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2106	0.12695725772323319	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034482218147050984	0.0
2	0.0	0.0	0.0	0.12056719631836009	0.0
3	0.0	0.0	0.0	0.1668047161064512	0.0
4	0.0	0.0	0.0	0.2734464012500407	0.0
5	0.0	0.0	0.0	0.45327237455887476	0.0
6	0.0	0.0	0.0	0.6637826993307315	0.0
7	0.0	0.0	0.0	0.7657825474160641	0.0
8	0.0	0.0	0.0	1.0590622524604751	0.0
9	0.0	0.0	0.0	1.206998202343103	0.0
10	0.0	0.0	0.0	1.5084161931390032	0.0
11	0.0	0.0	0.0	1.8444972528764318	0.0
12	0.0	0.0	0.0	2.1141457874424443	0.0
13	6.028359815918004E-5	0.0	0.0	2.221872577352899	0.0
14	6.028359815918004E-5	0.0	0.0	2.2622023045213906	0.0
15	6.028359815918004E-5	0.0	0.0	2.3349646074995207	0.0
16	6.028359815918004E-5	0.0	0.0	2.5038792495415434	0.0
17	6.028359815918004E-5	0.0	0.0	2.6982335700067397	0.0
18	6.028359815918004E-5	0.0	0.0	2.97318706121076	0.0
19	6.028359815918004E-5	0.0	0.0	3.097793258605785	0.0
20	6.028359815918004E-5	0.0	0.0	3.227704412638818	0.0
21	6.028359815918004E-5	0.0	0.0	3.414824701324913	0.0
22	6.028359815918004E-5	0.0	0.0	3.6026081095907587	0.0
23	6.028359815918004E-5	0.0	0.0	3.809561702071224	0.0
24	6.028359815918004E-5	0.0	0.0	3.9658167884998186	0.0
25	6.028359815918004E-5	0.0	0.0	4.094944255756782	0.0
26	6.028359815918004E-5	0.0	0.0	4.224132006611905	0.0
27	6.028359815918004E-5	0.0	0.0	4.348135368025338	0.0
28	6.028359815918004E-5	0.0	0.0	4.487269912576726	0.0
29	6.028359815918004E-5	0.0	0.0	4.625500203155726	0.0
30	6.028359815918004E-5	0.0	0.0	4.7999006526302335	0.0
31	6.028359815918004E-5	0.0	0.0	4.968875578270415	0.0
32	6.028359815918004E-5	0.0	0.0	5.119885991659161	0.0
33	6.028359815918004E-5	0.0	0.0	5.271499241029499	0.0
34	6.028359815918004E-5	0.0	0.0	5.430768507366053	0.0
35	6.028359815918004E-5	0.0	0.0	5.622651200306723	0.0
36	6.028359815918004E-5	0.0	0.0	5.793314066695362	0.0
37	6.028359815918004E-5	0.0	0.0	5.970366994488874	0.0
38	6.028359815918004E-5	0.0	0.0	6.14850502704925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	60	0.0	44.0	1
ACGCTTA	25	4.4436973E-5	44.0	26
CGTACCG	25	4.4436973E-5	44.0	12
CGCTTAA	25	4.4436973E-5	44.0	27
TCACGTA	20	7.8574935E-4	44.0	12
TGCGTAG	105	0.0	41.904762	1
TACGGGT	100	0.0	41.8	4
CGTAAGA	85	0.0	41.411766	2
TATAGCG	85	0.0	41.411766	1
CTTGCGA	60	3.6379788E-12	40.333332	2
GAGCGAT	995	0.0	40.020103	7
CGTTAGA	105	0.0	39.809525	2
CGTTTTT	3040	0.0	39.730267	1
TACACGG	85	0.0	38.823532	2
TATGCGA	120	0.0	38.5	2
ATCGTAG	80	0.0	38.5	1
CTATGCG	145	0.0	37.931038	1
TTAGCGA	215	0.0	37.860466	2
TAGAACG	105	0.0	37.714287	1
ATTACCG	70	0.0	37.714287	1
>>END_MODULE