FastQCFastQC Report
Thu 26 May 2016
SRR1547157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547157_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1560221
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37110.2378509198376384No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.1492096311996826No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT254.443575E-544.044
CGAACGC207.857348E-444.037
CTCGTTA207.857348E-444.032
CGTATCG207.857348E-444.013
CAAGTCG254.443575E-544.01
TTCGTTC302.5283025E-644.014
CAATTCG207.857348E-444.01
TCGATTG502.7284841E-1144.01
TCGATTC207.857348E-444.011
GCGTTGT207.857348E-444.029
ACTTACG254.443575E-544.024
TCGATAC254.443575E-544.024
CGCCTAT207.857348E-444.026
CCCGTTC254.443575E-544.014
CCGTAAG207.857348E-444.029
GTTCGAC207.857348E-444.030
CGTAGGA408.3164196E-944.02
CTACGCG207.857348E-444.01
TACGACC408.3164196E-944.010
CGCTAGT254.443575E-544.043
Produced by FastQC (version 0.11.5)