##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547157_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1560221
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519871864306403	31.0	31.0	33.0	30.0	34.0
2	31.908352086018585	31.0	31.0	34.0	30.0	34.0
3	32.061020842560126	33.0	31.0	34.0	30.0	34.0
4	35.745482851467834	37.0	35.0	37.0	35.0	37.0
5	35.6411040487213	37.0	35.0	37.0	33.0	37.0
6	35.68485105635676	37.0	35.0	37.0	33.0	37.0
7	36.021727691141194	37.0	35.0	37.0	35.0	37.0
8	22.067916660524375	35.0	0.0	37.0	0.0	37.0
9	29.991487103429577	37.0	18.0	39.0	17.0	39.0
10	34.458781159848506	35.0	32.0	39.0	27.0	39.0
11	35.968653799686074	37.0	35.0	39.0	32.0	39.0
12	36.18731513035653	37.0	35.0	39.0	32.0	39.0
13	36.16704364317619	38.0	35.0	39.0	32.0	39.0
14	37.254620339041715	39.0	35.0	41.0	32.0	41.0
15	37.58650216860304	39.0	35.0	41.0	33.0	41.0
16	37.691796226303836	39.0	35.0	41.0	33.0	41.0
17	37.66066409822711	39.0	35.0	41.0	33.0	41.0
18	37.60958159132584	39.0	36.0	41.0	33.0	41.0
19	37.64442216839794	39.0	36.0	41.0	33.0	41.0
20	37.56281513965009	39.0	35.0	41.0	33.0	41.0
21	37.411835887351856	39.0	35.0	41.0	33.0	41.0
22	37.388161677095745	39.0	35.0	41.0	33.0	41.0
23	37.291576642026996	39.0	35.0	41.0	32.0	41.0
24	37.217280116086116	39.0	35.0	41.0	32.0	41.0
25	37.09003339911461	39.0	35.0	41.0	32.0	41.0
26	37.0258174963675	39.0	35.0	41.0	32.0	41.0
27	36.89045782616694	39.0	35.0	41.0	32.0	41.0
28	36.95458015242713	39.0	35.0	41.0	32.0	41.0
29	36.89993981621834	39.0	35.0	41.0	32.0	41.0
30	36.82220916139444	38.0	35.0	40.0	31.0	41.0
31	36.75202038685545	38.0	35.0	40.0	31.0	41.0
32	36.576649077278155	38.0	35.0	41.0	31.0	41.0
33	36.52129986713421	38.0	35.0	41.0	31.0	41.0
34	36.485849761027445	39.0	35.0	41.0	31.0	41.0
35	36.37974812542582	38.0	35.0	41.0	30.0	41.0
36	36.2444365253384	38.0	35.0	41.0	30.0	41.0
37	36.18597557653691	38.0	35.0	40.0	30.0	41.0
38	36.12348827505847	38.0	35.0	40.0	30.0	41.0
39	36.06534394806889	38.0	35.0	40.0	30.0	41.0
40	35.99966479107768	38.0	35.0	40.0	30.0	41.0
41	36.029654773266095	38.0	35.0	40.0	30.0	41.0
42	35.79101422170321	38.0	35.0	40.0	29.0	41.0
43	35.83104380725551	38.0	35.0	40.0	30.0	41.0
44	35.6368905430705	38.0	35.0	40.0	29.0	41.0
45	35.45032210180481	38.0	34.0	40.0	28.0	41.0
46	35.52745540535604	38.0	34.0	40.0	29.0	41.0
47	35.389740299611404	38.0	34.0	40.0	28.0	41.0
48	35.44552342264333	38.0	34.0	40.0	28.0	41.0
49	35.38164144694886	37.0	34.0	40.0	28.0	41.0
50	34.89027900534604	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	5.0
14	10.0
15	33.0
16	63.0
17	148.0
18	414.0
19	881.0
20	1596.0
21	2689.0
22	4232.0
23	6112.0
24	9025.0
25	12628.0
26	16873.0
27	20625.0
28	24103.0
29	28992.0
30	35902.0
31	46245.0
32	59487.0
33	80586.0
34	131689.0
35	215251.0
36	145316.0
37	219018.0
38	260828.0
39	237467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.80513337533593	23.59454205526012	29.591512997197196	12.008811572206758
2	29.569016184245694	25.906970871434236	30.457736436056177	14.066276508263895
3	29.29982355063802	25.730521509452824	31.304924110110044	13.664730829799113
4	25.953310460505275	28.35649565029569	30.77256363040877	14.91763025879026
5	23.618320737895466	32.56833487050873	28.772590549672127	15.040753841923676
6	21.4203628844888	41.60865672234895	25.90947051731774	11.061509875844513
7	89.36855740308584	3.9405955951112057	5.122094882712128	1.5687521190908211
8	55.68864923622999	39.91633236573537	2.881707142770159	1.5133112552644785
9	86.48768347561018	4.705166768041194	6.3110290144793595	2.4961207418692606
10	52.997748395900324	26.543867823853155	10.041910729313347	10.416473050933169
11	42.50058164836904	20.606311541762352	22.045658916268913	14.84744789359969
12	39.44063052606009	22.700758418198447	22.905152539287705	14.953458516453757
13	21.304225491132346	40.088807931696856	24.306428384184038	14.300538192986762
14	15.244122467265855	42.15563051644607	27.22793758063761	15.372309435650463
15	14.896607595975187	23.86943900896091	46.457200614528325	14.77675278053558
16	17.011179826447666	20.552152547619855	44.5562519668688	17.880415659063683
17	18.478215586125298	20.523182292764936	26.90650875741321	34.09209336369655
18	21.951056933601073	23.945069320307827	32.695880904051414	21.407992842039686
19	28.283813639221624	26.37914756947894	24.450574630132525	20.886464161166913
20	30.53599458025498	24.861221583352613	24.33462951722865	20.26815431916376
21	23.246834903516874	28.226770438290472	27.548276814630746	20.978117843561904
22	23.312594818298177	24.451856499816373	26.02746662171577	26.20808206016968
23	20.52722018226905	31.187953501459088	24.401030366851874	23.883795949419987
24	20.79436182438257	24.988831710379493	35.6805862759186	18.536220189319334
25	17.837665305107418	26.796972992928563	32.94892197964263	22.41643972232139
26	17.91086006405503	34.66951156278502	26.247627739916336	21.172000633243623
27	20.00748611895366	32.86726688078163	28.00577610479541	19.119470895469295
28	16.240712052971983	29.322320363589515	36.33562168436394	18.101345899074552
29	17.018294203193012	25.15130869280698	36.32607175521929	21.504325348780718
30	19.215226560852596	29.806162075757214	31.018105768349486	19.960505595040704
31	28.694011938052366	26.654364990600694	23.787014788289607	20.864608283057336
32	28.8861001101767	27.708510525111507	24.361676967557802	19.043712397153993
33	26.16584445408695	27.69242306057924	24.888974062007883	21.252758423325925
34	19.99370601985232	28.44334232137627	28.23914048073959	23.323811178031832
35	19.909743555560397	26.335243532807212	30.083430488373118	23.67158242325927
36	29.530624187214507	24.630549133744513	27.12628531470862	18.71254136433236
37	21.757622798308702	29.277390831170713	28.925261229018197	20.03972514150239
38	20.876273297180337	32.56064365240565	23.5264106815637	23.03667236885031
39	21.46221592966637	30.373709878280064	27.275815413329262	20.888258778724296
40	24.15657781814243	25.905560814782007	25.172908196979787	24.764953170095776
41	18.530131308321067	24.08370352661578	27.339203869195455	30.046961295867703
42	23.515642976219393	26.210068958179644	24.41481046595322	25.85947759964774
43	23.220107920608683	27.201402878182	25.310901468445817	24.2675877327635
44	20.61124674004516	30.78903565584619	27.19358347311054	21.406134130998108
45	19.176578189884637	33.847256254081955	23.340539577406023	23.635625978627388
46	23.066347652031347	31.17436568281032	25.3076967942362	20.45158987092213
47	21.90401231620392	27.03354204308236	27.541418811822172	23.521026828891547
48	24.430513369580336	23.060707425422425	29.65265818111665	22.85612102388059
49	22.378432286195356	23.036223714460967	30.604510514856546	23.980833484487132
50	19.526656800542998	31.121680838804245	27.156729719699968	22.19493264095279
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	824.0
1	1235.5
2	1647.0
3	4336.0
4	7025.0
5	5311.5
6	3598.0
7	3984.0
8	4370.0
9	4821.5
10	5273.0
11	5596.0
12	5919.0
13	5686.5
14	5454.0
15	5310.5
16	5167.0
17	5066.0
18	4965.0
19	4945.0
20	4925.0
21	5258.0
22	5591.0
23	6800.5
24	8010.0
25	9482.0
26	10954.0
27	12270.0
28	13586.0
29	16715.5
30	19845.0
31	23695.5
32	27546.0
33	32044.0
34	36542.0
35	39648.0
36	42754.0
37	49493.5
38	56233.0
39	75059.0
40	93885.0
41	113989.5
42	134094.0
43	145150.0
44	156206.0
45	153774.5
46	151343.0
47	145153.5
48	138964.0
49	137323.0
50	135682.0
51	129577.0
52	123472.0
53	108618.5
54	93765.0
55	82315.0
56	70865.0
57	64039.5
58	57214.0
59	51789.0
60	46364.0
61	39584.5
62	32805.0
63	27783.0
64	22761.0
65	18302.5
66	13844.0
67	10369.0
68	6894.0
69	5774.0
70	4654.0
71	3837.5
72	3021.0
73	2510.5
74	2000.0
75	1643.5
76	1287.0
77	910.0
78	533.0
79	370.5
80	208.0
81	140.0
82	72.0
83	48.0
84	24.0
85	19.5
86	15.0
87	15.0
88	15.0
89	9.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1560221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.669671475581136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17805039547363	15.770759108315549
2	6.470447396872441	2.5454314919302012
3	1.947950987549197	1.1494666792687964
4	0.9776931816225396	0.7692361474572414
5	0.6498761487390563	0.6391425172756571
6	0.48900991815453665	0.5771198663040334
7	0.3785127685306987	0.5211655264418071
8	0.30095257513308554	0.4735710626078354
9	0.2731292483964705	0.48351263276978745
>10	5.1747544212849235	27.86756273261054
>50	2.681131951275303	36.72732830392424
>100	0.4755251536191057	11.749144509246733
>500	0.0019772355659829544	0.24417426550985694
>1k	9.886177829914772E-4	0.4823851563377635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3711	0.2378509198376384	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2328	0.1492096311996826	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032367209517113284	0.0
2	0.0	0.0	0.0	0.12677691173237637	0.0
3	0.0	0.0	0.0	0.18266642994806506	0.0
4	0.0	0.0	0.0	0.34789943219582353	0.0
5	0.0	0.0	0.0	0.5772900121200779	0.0
6	0.0	0.0	0.0	0.8862846994111732	0.0
7	0.0	0.0	0.0	1.0204964553098568	0.0
8	0.0	0.0	0.0	1.4229394425533306	0.0
9	0.0	0.0	0.0	1.6484203199418543	0.0
10	0.0	0.0	0.0	2.036185899305291	0.0
11	0.0	0.0	0.0	2.44349999134738	0.0
12	0.0	0.0	0.0	2.7648647210875894	0.0
13	0.0	0.0	0.0	2.892923502503812	0.0
14	0.0	0.0	0.0	2.938942624153886	0.0
15	0.0	0.0	0.0	3.0076508392080354	0.0
16	0.0	0.0	0.0	3.178459974580524	0.0
17	0.0	0.0	0.0	3.383046376122357	0.0
18	0.0	0.0	0.0	3.6862085563519527	0.0
19	0.0	0.0	0.0	3.816318329262329	0.0
20	0.0	0.0	0.0	3.9579649293273196	0.0
21	0.0	0.0	0.0	4.14242597683277	0.0
22	0.0	0.0	0.0	4.318683058361604	0.0
23	0.0	0.0	0.0	4.519936598725437	0.0
24	0.0	0.0	0.0	4.681452178890042	0.0
25	0.0	0.0	0.0	4.799896937677419	0.0
26	0.0	0.0	0.0	4.927378877735911	0.0
27	0.0	0.0	0.0	5.040375690366941	0.0
28	0.0	0.0	0.0	5.1568335511443575	0.0
29	0.0	0.0	0.0	5.2842513977186565	0.0
30	0.0	0.0	0.0	5.456662870195953	0.0
31	6.409348419230353E-5	0.0	0.0	5.6152301500877115	0.0
32	6.409348419230353E-5	0.0	0.0	5.7571972175736645	0.0
33	1.2818696838460706E-4	0.0	0.0	5.898459256733501	0.0
34	1.2818696838460706E-4	0.0	0.0	6.046835672638684	0.0
35	1.2818696838460706E-4	0.0	0.0	6.238090629468518	0.0
36	1.2818696838460706E-4	0.0	0.0	6.396529722391892	0.0
37	1.2818696838460706E-4	0.0	0.0	6.554327880473343	0.0
38	1.2818696838460706E-4	0.0	0.0	6.718599480458217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	25	4.443575E-5	44.0	44
CGAACGC	20	7.857348E-4	44.0	37
CTCGTTA	20	7.857348E-4	44.0	32
CGTATCG	20	7.857348E-4	44.0	13
CAAGTCG	25	4.443575E-5	44.0	1
TTCGTTC	30	2.5283025E-6	44.0	14
CAATTCG	20	7.857348E-4	44.0	1
TCGATTG	50	2.7284841E-11	44.0	1
TCGATTC	20	7.857348E-4	44.0	11
GCGTTGT	20	7.857348E-4	44.0	29
ACTTACG	25	4.443575E-5	44.0	24
TCGATAC	25	4.443575E-5	44.0	24
CGCCTAT	20	7.857348E-4	44.0	26
CCCGTTC	25	4.443575E-5	44.0	14
CCGTAAG	20	7.857348E-4	44.0	29
GTTCGAC	20	7.857348E-4	44.0	30
CGTAGGA	40	8.3164196E-9	44.0	2
CTACGCG	20	7.857348E-4	44.0	1
TACGACC	40	8.3164196E-9	44.0	10
CGCTAGT	25	4.443575E-5	44.0	43
>>END_MODULE