FastQCFastQC Report
Thu 26 May 2016
SRR1547156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547156_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences342885
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.46750368199250475No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.2872683261151698No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6160.17965206993598437No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA3590.10469982647243245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGT207.8487437E-444.0000047
ATATGCG207.8487437E-444.0000041
CGTTAGA207.8487437E-444.0000042
CGCTCCC207.8487437E-444.00000419
TATCTAG207.8487437E-444.0000041
AGCGATA650.044.0000048
GCGATAC408.283678E-944.0000049
ATGGTCG207.8487437E-444.00000444
TATACGA207.8487437E-444.0000042
ACGAGCC207.8487437E-444.0000045
CCGTTGG207.8487437E-444.0000041
ACCGCTC351.442113E-744.018
TGAACGC254.4362845E-544.029
AAGCGAC302.5225017E-644.015
CGCGACC900.044.010
TATGCGA254.4362845E-544.02
GTAGACG254.4362845E-544.01
CTACGGG302.5225017E-644.03
TCGCTAT302.5225017E-644.023
AGACCGA1000.044.08
Produced by FastQC (version 0.11.5)