##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547156_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	342885
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432419032620267	31.0	31.0	33.0	30.0	34.0
2	31.82441051664552	31.0	31.0	34.0	30.0	34.0
3	31.95270134301588	31.0	31.0	34.0	30.0	34.0
4	35.653111101389676	37.0	35.0	37.0	33.0	37.0
5	35.54447992767254	37.0	35.0	37.0	33.0	37.0
6	35.60184901643408	37.0	35.0	37.0	33.0	37.0
7	35.96818758475874	37.0	35.0	37.0	35.0	37.0
8	22.024078043658953	35.0	0.0	37.0	0.0	37.0
9	29.93620310016478	37.0	18.0	39.0	17.0	39.0
10	34.34545984805401	35.0	32.0	39.0	27.0	39.0
11	35.913740758563364	37.0	35.0	39.0	32.0	39.0
12	36.18915963078	37.0	35.0	39.0	32.0	39.0
13	36.21829184712075	38.0	35.0	39.0	32.0	39.0
14	37.29830701255523	39.0	35.0	41.0	32.0	41.0
15	37.61035624188868	39.0	35.0	41.0	33.0	41.0
16	37.69453023608499	39.0	35.0	41.0	33.0	41.0
17	37.667427854820126	39.0	35.0	41.0	33.0	41.0
18	37.62797731017688	39.0	36.0	41.0	33.0	41.0
19	37.62644326815113	39.0	36.0	41.0	33.0	41.0
20	37.50679382300188	39.0	35.0	41.0	33.0	41.0
21	37.40046371232337	39.0	35.0	41.0	33.0	41.0
22	37.37535325254823	39.0	35.0	41.0	33.0	41.0
23	37.2791169050848	39.0	35.0	41.0	32.0	41.0
24	37.143911223879726	39.0	35.0	41.0	32.0	41.0
25	36.98257724893186	38.0	35.0	41.0	32.0	41.0
26	36.883704449013514	38.0	35.0	41.0	32.0	41.0
27	36.72147221371597	38.0	35.0	40.0	31.0	41.0
28	36.75060151362702	38.0	35.0	40.0	31.0	41.0
29	36.676780844889684	38.0	35.0	40.0	31.0	41.0
30	36.58500663487758	38.0	35.0	40.0	31.0	41.0
31	36.496131355993995	38.0	35.0	40.0	31.0	41.0
32	36.239937588404274	38.0	35.0	40.0	30.0	41.0
33	36.10564183326771	38.0	35.0	40.0	30.0	41.0
34	36.0414191346953	38.0	35.0	40.0	30.0	41.0
35	35.884074835586276	38.0	35.0	40.0	30.0	41.0
36	35.72526357233475	38.0	35.0	40.0	29.0	41.0
37	35.64550213628476	38.0	35.0	40.0	29.0	41.0
38	35.5699403590125	38.0	35.0	40.0	28.0	41.0
39	35.49481604619625	38.0	35.0	40.0	27.0	41.0
40	35.368511308456185	38.0	35.0	40.0	27.0	41.0
41	35.37148315032737	38.0	35.0	40.0	27.0	41.0
42	35.13820668737332	38.0	34.0	40.0	26.0	41.0
43	35.166178164690784	38.0	34.0	40.0	26.0	41.0
44	34.948886652959445	37.0	34.0	40.0	26.0	41.0
45	34.72934949035391	37.0	34.0	40.0	24.0	41.0
46	34.79760852764046	37.0	34.0	40.0	25.0	41.0
47	34.66379106697581	37.0	34.0	40.0	24.0	41.0
48	34.6507458768975	37.0	34.0	40.0	24.0	41.0
49	34.579777476413376	37.0	34.0	40.0	24.0	41.0
50	34.11018854718054	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	5.0
16	18.0
17	48.0
18	119.0
19	243.0
20	475.0
21	790.0
22	1251.0
23	1813.0
24	2690.0
25	3749.0
26	4886.0
27	5432.0
28	6041.0
29	6956.0
30	8507.0
31	10833.0
32	13607.0
33	18242.0
34	28445.0
35	46659.0
36	33257.0
37	47048.0
38	53843.0
39	47926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.06847776951456	25.494845210493317	28.51830788748414	13.918369132507985
2	31.03343686658792	26.28986394855418	29.6982953468364	12.978403838021494
3	28.226081630867494	25.852107849570555	32.41261647491142	13.509194044650538
4	25.12358370882366	28.522682532044268	31.82728903276609	14.526444726365984
5	22.96017615235429	32.27846070840077	30.34311795499949	14.418245184245446
6	19.61473963573793	41.949633258964376	27.43718739519081	10.998439710106886
7	87.35902707904982	3.7849424734240342	7.134170348659172	1.721860098866967
8	54.22430260874638	40.06999431296207	4.0144654913454945	1.6912375869460607
9	83.70094929786956	5.150998148067136	8.621841142073873	2.5262114119894425
10	46.82707030053808	29.5011446986599	12.895285591379032	10.776499409422986
11	39.88538431252461	21.37713810752876	24.127331320996838	14.610146258949793
12	38.03024337605903	21.299561077329134	25.39976960205317	15.27042594455867
13	20.885719701940882	38.20172944281611	26.009303410764538	14.90324744447847
14	15.971244003091414	40.889802703530336	26.97901628825991	16.159937005118334
15	14.018402671449612	26.147250535893956	44.447555302798314	15.386791489858116
16	16.28242705280196	21.943508756580194	43.48046721203902	18.293596978578826
17	17.25884771862286	22.313895329337825	28.201000335389416	32.2262566166499
18	20.72269128133339	26.31698674482698	32.91745045715035	20.04287151668927
19	26.853609810869532	28.895693891537977	24.62983215947038	19.62086413812211
20	28.855738804555465	27.08459104364437	23.896058445251324	20.163611706548842
21	21.334849876780844	29.958732519649445	28.413608061011708	20.292809542558
22	21.639616781136535	26.66083380725316	25.88214707555011	25.817402336060198
23	18.682357058488996	33.26129751957653	23.996966913104977	24.05937850882949
24	20.150487772868455	27.988975895708474	34.77784096708809	17.08269536433498
25	18.702772066436268	28.306575090773872	31.8660775478659	21.124575294923954
26	17.924960263645247	35.04644414308004	26.07288157837176	20.955714014902956
27	18.256266678332388	33.85654082272482	27.945229450107178	19.941963048835614
28	16.230514604021756	30.759292474153142	35.339836971579395	17.670355950245707
29	16.636481619201774	26.68241538708313	34.86795864502676	21.813144348688336
30	19.644778861717484	31.09818160607784	30.607346486431307	18.649693045773365
31	26.944310774749553	30.000145821485336	23.303439928839115	19.752103474925995
32	28.083176575236596	30.565641541624743	23.106580923633288	18.244600959505377
33	24.299983959636613	31.324788194292548	23.78698397421876	20.58824387185208
34	18.751476442539044	30.361199819181355	26.903189115884334	23.984134622395263
35	18.899631071642094	29.467022471090893	29.109759832013648	22.523586625253365
36	27.674293130349824	27.557635942079706	24.831940738148358	19.936130189422112
37	19.719731105181037	32.67334529069513	27.92423115621856	19.682692447905275
38	18.831969902445426	34.29254706388439	24.681452965279902	22.194030068390276
39	19.874301879638946	32.66197121483879	25.378479665193872	22.08524724032839
40	23.11270542601747	27.951062309520687	25.321025999970836	23.61520626449101
41	18.33967656794552	26.70866325444391	26.769033349373693	28.182626828236874
42	22.049083511964653	29.651049185587002	22.539626988640507	25.760240313807834
43	22.138909546932645	29.350656925791448	25.066713329541972	23.443720197733935
44	20.323723697449584	32.5934351167301	25.92064394767925	21.16219723814107
45	18.288639048077343	35.056068361112324	23.20894760634032	23.44634498447001
46	21.814602563541712	32.92794960409467	24.931682634119312	20.32576519824431
47	21.557665106376774	29.480438047741952	25.898479081907926	23.063417763973344
48	22.77264972221007	25.954474532277587	28.27711915073567	22.995756594776676
49	20.34180556163145	25.576796885253074	29.547515930997275	24.5338816221182
50	19.048660629657174	31.82962217653149	27.188415941204774	21.93330125260656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	346.0
1	397.5
2	449.0
3	1941.0
4	3433.0
5	2366.5
6	1300.0
7	1474.5
8	1649.0
9	1891.5
10	2134.0
11	2353.0
12	2572.0
13	2560.0
14	2548.0
15	2474.0
16	2400.0
17	2312.0
18	2224.0
19	2055.0
20	1886.0
21	1897.5
22	1909.0
23	1876.5
24	1844.0
25	1992.0
26	2140.0
27	2539.5
28	2939.0
29	3505.5
30	4072.0
31	4702.5
32	5333.0
33	6520.0
34	7707.0
35	9356.0
36	11005.0
37	12268.0
38	13531.0
39	16665.5
40	19800.0
41	23977.5
42	28155.0
43	30605.0
44	33055.0
45	34038.5
46	35022.0
47	34537.5
48	34053.0
49	32485.0
50	30917.0
51	27205.5
52	23494.0
53	20398.0
54	17302.0
55	15382.5
56	13463.0
57	12037.5
58	10612.0
59	9588.5
60	8565.0
61	7527.0
62	6489.0
63	5428.5
64	4368.0
65	3320.0
66	2272.0
67	1842.5
68	1413.0
69	1211.0
70	1009.0
71	841.5
72	674.0
73	519.5
74	365.0
75	299.0
76	233.0
77	168.5
78	104.0
79	83.0
80	62.0
81	44.0
82	26.0
83	17.5
84	9.0
85	5.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	342885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.70086820917664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21765766870892	30.94940761236619
2	8.05269599580018	6.716088288988555
3	3.72539605311783	4.660567495141603
4	2.4516675770522256	4.089466660934651
5	1.7997579423284262	3.7525734380728313
6	1.4575197419029926	3.6467903201621903
7	1.1671626175973886	3.4070186146574435
8	1.0314134775822577	3.440866999826104
9	0.8456925744658802	3.173950313395288
>10	5.2138154026589625	33.862580061642696
>50	0.025984435944206026	0.7251156978558488
>100	0.009129666683099415	0.6372583838991628
>500	0.0014045641050922176	0.4688651988155146
>1k	7.022820525461088E-4	0.46945091424189245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1603	0.46750368199250475	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.2872683261151698	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	616	0.17965206993598437	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	359	0.10469982647243245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05366230660425507	0.0
2	0.0	0.0	0.0	0.16244513466614172	0.0
3	0.0	0.0	0.0	0.2653951033145224	0.0
4	0.0	0.0	0.0	0.4190909488604051	0.0
5	0.0	0.0	0.0	0.7168584219198857	0.0
6	0.0	0.0	0.0	1.254939702815813	0.0
7	0.0	0.0	0.0	1.550374032109891	0.0
8	0.0	0.0	0.0	2.2056957872172887	0.0
9	0.0	0.0	0.0	2.7268617758140485	0.0
10	0.0	0.0	0.0	3.4323461218775972	0.0
11	0.0	0.0	0.0	4.140746897647899	0.0
12	0.0	0.0	0.0	4.742697989121718	0.0
13	0.0	0.0	0.0	5.070796331131429	0.0
14	0.0	0.0	0.0	5.231199965002843	0.0
15	0.0	0.0	0.0	5.375271592516441	0.0
16	0.0	0.0	0.0	5.638041909094886	0.0
17	0.0	0.0	0.0	5.941058955626522	0.0
18	0.0	0.0	0.0	6.360733190428277	0.0
19	0.0	0.0	0.0	6.576548988727999	0.0
20	0.0	0.0	0.0	6.803155576942706	0.0
21	0.0	0.0	0.0	7.071758752934658	0.0
22	0.0	0.0	0.0	7.346486431310789	0.0
23	0.0	0.0	0.0	7.6565029091386325	0.0
24	0.0	0.0	0.0	7.909357364714117	0.0
25	0.0	0.0	0.0	8.114674016069527	0.0
26	0.0	0.0	0.0	8.3074500196859	0.0
27	0.0	0.0	0.0	8.494976449830117	0.0
28	0.0	0.0	0.0	8.712833748924567	0.0
29	0.0	0.0	0.0	8.939440337139274	0.0
30	0.0	0.0	0.0	9.20775187016055	0.0
31	0.0	0.0	0.0	9.475480117240474	0.0
32	0.0	0.0	0.0	9.693337416334924	0.0
33	0.0	0.0	0.0	9.927235078816512	0.0
34	0.0	0.0	0.0	10.14450909196961	0.0
35	0.0	0.0	0.0	10.413987196873588	0.0
36	0.0	0.0	0.0	10.674132726715955	0.0
37	0.0	0.0	0.0	10.91765460722983	0.0
38	0.0	0.0	0.0	11.159426629919652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTAGT	20	7.8487437E-4	44.000004	7
ATATGCG	20	7.8487437E-4	44.000004	1
CGTTAGA	20	7.8487437E-4	44.000004	2
CGCTCCC	20	7.8487437E-4	44.000004	19
TATCTAG	20	7.8487437E-4	44.000004	1
AGCGATA	65	0.0	44.000004	8
GCGATAC	40	8.283678E-9	44.000004	9
ATGGTCG	20	7.8487437E-4	44.000004	44
TATACGA	20	7.8487437E-4	44.000004	2
ACGAGCC	20	7.8487437E-4	44.000004	5
CCGTTGG	20	7.8487437E-4	44.000004	1
ACCGCTC	35	1.442113E-7	44.0	18
TGAACGC	25	4.4362845E-5	44.0	29
AAGCGAC	30	2.5225017E-6	44.0	15
CGCGACC	90	0.0	44.0	10
TATGCGA	25	4.4362845E-5	44.0	2
GTAGACG	25	4.4362845E-5	44.0	1
CTACGGG	30	2.5225017E-6	44.0	3
TCGCTAT	30	2.5225017E-6	44.0	23
AGACCGA	100	0.0	44.0	8
>>END_MODULE