FastQCFastQC Report
Thu 26 May 2016
SRR1547155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547155_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1654165
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.1726550857985751No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20860.1261059205097436No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.10936031169804705No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.857486E-444.042
ACGTTAG408.3164196E-944.01
CGTTAGA551.8189894E-1244.02
CTAACGT254.4436903E-544.027
ACGCGCT254.4436903E-544.016
TACGAGT501.3478711E-939.6000024
TTCGACA452.3519533E-839.1111143
CGTTTTT34950.039.027181
CACAACG3700.038.6486512
GAGCGAT12450.038.5220877
TATCACG404.1259955E-738.51
AGCGTAT404.1259955E-738.521
CGACGGT1600.038.528
ACGAGAT1450.037.9310385
GCGCGAC2650.037.358499
TTTCGCA900.036.66666813
ACGGGCC601.9826984E-1036.6666685
GTCCGAT301.3013421E-436.66666814
ACGTCGA301.3013421E-436.6666682
ATACGCG301.3013421E-436.6666681
Produced by FastQC (version 0.11.5)