##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547155_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1654165
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558122678209248	31.0	31.0	33.0	30.0	34.0
2	31.956023129494337	31.0	31.0	34.0	30.0	34.0
3	32.1203241514601	33.0	31.0	34.0	30.0	34.0
4	35.79714296941357	37.0	35.0	37.0	35.0	37.0
5	35.70224917103191	37.0	35.0	37.0	33.0	37.0
6	35.75262624949748	37.0	35.0	37.0	35.0	37.0
7	36.03512708828926	37.0	35.0	37.0	35.0	37.0
8	22.025496851885997	35.0	0.0	37.0	0.0	37.0
9	29.96199230427436	37.0	18.0	39.0	17.0	39.0
10	34.39249168009237	35.0	32.0	39.0	27.0	39.0
11	36.02806854213455	37.0	35.0	39.0	32.0	39.0
12	36.47000571285211	37.0	35.0	39.0	33.0	39.0
13	36.60780393733394	39.0	35.0	39.0	33.0	39.0
14	37.70215969990902	39.0	36.0	41.0	33.0	41.0
15	37.93389534901294	40.0	36.0	41.0	33.0	41.0
16	37.989979234236	40.0	36.0	41.0	33.0	41.0
17	37.94065706867211	40.0	36.0	41.0	33.0	41.0
18	37.904164336689504	40.0	36.0	41.0	33.0	41.0
19	37.934189757370035	40.0	36.0	41.0	33.0	41.0
20	37.87295825990757	40.0	36.0	41.0	33.0	41.0
21	37.77081488243313	40.0	36.0	41.0	33.0	41.0
22	37.7309476382344	40.0	36.0	41.0	33.0	41.0
23	37.61861362076939	39.0	35.0	41.0	33.0	41.0
24	37.55059440865936	39.0	35.0	41.0	33.0	41.0
25	37.40795990726439	39.0	35.0	41.0	32.0	41.0
26	37.35717718607273	39.0	35.0	41.0	32.0	41.0
27	37.2758624441939	39.0	35.0	41.0	32.0	41.0
28	37.3107350234106	39.0	35.0	41.0	32.0	41.0
29	37.223142794098536	39.0	35.0	41.0	32.0	41.0
30	37.136432580788494	39.0	35.0	41.0	32.0	41.0
31	37.10254055671593	39.0	35.0	41.0	32.0	41.0
32	36.95100911940465	39.0	35.0	41.0	31.0	41.0
33	36.885984167238455	39.0	35.0	41.0	31.0	41.0
34	36.840511073562794	39.0	35.0	41.0	31.0	41.0
35	36.7182505977336	39.0	35.0	41.0	31.0	41.0
36	36.62413423086572	39.0	35.0	41.0	31.0	41.0
37	36.60228393177222	39.0	35.0	41.0	31.0	41.0
38	36.50308705600711	39.0	35.0	41.0	30.0	41.0
39	36.42076213678805	39.0	35.0	41.0	30.0	41.0
40	36.345602766350396	39.0	35.0	41.0	30.0	41.0
41	36.330280836555	39.0	35.0	41.0	30.0	41.0
42	36.10646700903477	38.0	35.0	40.0	30.0	41.0
43	36.12500989925431	38.0	35.0	40.0	30.0	41.0
44	35.93296738838024	38.0	35.0	40.0	30.0	41.0
45	35.72142984526936	38.0	35.0	40.0	29.0	41.0
46	35.77287574093273	38.0	35.0	40.0	29.0	41.0
47	35.643944830171115	38.0	35.0	40.0	29.0	41.0
48	35.64550513401021	38.0	35.0	40.0	29.0	41.0
49	35.566103139650515	38.0	34.0	40.0	29.0	41.0
50	35.116849286498024	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	2.0
13	4.0
14	14.0
15	38.0
16	60.0
17	154.0
18	383.0
19	752.0
20	1364.0
21	2388.0
22	3840.0
23	5827.0
24	8399.0
25	11956.0
26	15955.0
27	19511.0
28	22898.0
29	27957.0
30	35394.0
31	45848.0
32	59674.0
33	80293.0
34	126027.0
35	190767.0
36	166476.0
37	251537.0
38	292602.0
39	284038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.21325563048426	23.21950954106755	28.080753733756907	12.486481094691278
2	31.286721699467705	25.573385968147072	28.815867824551965	14.324024507833258
3	30.730791668303947	25.330544413646766	29.618327071362287	14.320336846686999
4	26.736631472676546	28.010506811593768	29.1460646307956	16.10679708493409
5	23.492215105506403	32.848778688945785	28.194345787753942	15.464660417793871
6	21.329311163033918	41.40838429056352	26.2250138287293	11.037290717673267
7	89.09733914089585	3.9681652072193527	5.191803719701481	1.742691932183307
8	54.99916876490556	40.14907823584709	3.1761644092336616	1.6755885900136929
9	84.94702765443593	4.711682329150961	7.049780402801413	3.291509613611701
10	44.50886096610677	25.699310528272573	15.205012801020453	14.586815704600205
11	36.63649031384414	25.468015584902354	20.996273044103823	16.89922105714968
12	32.96297527755696	23.89543969313823	24.904226603754765	18.237358425550052
13	23.433696154857586	32.492828708139754	26.616087270616895	17.45738786638576
14	20.069944654856076	34.479631717513065	27.509347616471153	17.94107601115971
15	18.834275903552548	25.295481406026603	37.68940825129295	18.1808344391279
16	21.60824343399842	23.15113667620824	35.88710920615537	19.353510683637968
17	21.647477730456153	22.85624469143042	27.554143631378974	27.942133946734454
18	24.505475572267578	24.262815378151515	30.364927319826013	20.86678172975489
19	27.921035688700947	26.418464905254314	25.487360692554855	20.173138713489887
20	30.328836603361818	25.36210112050491	24.62517342586743	19.683888850265845
21	25.640670670700928	27.081337109659557	26.774717153367412	20.503275066272106
22	25.457013054924992	24.05539955203985	26.204580558771344	24.283006834263812
23	22.458400461864443	29.217399715264197	25.681960384846736	22.64223943802462
24	22.284899027606073	25.992751629976453	31.572545665033413	20.149803677384057
25	22.302672345261808	26.738445076518968	29.594266593719492	21.364615984499732
26	20.88473640779487	31.03964840266842	25.983018622688785	22.092596566847927
27	21.252232999730982	29.415203441011023	27.877448743021404	21.45511481623659
28	19.76169245510575	27.570163798653702	32.880395849265334	19.787747896975212
29	20.49898287051171	26.37693337726285	31.86616812712154	21.257915625103905
30	21.781019426719826	27.51321663800165	30.10455426151563	20.601209673762895
31	27.276480883104163	26.189467193417826	25.440146539190465	21.093905384287538
32	27.517206566454977	26.850163073212162	26.237769509087666	19.39486085124519
33	25.219551858490536	26.778223454129424	26.01977432722854	21.982450360151496
34	21.63127620279718	27.18743293444124	28.834608397590326	22.34668246517125
35	21.315527773831512	27.2581634842957	29.696614303893504	21.729694437979283
36	27.027170808232555	27.423624608186003	26.014273062239862	19.534931521341584
37	22.716536742102512	28.966034222704508	27.341951981815598	20.975477053377382
38	22.58662225352368	30.73768336290515	24.824428034688196	21.851266348882973
39	22.314218956391898	28.370748988160187	26.479341540898275	22.835690514549636
40	24.319278911112253	26.18608179957864	26.02098339645682	23.473655892852285
41	20.41646389568151	26.14376437658879	27.117851000353653	26.321920727376046
42	23.573101836878426	27.021971810550944	25.235874293072335	24.1690520594983
43	23.138743716618354	26.425961134469656	26.446152590581956	23.989142558330034
44	22.56582626279724	28.2294087953741	27.565569335586233	21.639195606242424
45	21.416545507854416	30.4129878216502	25.097375412972706	23.073091257522677
46	23.232688395655813	28.718175030906835	26.659432402450783	21.38970417098657
47	22.81580132574441	27.34981093179943	27.151523578361285	22.682864164094877
48	23.13154975471008	25.6164892861353	28.721076797054707	22.530884162099913
49	23.126411210489884	24.650322065815683	29.225258665248028	22.998008058446405
50	20.76334585727542	28.973409544997025	28.104149223324153	22.1590953744034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	811.0
1	1442.0
2	2073.0
3	5438.0
4	8803.0
5	6537.5
6	4272.0
7	4459.0
8	4646.0
9	4936.5
10	5227.0
11	5331.0
12	5435.0
13	5462.0
14	5489.0
15	5329.5
16	5170.0
17	5202.5
18	5235.0
19	5538.0
20	5841.0
21	6412.5
22	6984.0
23	7782.0
24	8580.0
25	9882.5
26	11185.0
27	13202.5
28	15220.0
29	18673.5
30	22127.0
31	25829.0
32	29531.0
33	34732.0
34	39933.0
35	44844.0
36	49755.0
37	56725.0
38	63695.0
39	74716.5
40	85738.0
41	100470.5
42	115203.0
43	127655.5
44	140108.0
45	143937.0
46	147766.0
47	145145.5
48	142525.0
49	136684.0
50	130843.0
51	123659.5
52	116476.0
53	107806.0
54	99136.0
55	91824.5
56	84513.0
57	78597.0
58	72681.0
59	67859.0
60	63037.0
61	57723.0
62	52409.0
63	45746.0
64	39083.0
65	32172.0
66	25261.0
67	20299.0
68	15337.0
69	12409.0
70	9481.0
71	7783.0
72	6085.0
73	5010.5
74	3936.0
75	3162.0
76	2388.0
77	1796.0
78	1204.0
79	856.0
80	508.0
81	365.0
82	222.0
83	168.0
84	114.0
85	83.5
86	53.0
87	38.0
88	23.0
89	18.0
90	13.0
91	10.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1654165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.227838666048775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24103517028142	16.72445590831517
2	6.022253310882738	2.677233500007592
3	2.322505727779946	1.5487284785420052
4	1.3987966939780483	1.2436890896138582
5	1.0515495116273512	1.1686836446907574
6	0.8740473887426128	1.165691060607121
7	0.7192768829219229	1.1191579356864871
8	0.6348256672699105	1.1288642010513865
9	0.5651010460547108	1.1304877393547563
>10	10.022369793633452	54.36348275810635
>50	1.088591953577934	15.148941090784984
>100	0.05744789094588953	1.9950851025934542
>500	0.0013743514401041023	0.17302875482726987
>1k	8.246108640624614E-4	0.4124707358188202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2856	0.1726550857985751	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2086	0.1261059205097436	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1809	0.10936031169804705	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029440835708650588	0.0
2	0.0	0.0	0.0	0.09158699404231138	0.0
3	0.0	0.0	0.0	0.13547620702892396	0.0
4	0.0	0.0	0.0	0.2371589291273845	0.0
5	0.0	0.0	0.0	0.4003832749453652	0.0
6	0.0	0.0	0.0	0.6681921090096816	0.0
7	0.0	0.0	0.0	0.7999202014309335	0.0
8	0.0	0.0	0.0	1.186217819866821	0.0
9	0.0	0.0	0.0	1.4763944346543423	0.0
10	0.0	0.0	0.0	1.9264100014206564	0.0
11	0.0	0.0	0.0	2.2882239679838468	0.0
12	0.0	0.0	0.0	2.616546716923644	0.0
13	0.0	0.0	0.0	2.760305048166295	0.0
14	0.0	0.0	0.0	2.818823998815112	0.0
15	0.0	0.0	0.0	2.885383259832	0.0
16	0.0	0.0	0.0	3.034521949140503	0.0
17	0.0	0.0	0.0	3.1965372257302023	0.0
18	0.0	0.0	0.0	3.427408994870524	0.0
19	0.0	0.0	0.0	3.538764270795235	0.0
20	0.0	0.0	0.0	3.6636611220766975	0.0
21	0.0	0.0	0.0	3.823137353287006	0.0
22	0.0	0.0	0.0	3.981464968730447	0.0
23	0.0	0.0	0.0	4.15363642683771	0.0
24	0.0	0.0	0.0	4.306160509985401	0.0
25	0.0	0.0	0.0	4.425012015125456	0.0
26	0.0	0.0	0.0	4.5372136395099645	0.0
27	0.0	0.0	0.0	4.648448008511847	0.0
28	0.0	0.0	0.0	4.773707580561794	0.0
29	0.0	0.0	0.0	4.906886556056984	0.0
30	0.0	0.0	0.0	5.0844988256915125	0.0
31	0.0	0.0	0.0	5.231521643850523	0.0
32	0.0	0.0	0.0	5.38265529738569	0.0
33	0.0	0.0	0.0	5.5349375666877245	0.0
34	0.0	0.0	0.0	5.6815372106168365	0.0
35	0.0	0.0	0.0	5.860419002941061	0.0
36	0.0	0.0	0.0	6.009618145710978	0.0
37	0.0	0.0	0.0	6.160691345784731	0.0
38	0.0	0.0	0.0	6.330142398128361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.857486E-4	44.0	42
ACGTTAG	40	8.3164196E-9	44.0	1
CGTTAGA	55	1.8189894E-12	44.0	2
CTAACGT	25	4.4436903E-5	44.0	27
ACGCGCT	25	4.4436903E-5	44.0	16
TACGAGT	50	1.3478711E-9	39.600002	4
TTCGACA	45	2.3519533E-8	39.11111	43
CGTTTTT	3495	0.0	39.02718	1
CACAACG	370	0.0	38.64865	12
GAGCGAT	1245	0.0	38.522087	7
TATCACG	40	4.1259955E-7	38.5	1
AGCGTAT	40	4.1259955E-7	38.5	21
CGACGGT	160	0.0	38.5	28
ACGAGAT	145	0.0	37.931038	5
GCGCGAC	265	0.0	37.35849	9
TTTCGCA	90	0.0	36.666668	13
ACGGGCC	60	1.9826984E-10	36.666668	5
GTCCGAT	30	1.3013421E-4	36.666668	14
ACGTCGA	30	1.3013421E-4	36.666668	2
ATACGCG	30	1.3013421E-4	36.666668	1
>>END_MODULE