FastQCFastQC Report
Thu 26 May 2016
SRR1547154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547154_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1704511
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35270.2069215159069082No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.1981213380259793No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.12396517241601843No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT254.4437482E-544.031
ATTACGA351.4466423E-744.02
TTATCGC254.4437482E-544.012
TCGATAT600.044.016
CATTACG302.5284407E-644.016
TACGAAC254.4437482E-544.043
AATACGA502.7284841E-1144.02
TTCGATA650.040.61538315
TTAGCGA1250.040.482
ATCGTAG557.8216544E-1140.01
ATCGTTA501.3478711E-939.638
ACGTATA501.3478711E-939.623
CGTTTTT41550.039.552351
GTCGATG452.3521352E-839.111111
CTCACGA1200.038.524
CGGTCTA1200.038.531
AGGGGAA111500.038.100458
TATTGCG1050.037.7142871
ATCGGCA357.2922776E-637.71428724
CGCCCGT357.2922776E-637.71428715
Produced by FastQC (version 0.11.5)