##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547154_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1704511
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51803420453139	31.0	31.0	33.0	30.0	34.0
2	31.914066849671254	31.0	31.0	34.0	30.0	34.0
3	32.075590007926024	33.0	31.0	34.0	30.0	34.0
4	35.76509626514584	37.0	35.0	37.0	35.0	37.0
5	35.669446545079495	37.0	35.0	37.0	33.0	37.0
6	35.72321680528903	37.0	35.0	37.0	33.0	37.0
7	35.979754310767134	37.0	35.0	37.0	35.0	37.0
8	21.820139617755473	35.0	0.0	37.0	0.0	37.0
9	29.83141440565652	37.0	18.0	39.0	17.0	39.0
10	34.382567199624994	35.0	32.0	39.0	27.0	39.0
11	36.020652844129486	37.0	35.0	39.0	32.0	39.0
12	36.26862836320798	37.0	35.0	39.0	32.0	39.0
13	36.296468605952086	38.0	35.0	39.0	32.0	39.0
14	37.2920380097283	39.0	35.0	41.0	32.0	41.0
15	37.60324984702358	39.0	35.0	41.0	33.0	41.0
16	37.70401188376021	40.0	35.0	41.0	33.0	41.0
17	37.66731220860411	39.0	35.0	41.0	33.0	41.0
18	37.65580685604258	39.0	36.0	41.0	33.0	41.0
19	37.66329756745483	39.0	36.0	41.0	33.0	41.0
20	37.567431363012616	39.0	35.0	41.0	33.0	41.0
21	37.452095058348114	39.0	35.0	41.0	33.0	41.0
22	37.43119522255943	39.0	35.0	41.0	33.0	41.0
23	37.33681507482205	39.0	35.0	41.0	32.0	41.0
24	37.220105355729594	39.0	35.0	41.0	32.0	41.0
25	37.066801563615606	39.0	35.0	41.0	32.0	41.0
26	36.993646858248496	39.0	35.0	41.0	32.0	41.0
27	36.88577017103439	39.0	35.0	41.0	32.0	41.0
28	36.90663715282565	39.0	35.0	41.0	32.0	41.0
29	36.80838786021328	38.0	35.0	41.0	31.0	41.0
30	36.720899425113714	38.0	35.0	40.0	31.0	41.0
31	36.62063782515924	38.0	35.0	40.0	31.0	41.0
32	36.4371247824156	38.0	35.0	40.0	31.0	41.0
33	36.37997936064948	38.0	35.0	41.0	30.0	41.0
34	36.321107343983115	38.0	35.0	41.0	30.0	41.0
35	36.185217930538435	38.0	35.0	41.0	30.0	41.0
36	36.03480001009087	38.0	35.0	40.0	30.0	41.0
37	35.9686091788202	38.0	35.0	40.0	30.0	41.0
38	35.86605190579586	38.0	35.0	40.0	30.0	41.0
39	35.76418926014558	38.0	35.0	40.0	29.0	41.0
40	35.61999130542426	38.0	35.0	40.0	29.0	41.0
41	35.58735261902094	38.0	35.0	40.0	29.0	41.0
42	35.34572554826575	38.0	34.0	40.0	27.0	41.0
43	35.35057503295667	38.0	34.0	40.0	28.0	41.0
44	35.1450545053684	37.0	34.0	40.0	27.0	41.0
45	34.94676244389153	37.0	34.0	40.0	26.0	41.0
46	35.0119899490235	37.0	34.0	40.0	27.0	41.0
47	34.85473429036246	37.0	34.0	40.0	26.0	41.0
48	34.85054188561998	36.0	34.0	40.0	27.0	41.0
49	34.76585425380065	36.0	34.0	40.0	26.0	41.0
50	34.282669340356264	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	5.0
12	4.0
13	8.0
14	25.0
15	40.0
16	107.0
17	240.0
18	515.0
19	1126.0
20	2069.0
21	3520.0
22	5247.0
23	7826.0
24	11138.0
25	15351.0
26	19797.0
27	24410.0
28	28429.0
29	33378.0
30	41229.0
31	52237.0
32	67384.0
33	90140.0
34	145753.0
35	228017.0
36	170123.0
37	247023.0
38	277571.0
39	231795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58586597563759	23.8757626087482	27.601816591386036	12.93655482422818
2	30.680060146282422	25.825764691456964	29.334336944730776	14.159838217529838
3	30.22368292137745	25.777129041701695	29.650145994951043	14.34904204196981
4	25.73776291264768	29.06904091554704	29.159624079868067	16.033572091937216
5	23.74229324422078	32.649129281066536	28.20726882959394	15.401308645118746
6	21.02368362539168	41.37321495725167	26.14174974523485	11.461351672121799
7	87.33472532591459	4.880989327730944	5.807472055035139	1.9768132913193286
8	53.75653193203212	40.95121709393486	3.3045254621413416	1.9877255118916803
9	83.84838818875325	5.164003048381618	7.640725111190247	3.346883651674879
10	51.349624613745526	23.476175865101485	12.758908566738494	12.415290954414491
11	44.238024864609265	22.54265299549255	19.06476402909691	14.154558110801279
12	38.85589474048569	21.762898567389712	22.958725405702868	16.422481286421736
13	25.14158019514101	34.515647009611556	24.1555495975092	16.187223197738238
14	18.785211711746065	36.79448240580436	27.39448440051135	17.025821481938223
15	17.630980380883432	23.40882517038611	41.354675915849185	17.605518532881277
16	20.944071349495545	19.91832261569447	40.086452947502245	19.05115308730774
17	21.09396771273403	20.637179812861284	26.54374187083568	31.725110603569
18	25.21133627181051	22.369700166206027	30.919542320348768	21.499421241634696
19	29.275434420781092	25.84964250744055	23.91712344478856	20.957799626989793
20	31.95714196036283	24.480041489905314	22.97820313274599	20.58461341698587
21	25.370267484340083	28.043292181745965	26.129840171169327	20.456600162744625
22	24.926973190551426	23.2767051664671	26.34022309037607	25.45609855260541
23	22.68099179178075	29.444691175357622	23.342589164868986	24.53172786799264
24	23.489904142595737	25.13688676693785	32.45869343172323	18.914515658743184
25	21.762487892421934	24.581947549766472	30.341194630014122	23.314369927797472
26	20.05994681172489	30.49607776071847	26.02658475069976	23.41739067685688
27	21.389595021680705	29.143373084714618	27.342211343898633	22.124820549706044
28	18.126782402694968	29.145015784585727	33.0271262549787	19.70107555774061
29	20.47701657542838	25.81144973543732	31.993164021822096	21.71836966731221
30	22.231361369917824	27.425930369472535	30.155276205316362	20.18743205529328
31	29.32624078108032	25.462962691352537	24.22184427087886	20.988952256688282
32	29.315387228360507	26.814963353125908	24.61351085443274	19.256138564080842
33	27.454208274396585	26.349961953897626	24.790277094134332	21.405552677571457
34	21.23553324091191	27.958165127711116	28.65549122299592	22.150810408381055
35	21.00661128030268	28.159982540447082	28.4380094936319	22.395396685618337
36	29.161032108329017	25.980471818603696	24.987518414372214	19.870977658695075
37	23.17034034981294	29.590304785360726	26.460609523787177	20.77874534103916
38	23.568343061441084	30.817284253372375	22.674068985181087	22.940303700005458
39	23.1548520367425	28.269984763958693	24.83574467985246	23.73941851944634
40	25.92919611548415	24.2989338291158	24.47693209372072	25.29493796167933
41	21.08804225962754	23.70609517920389	26.13582429212836	29.07003826904021
42	24.703096665260592	25.856271974777517	23.607943861905262	25.83268749805663
43	24.024896289903673	25.390155886350986	25.2295819739503	25.355365849795042
44	22.35045711057306	27.760513132505455	26.373663766323595	23.51536599059789
45	21.277832762592908	30.551108206400546	23.67752393501714	24.493535095989408
46	24.285792230146946	28.514512373343443	25.818372542036983	21.381322854472632
47	23.262214206889837	26.415611280889355	26.613908622472955	23.70826588974785
48	24.791039776217342	24.055579576781845	28.07984225387809	23.07353839312272
49	23.957780266598455	22.311501656486815	29.633718996239978	24.09699908067475
50	21.432657225444718	28.27239014591282	27.33687256931753	22.95808005932493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1330.0
1	2012.5
2	2695.0
3	6533.0
4	10371.0
5	7751.0
6	5131.0
7	5527.5
8	5924.0
9	6448.0
10	6972.0
11	7368.5
12	7765.0
13	7675.5
14	7586.0
15	7322.5
16	7059.0
17	6808.0
18	6557.0
19	6472.0
20	6387.0
21	6632.5
22	6878.0
23	7041.0
24	7204.0
25	7761.0
26	8318.0
27	9577.0
28	10836.0
29	12999.5
30	15163.0
31	17430.0
32	19697.0
33	22764.5
34	25832.0
35	30008.5
36	34185.0
37	40774.0
38	47363.0
39	63396.0
40	79429.0
41	100647.5
42	121866.0
43	135230.0
44	148594.0
45	153856.0
46	159118.0
47	153783.0
48	148448.0
49	139976.5
50	131505.0
51	124025.5
52	116546.0
53	110455.0
54	104364.0
55	97736.0
56	91108.0
57	85771.5
58	80435.0
59	76988.0
60	73541.0
61	69142.0
62	64743.0
63	55440.5
64	46138.0
65	38962.5
66	31787.0
67	27007.5
68	22228.0
69	18737.0
70	15246.0
71	12898.0
72	10550.0
73	8599.5
74	6649.0
75	5469.0
76	4289.0
77	3475.0
78	2661.0
79	1855.5
80	1050.0
81	811.5
82	573.0
83	420.0
84	267.0
85	166.0
86	65.0
87	45.5
88	26.0
89	19.5
90	13.0
91	10.5
92	8.0
93	7.5
94	7.0
95	5.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1704511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.575706951001674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36761289722818	17.692042632173628
2	6.497435753718397	2.933684110178145
3	2.297535842265462	1.5560548765322364
4	1.3355070552127475	1.206000636379128
5	0.8599305111801909	0.970676960931453
6	0.6494796354903131	0.8797477152883617
7	0.5365700025933551	0.8479412996072053
8	0.4498569567944736	0.8124671061169163
9	0.35804437283001106	0.7274794352818964
>10	6.686165443968816	39.35706334908763
>50	1.7928000392757812	26.90440093166545
>100	0.16617379320930287	5.239878551610162
>500	0.002100142714936493	0.3381675627734298
>1k	7.875535181011849E-4	0.5343948323743457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3527	0.2069215159069082	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3377	0.1981213380259793	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2113	0.12396517241601843	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047403624851937005	0.0
2	0.0	0.0	0.0	0.12795458638870622	0.0
3	0.0	0.0	0.0	0.1821050142826887	0.0
4	0.0	0.0	0.0	0.3136970075288455	0.0
5	0.0	0.0	0.0	0.4982660716181943	0.0
6	0.0	0.0	0.0	0.8652921571054689	0.0
7	0.0	0.0	0.0	1.0253380588332959	0.0
8	0.0	0.0	0.0	1.4926275043106205	0.0
9	0.0	0.0	0.0	1.824276874716561	0.0
10	0.0	0.0	0.0	2.357332982890694	0.0
11	0.0	0.0	0.0	2.88510898433627	0.0
12	0.0	0.0	0.0	3.3613159433996027	0.0
13	0.0	0.0	0.0	3.602851492304831	0.0
14	0.0	0.0	0.0	3.704112205788053	0.0
15	0.0	0.0	0.0	3.8098316760642787	0.0
16	0.0	0.0	0.0	4.016694524118647	0.0
17	0.0	0.0	0.0	4.237578988929963	0.0
18	0.0	0.0	0.0	4.54605455758279	0.0
19	0.0	0.0	0.0	4.693604206719699	0.0
20	0.0	0.0	0.0	4.83827913108217	0.0
21	0.0	0.0	0.0	5.027013612701825	0.0
22	0.0	0.0	0.0	5.206361237915155	0.0
23	0.0	0.0	0.0	5.4170374963845935	0.0
24	0.0	0.0	0.0	5.583478194039229	0.0
25	0.0	0.0	0.0	5.716595551451413	0.0
26	0.0	0.0	0.0	5.8537609906888255	0.0
27	0.0	0.0	0.0	5.985411651787522	0.0
28	0.0	0.0	0.0	6.118763680609864	0.0
29	0.0	0.0	0.0	6.265198640548521	0.0
30	0.0	0.0	0.0	6.4661360354963975	0.0
31	0.0	0.0	0.0	6.626416608634383	0.0
32	0.0	0.0	0.0	6.787811870970619	0.0
33	0.0	0.0	0.0	6.947095090615432	0.0
34	0.0	0.0	0.0	7.116703852307201	0.0
35	0.0	0.0	0.0	7.309369080047005	0.0
36	0.0	0.0	0.0	7.477159138310049	0.0
37	0.0	0.0	0.0	7.643306496701987	0.0
38	0.0	0.0	0.0	7.866068332794567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAT	25	4.4437482E-5	44.0	31
ATTACGA	35	1.4466423E-7	44.0	2
TTATCGC	25	4.4437482E-5	44.0	12
TCGATAT	60	0.0	44.0	16
CATTACG	30	2.5284407E-6	44.0	16
TACGAAC	25	4.4437482E-5	44.0	43
AATACGA	50	2.7284841E-11	44.0	2
TTCGATA	65	0.0	40.615383	15
TTAGCGA	125	0.0	40.48	2
ATCGTAG	55	7.8216544E-11	40.0	1
ATCGTTA	50	1.3478711E-9	39.6	38
ACGTATA	50	1.3478711E-9	39.6	23
CGTTTTT	4155	0.0	39.55235	1
GTCGATG	45	2.3521352E-8	39.11111	1
CTCACGA	120	0.0	38.5	24
CGGTCTA	120	0.0	38.5	31
AGGGGAA	11150	0.0	38.10045	8
TATTGCG	105	0.0	37.714287	1
ATCGGCA	35	7.2922776E-6	37.714287	24
CGCCCGT	35	7.2922776E-6	37.714287	15
>>END_MODULE