FastQCFastQC Report
Thu 26 May 2016
SRR1547153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547153_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3115027
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100730.32336798364829583No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47040.1510099270407608No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT207.858557E-444.00000443
TCCGTCG207.858557E-444.0000041
ACGTCGA408.3200575E-944.00000419
TTACGTA408.3200575E-944.00000440
CGTCGGA207.858557E-444.00000412
GTCGACG254.4445995E-544.032
CGTTGCA254.4445995E-544.015
GTTCGAC254.4445995E-544.015
TTAACGC302.5291174E-644.021
CGTCGGT302.5291174E-644.032
CGGTCGA254.4445995E-544.027
TCGACGA254.4445995E-544.033
CCGTTCG254.4445995E-544.026
CGCATCG650.043.99999621
TCCCGTA351.4471516E-743.99999638
TCGTTAG1300.042.307691
CTTGCGG1650.041.3333322
CGGTCTA2450.041.3061231
CCGACAA750.041.0666740
ACGTCGG700.040.857142
Produced by FastQC (version 0.11.5)