##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547153_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3115027
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.999392621636986	31.0	31.0	33.0	30.0	34.0
2	31.37502692593034	31.0	31.0	34.0	30.0	34.0
3	31.518050726366095	31.0	31.0	34.0	30.0	34.0
4	35.271076302067364	37.0	35.0	37.0	33.0	37.0
5	35.046163965834005	37.0	35.0	37.0	32.0	37.0
6	35.11784681160067	37.0	35.0	37.0	32.0	37.0
7	35.69360393986954	37.0	35.0	37.0	35.0	37.0
8	35.755884940965196	37.0	35.0	37.0	35.0	37.0
9	37.55003632392271	39.0	37.0	39.0	35.0	39.0
10	36.77841636685653	39.0	37.0	39.0	32.0	39.0
11	36.32837853411864	38.0	35.0	39.0	32.0	39.0
12	35.446024384379335	37.0	35.0	39.0	30.0	39.0
13	35.05007725454707	37.0	34.0	39.0	29.0	39.0
14	35.92472874231909	38.0	34.0	40.0	29.0	41.0
15	36.30814885392647	38.0	35.0	40.0	30.0	41.0
16	36.45788046138926	38.0	35.0	40.0	31.0	41.0
17	36.37542949065931	38.0	35.0	40.0	31.0	41.0
18	36.350707714571975	38.0	35.0	40.0	31.0	41.0
19	36.31481556981689	37.0	35.0	40.0	31.0	41.0
20	36.14514834060829	37.0	34.0	40.0	30.0	41.0
21	35.97357422584138	37.0	34.0	40.0	30.0	41.0
22	35.88257726177012	37.0	34.0	40.0	30.0	41.0
23	35.77093938511609	37.0	34.0	40.0	30.0	41.0
24	35.76043578434473	37.0	34.0	40.0	30.0	41.0
25	35.64445412511673	37.0	34.0	40.0	29.0	41.0
26	35.523635589675465	36.0	34.0	40.0	29.0	41.0
27	35.34483746047787	36.0	34.0	40.0	29.0	41.0
28	35.38170423562942	36.0	34.0	40.0	29.0	41.0
29	35.34640213391409	36.0	34.0	40.0	29.0	41.0
30	35.31134144262634	36.0	34.0	40.0	29.0	41.0
31	35.20776160206637	36.0	34.0	40.0	29.0	41.0
32	35.008196076631116	36.0	34.0	40.0	27.0	41.0
33	34.843797180570185	36.0	33.0	40.0	27.0	41.0
34	34.839928835287786	36.0	34.0	40.0	27.0	41.0
35	34.62644497142401	36.0	33.0	40.0	25.0	41.0
36	34.36662603566518	36.0	33.0	40.0	24.0	41.0
37	34.42935422389597	36.0	33.0	40.0	24.0	41.0
38	34.44473932328676	36.0	33.0	40.0	24.0	41.0
39	34.4146981711555	36.0	33.0	40.0	24.0	41.0
40	34.369985878132034	36.0	33.0	40.0	24.0	41.0
41	34.470160932794485	36.0	33.0	40.0	24.0	41.0
42	34.24596094993719	36.0	33.0	40.0	24.0	41.0
43	34.308182561499464	36.0	33.0	40.0	24.0	41.0
44	34.114721638046795	35.0	33.0	40.0	23.0	41.0
45	33.940851556021826	35.0	33.0	40.0	23.0	41.0
46	34.03309473722057	35.0	33.0	40.0	23.0	41.0
47	33.90084355609117	35.0	33.0	40.0	23.0	41.0
48	33.98250769575994	35.0	33.0	40.0	23.0	41.0
49	33.94231350161652	36.0	33.0	40.0	24.0	41.0
50	33.40482923583006	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	8.0
14	27.0
15	80.0
16	249.0
17	724.0
18	1651.0
19	3252.0
20	5774.0
21	9602.0
22	14388.0
23	21068.0
24	29458.0
25	40671.0
26	53087.0
27	63943.0
28	73740.0
29	85474.0
30	102498.0
31	124831.0
32	153463.0
33	194205.0
34	303438.0
35	416589.0
36	227791.0
37	270155.0
38	373291.0
39	545564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.155749211804583	25.404787823668944	30.19652799157118	13.242934972955291
2	29.385555887637572	26.87379595746682	30.37485710396732	13.36579105092829
3	28.33134351644464	26.20426725033202	31.897861559466417	13.56652767375692
4	25.210824817890824	28.94652277492298	31.272377414385172	14.570274992801025
5	22.756528274072746	33.12606921224118	30.113254235035527	14.004148278650556
6	20.49263136402991	41.40692841506671	27.677705522295632	10.422734698607748
7	88.75056299672522	4.364617064314371	5.502327909196293	1.382492029764108
8	90.53610129221994	3.0353508974400545	4.930711676014365	1.4978361343256414
9	86.05649325029927	5.6835783445857775	6.369222481859708	1.89070592325524
10	46.123645156205704	35.22839448903653	10.904945607213037	7.743014747544724
11	40.15608853470612	19.77652200125392	26.92166071112706	13.145728752912897
12	37.108217681580285	23.051613998851376	26.029983046695904	13.810185272872433
13	19.91568612406891	39.67044266389986	27.481366935182262	12.932504276848963
14	14.518333227930288	42.012830065357385	29.137243433202986	14.331593273509347
15	13.478053320244094	26.169147169510893	46.546370224078316	13.806429286166702
16	15.991161553334852	22.61415390621012	44.84089544007163	16.5537891003834
17	15.516173696086744	22.94673529314513	29.729694156744067	31.807396854024056
18	19.67873793710295	26.294635648422954	35.60367213510509	18.42295427936901
19	27.488622088989917	26.095793070172423	27.588524914872327	18.82705992596533
20	29.57329101802328	25.982150395486137	26.893282144905967	17.551276441584616
21	21.733070050436158	30.431325314355224	29.874219388788603	17.961385246420015
22	21.560102047269574	26.431424189902685	26.878033480929698	25.130440281898036
23	17.751659937458005	32.40251208095468	26.839029003600935	23.006798977986385
24	17.621131373821157	26.070528441647532	40.54873360648239	15.75960657804892
25	16.273727322427703	28.009805372473494	36.43570344655119	19.280763858547616
26	15.658515961498889	36.71955331366309	28.567585449500115	19.0543452753379
27	16.423870483305603	37.28070414798973	29.102283864634238	17.193141504070432
28	13.875096427735617	31.469422255409025	38.49902424601777	16.156457070837586
29	15.45126254122356	26.962655540385366	37.402532947547485	20.183548970843592
30	17.64697384645462	33.10549154148584	31.642582873278467	17.60495173878108
31	26.76731212923676	28.839942639341487	26.984966743466426	17.407778487955323
32	28.261135457252855	27.698379500402403	28.250766365748998	15.78971867659574
33	26.83270482085709	29.029475506953872	25.85248859801215	18.285331074176884
34	18.75999790692023	28.771403907574477	28.951787576801102	23.516810608704194
35	18.441348983491956	28.096610398561555	32.67560762715701	20.786432990789486
36	28.590378189338328	27.356456300378778	26.858226268985792	17.194939241297106
37	18.814154740873835	34.7480134201084	29.333453610514454	17.104378228503318
38	18.50487973298466	35.14049797963228	25.744142827654464	20.6104794597286
39	19.52689334634981	33.98381458651883	27.710193202177702	18.77909886495366
40	23.301467370908824	27.698828934709073	26.792512552860696	22.207191141521406
41	16.397161244509277	26.812191355002703	28.406687967712642	28.38395943277538
42	19.872251508574404	26.88419715142116	26.76496865035199	26.47858268965245
43	20.384446105924603	27.105896674410847	27.756067603908406	24.753589615756137
44	17.678530555272875	31.595616988231562	30.437424779945726	20.288427676549833
45	17.064506985011686	38.05780816667079	24.92122861214365	19.956456236173874
46	20.368009651280712	34.75761205280083	26.27386536296475	18.600512932953713
47	19.906729540386007	29.526614055030663	27.826660892505906	22.739995512077424
48	21.279077195799587	26.84185401924285	32.1437342276648	19.73533455729276
49	20.461684601770706	27.093408821175547	32.14068449486955	20.3042220821842
50	18.253966980061488	34.987561905562934	27.62476858146013	19.133702532915446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3827.0
1	4435.5
2	5044.0
3	11568.5
4	18093.0
5	13810.5
6	9528.0
7	10454.0
8	11380.0
9	12592.5
10	13805.0
11	14750.0
12	15695.0
13	15651.0
14	15607.0
15	15312.5
16	15018.0
17	14327.0
18	13636.0
19	14058.0
20	14480.0
21	16392.0
22	18304.0
23	23809.5
24	29315.0
25	33937.5
26	38560.0
27	40864.0
28	43168.0
29	55807.0
30	68446.0
31	77321.5
32	86197.0
33	98331.0
34	110465.0
35	121325.5
36	132186.0
37	140447.5
38	148709.0
39	173532.0
40	198355.0
41	247113.0
42	295871.0
43	335883.0
44	375895.0
45	357962.0
46	340029.0
47	304734.0
48	269439.0
49	238764.0
50	208089.0
51	185504.5
52	162920.0
53	141648.5
54	120377.0
55	105961.0
56	91545.0
57	80729.0
58	69913.0
59	65094.5
60	60276.0
61	52287.0
62	44298.0
63	35838.5
64	27379.0
65	21742.0
66	16105.0
67	12771.5
68	9438.0
69	7405.5
70	5373.0
71	4386.5
72	3400.0
73	2678.0
74	1956.0
75	1696.0
76	1436.0
77	1101.0
78	766.0
79	586.5
80	407.0
81	254.5
82	102.0
83	75.0
84	48.0
85	55.0
86	62.0
87	51.0
88	40.0
89	42.5
90	45.0
91	22.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3115027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.046990647330343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00969367901416	13.809714904892505
2	8.505839239110019	2.8999792391360786
3	2.709576506439733	1.3857037609051248
4	1.2455463291367592	0.8493126649444391
5	0.7310022007437909	0.623069383962865
6	0.48505419091859514	0.4961228553622607
7	0.34128109993110745	0.4072471003045341
8	0.26666916932183	0.363672546828847
9	0.20960995084107698	0.3215896984417691
>10	2.013990345710306	7.971285352450537
>50	0.857539743520592	10.77382057715958
>100	1.5911481271504588	55.83480092461717
>500	0.030183861673653382	3.2512016746732986
>1k	0.00267451938880473	0.6844412284052399
>5k	0.0	0.0
>10k+	1.9103709920033785E-4	0.3280380879157816
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10073	0.32336798364829583	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4704	0.1510099270407608	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04019226799639297	0.0
2	0.0	0.0	0.0	0.16359408762749086	0.0
3	0.0	0.0	0.0	0.22699642731828648	0.0
4	0.0	0.0	0.0	0.36619265258374967	0.0
5	0.0	0.0	0.0	0.6039755032620905	0.0
6	0.0	0.0	0.0	0.9156260924865178	0.0
7	0.0	0.0	0.0	1.0827835521168838	0.0
8	0.0	0.0	0.0	1.4951074260351516	0.0
9	0.0	0.0	0.0	1.6532761995321388	0.0
10	0.0	0.0	0.0	1.9206575095496765	0.0
11	0.0	0.0	0.0	2.1934641336977174	0.0
12	0.0	0.0	0.0	2.4486786149847175	0.0
13	0.0	0.0	0.0	2.551149636905234	0.0
14	0.0	0.0	0.0	2.5870080740873194	0.0
15	0.0	0.0	0.0	2.6394955806161553	0.0
16	0.0	0.0	0.0	2.747199301964317	0.0
17	0.0	0.0	0.0	2.884116253245959	0.0
18	0.0	0.0	0.0	3.0698931341526094	0.0
19	0.0	0.0	0.0	3.1632470601378415	0.0
20	0.0	0.0	0.0	3.260613792432618	0.0
21	0.0	0.0	0.0	3.3909176389161315	0.0
22	0.0	0.0	0.0	3.546197191870247	0.0
23	0.0	0.0	0.0	3.7108506603634575	0.0
24	0.0	0.0	0.0	3.8330646893269305	0.0
25	0.0	0.0	0.0	3.9338021789217237	0.0
26	0.0	0.0	0.0	4.039547650790828	0.0
27	0.0	0.0	0.0	4.135501875264644	0.0
28	0.0	0.0	0.0	4.242274625548992	0.0
29	0.0	0.0	0.0	4.364809679017228	0.0
30	0.0	0.0	0.0	4.526927053922807	0.0
31	0.0	0.0	0.0	4.679124771631193	0.0
32	0.0	0.0	0.0	4.809653335268041	0.0
33	0.0	0.0	0.0	4.938159444524879	0.0
34	0.0	0.0	0.0	5.0818179104065555	0.0
35	0.0	0.0	0.0	5.251415156273124	0.0
36	0.0	0.0	0.0	5.405025381802469	0.0
37	6.420490095270442E-5	0.0	0.0	5.556324230897517	0.0
38	6.420490095270442E-5	0.0	0.0	5.702422483015396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGACT	20	7.858557E-4	44.000004	43
TCCGTCG	20	7.858557E-4	44.000004	1
ACGTCGA	40	8.3200575E-9	44.000004	19
TTACGTA	40	8.3200575E-9	44.000004	40
CGTCGGA	20	7.858557E-4	44.000004	12
GTCGACG	25	4.4445995E-5	44.0	32
CGTTGCA	25	4.4445995E-5	44.0	15
GTTCGAC	25	4.4445995E-5	44.0	15
TTAACGC	30	2.5291174E-6	44.0	21
CGTCGGT	30	2.5291174E-6	44.0	32
CGGTCGA	25	4.4445995E-5	44.0	27
TCGACGA	25	4.4445995E-5	44.0	33
CCGTTCG	25	4.4445995E-5	44.0	26
CGCATCG	65	0.0	43.999996	21
TCCCGTA	35	1.4471516E-7	43.999996	38
TCGTTAG	130	0.0	42.30769	1
CTTGCGG	165	0.0	41.333332	2
CGGTCTA	245	0.0	41.30612	31
CCGACAA	75	0.0	41.06667	40
ACGTCGG	70	0.0	40.85714	2
>>END_MODULE