FastQCFastQC Report
Thu 26 May 2016
SRR1547152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547152_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2855571
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76810.26898298098699No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34320.12018612039413484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG207.858447E-444.00000427
TACCGTT207.858447E-444.00000440
ACTATCG351.447097E-744.015
CGTACCA302.5290428E-644.023
CGACTAT254.4445063E-544.043
GCGATAT2150.041.953499
ATCGCAA750.041.06666635
TACGGGA10200.040.117654
ACGGGAT10000.039.825
ATCGACG950.039.3684231
TATAGCG1200.038.51
TGGGCGA12400.038.4999966
GGGCGAT42650.038.4290737
CGTTTTT71650.038.4117241
CGTAAGG3100.038.322582
TAGGGAC19800.038.222225
CCGGATA750.038.1333320
CGGTCTA2850.037.82456231
CCAACGG1400.037.7142872
ACGGTCT2800.037.71428730
Produced by FastQC (version 0.11.5)