##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547152_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2855571
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013677824855343	31.0	31.0	33.0	30.0	34.0
2	31.389468165911477	31.0	31.0	34.0	30.0	34.0
3	31.557572198344918	31.0	31.0	34.0	30.0	34.0
4	35.32949662256691	37.0	35.0	37.0	33.0	37.0
5	35.11692197462434	37.0	35.0	37.0	32.0	37.0
6	35.18381612644196	37.0	35.0	37.0	32.0	37.0
7	35.6846784758635	37.0	35.0	37.0	35.0	37.0
8	35.72559673704489	37.0	35.0	37.0	35.0	37.0
9	37.53268085437203	39.0	37.0	39.0	35.0	39.0
10	36.755248600017296	39.0	37.0	39.0	32.0	39.0
11	36.35773440758433	38.0	35.0	39.0	32.0	39.0
12	35.503227550636986	37.0	35.0	39.0	30.0	39.0
13	35.15414080056143	37.0	34.0	39.0	30.0	39.0
14	36.05324434237496	38.0	35.0	40.0	29.0	41.0
15	36.38721572673206	38.0	35.0	40.0	30.0	41.0
16	36.5414762931827	38.0	35.0	40.0	31.0	41.0
17	36.46044311277849	38.0	35.0	40.0	31.0	41.0
18	36.394791444513196	38.0	35.0	40.0	31.0	41.0
19	36.36072540308051	38.0	35.0	40.0	31.0	41.0
20	36.20701393871838	38.0	34.0	40.0	30.0	41.0
21	36.051186610313664	37.0	34.0	40.0	30.0	41.0
22	35.998202110891306	37.0	34.0	40.0	30.0	41.0
23	35.873971965676915	37.0	34.0	40.0	30.0	41.0
24	35.84976279700277	37.0	34.0	40.0	30.0	41.0
25	35.690866380139035	37.0	34.0	40.0	29.0	41.0
26	35.57666995497573	37.0	34.0	40.0	29.0	41.0
27	35.395478872701815	37.0	34.0	40.0	29.0	41.0
28	35.39986048324486	37.0	34.0	40.0	29.0	41.0
29	35.32414602893782	36.0	34.0	40.0	29.0	41.0
30	35.311858468936684	36.0	34.0	40.0	29.0	41.0
31	35.2234845500252	37.0	34.0	40.0	28.0	41.0
32	35.03682661015958	36.0	34.0	40.0	27.0	41.0
33	34.8978298911146	36.0	33.0	40.0	27.0	41.0
34	34.85225231661198	36.0	34.0	40.0	26.0	41.0
35	34.649854617517825	36.0	33.0	40.0	25.0	41.0
36	34.43316870776458	36.0	33.0	40.0	24.0	41.0
37	34.52615781572232	36.0	33.0	40.0	24.0	41.0
38	34.538920236968366	36.0	33.0	40.0	25.0	41.0
39	34.47213604564551	36.0	33.0	40.0	24.0	41.0
40	34.434665431186964	36.0	33.0	40.0	24.0	41.0
41	34.4835845440369	36.0	33.0	40.0	24.0	41.0
42	34.24364023867731	36.0	33.0	40.0	23.0	41.0
43	34.29093305682121	36.0	33.0	40.0	24.0	41.0
44	34.08546311753411	35.0	33.0	40.0	23.0	41.0
45	33.92322796386432	35.0	33.0	40.0	23.0	41.0
46	34.0076114374323	35.0	33.0	40.0	23.0	41.0
47	33.89600888929044	35.0	33.0	40.0	23.0	41.0
48	33.96188117893059	35.0	33.0	40.0	23.0	41.0
49	33.88981293058376	36.0	33.0	40.0	24.0	41.0
50	33.40117335552154	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	7.0
14	23.0
15	83.0
16	257.0
17	659.0
18	1478.0
19	3012.0
20	5335.0
21	8672.0
22	13239.0
23	18935.0
24	26829.0
25	36924.0
26	48456.0
27	58615.0
28	67639.0
29	78790.0
30	93677.0
31	113372.0
32	139336.0
33	175514.0
34	274495.0
35	361162.0
36	211931.0
37	257209.0
38	359135.0
39	500776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.710921213305504	24.407412738117877	27.80119983008652	13.080466218490102
2	30.30906953460446	26.085956188797265	29.372059038279907	14.23291523831836
3	28.05186773503443	26.951807536916434	30.861498453374125	14.134826274675014
4	25.867786162557334	28.306037566567248	30.47814255012395	15.348033720751472
5	22.957860266825794	32.64383900803027	30.302135719966337	14.0961650051776
6	20.87922170382036	41.359994200809574	27.43629907993883	10.32448501543124
7	88.86019643706985	3.7894697767977057	5.7457160056605145	1.6046177804719266
8	89.80182947648649	3.009590726338095	5.601856861552383	1.586722935623033
9	85.5883464287878	5.068268307809541	6.961514877409806	2.381870385992854
10	46.00144769645021	27.82448764187618	14.194884315606233	11.97918034606739
11	41.668864125598695	23.66412882047058	21.67626019454603	12.990746859384691
12	37.70934079383773	23.436923823641575	23.90225982824451	14.951475554276186
13	22.33119050445603	35.901471194377585	25.409979300111956	16.35735900105443
14	17.253081782942886	38.42590501164215	27.608348733055486	16.712664472359467
15	15.991582769260509	26.667416078955835	41.81149759540211	15.529503556381544
16	18.625451792303537	22.78349934216309	41.21067205122898	17.38037681430439
17	19.27852608112353	22.775479930283645	28.539826185375883	29.40616780321694
18	22.268856211244618	24.916242670905397	34.045625200704166	18.769275917145816
19	28.287757509794016	25.860642232324118	27.157300588918993	18.69429966896288
20	30.33620946563752	25.99714733060393	26.563618974979086	17.10302422877946
21	23.6339772325745	28.933862964710034	29.353989097101767	18.078170705613694
22	23.10774272465997	24.88136348211969	27.503571089634963	24.507322703585377
23	19.839779854887166	30.408384172552527	27.18146388235488	22.570372090205424
24	19.99410975948418	25.30516663742558	36.94980793683645	17.75091566625379
25	18.763147545622225	26.62626844158314	34.95059306877678	19.659990944017853
26	17.947093593540487	34.26060847375184	27.9060124927729	19.88628543993478
27	18.353352096655975	35.07207490200734	28.884976069584685	17.689596931752003
28	16.862546930193645	30.81184113440009	35.551663747810856	16.773948187595405
29	17.221739540007935	27.673344490471436	34.983511178674945	20.121404790845684
30	19.574859108738675	31.70224098787948	30.69354605436181	18.029353849020037
31	27.5473101526805	28.422896856705716	26.242037056686733	17.78775593392705
32	28.78156417753227	26.780423249850905	27.84210233259828	16.595910240018547
33	26.89076895654144	27.42754426347655	26.360087001864073	19.321599778117932
34	19.64909995233878	28.180283382903106	29.082554767505343	23.088061897252775
35	19.9031297068082	27.59973399365661	31.35369423488332	21.143442064651868
36	27.962253433726563	27.455489637624137	26.83193658991494	17.750320338734355
37	20.277835851393643	33.885972367698095	28.014852371031925	17.821339409876344
38	20.02314073087309	34.833558682309075	25.111755232140965	20.031545354676876
39	20.50413034731057	32.72196699013962	26.697462609054373	20.076440053495432
40	23.880127652227873	26.882819583193694	26.9589164478838	22.27813631669463
41	18.71198439821668	26.2199749192018	27.747340199210598	27.320700483370924
42	21.10022829059407	26.551362231931897	26.275410417040934	26.072999060433098
43	21.170406899355683	26.439265561948904	27.609819542221153	24.78050799647426
44	18.5857049255648	30.080498786407343	30.133973205358927	21.19982308266893
45	18.217722480022385	36.2991849966259	25.249906235915688	20.233186287436034
46	21.00872294893035	32.92868571644691	26.479397640611985	19.58319369401076
47	20.513830683950776	29.622832001025362	27.437839927636187	22.425497387387672
48	21.939570054465467	26.996597177937442	31.244188990573164	19.81964377702393
49	21.236033003556905	26.758396131631816	31.86322455298783	20.14234631182345
50	19.715566518920383	33.13666513632475	27.312751110023182	19.835017234731687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2244.0
1	3768.5
2	5293.0
3	10833.0
4	16373.0
5	13077.5
6	9782.0
7	10169.0
8	10556.0
9	11281.0
10	12006.0
11	12265.0
12	12524.0
13	12813.0
14	13102.0
15	12637.0
16	12172.0
17	12249.0
18	12326.0
19	12351.5
20	12377.0
21	12637.5
22	12898.0
23	15755.0
24	18612.0
25	22361.0
26	26110.0
27	33512.5
28	40915.0
29	44919.0
30	48923.0
31	57854.5
32	66786.0
33	78437.5
34	90089.0
35	99539.0
36	108989.0
37	117988.0
38	126987.0
39	141537.5
40	156088.0
41	196523.5
42	236959.0
43	266618.0
44	296277.0
45	296656.0
46	297035.0
47	263921.5
48	230808.0
49	216724.0
50	202640.0
51	184065.5
52	165491.0
53	149687.0
54	133883.0
55	119337.0
56	104791.0
57	96465.0
58	88139.0
59	83873.5
60	79608.0
61	72944.5
62	66281.0
63	58255.0
64	50229.0
65	41056.5
66	31884.0
67	26333.5
68	20783.0
69	17931.0
70	15079.0
71	12131.5
72	9184.0
73	7402.0
74	5620.0
75	4227.5
76	2835.0
77	2105.5
78	1376.0
79	1084.0
80	792.0
81	602.5
82	413.0
83	297.0
84	181.0
85	133.5
86	86.0
87	52.0
88	18.0
89	15.0
90	12.0
91	8.5
92	5.0
93	5.0
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2855571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.786541453191106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77749782497565	13.895478986303903
2	7.048988109385333	2.366562622024962
3	2.019867243811753	1.0171975565456657
4	0.9604994914912232	0.6449385811874558
5	0.5465669909855985	0.4587484725562838
6	0.3549625061786329	0.35751556945777346
7	0.26168242443986933	0.3074920005801991
8	0.22347363355711913	0.30010795307214555
9	0.18486755289174098	0.27929581559704886
>10	2.558505987268367	10.855837749731348
>50	1.3999601836010405	17.343785853113793
>100	1.6485373542208053	50.20243862806998
>500	0.013321940915339302	1.3965473139016427
>1k	0.0010572968980428017	0.30140261907680777
>5k	2.1145937960856032E-4	0.27265027878093984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7681	0.26898298098699	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3432	0.12018612039413484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016143881556438276	0.0
2	0.0	0.0	0.0	0.07045876288840305	0.0
3	0.0	0.0	0.0	0.09808896364334839	0.0
4	0.0	0.0	0.0	0.15947773667683277	0.0
5	0.0	0.0	0.0	0.26572618926302305	0.0
6	0.0	0.0	0.0	0.4047526746839774	0.0
7	0.0	0.0	0.0	0.477347612789176	0.0
8	0.0	0.0	0.0	0.6862025143132494	0.0
9	0.0	0.0	0.0	0.758447259759957	0.0
10	0.0	0.0	0.0	0.8899446030233533	0.0
11	0.0	0.0	0.0	1.0451850085324441	0.0
12	0.0	0.0	0.0	1.167647381206771	0.0
13	0.0	0.0	0.0	1.2202112992462804	0.0
14	0.0	0.0	0.0	1.2411178009581971	0.0
15	0.0	0.0	0.0	1.2749814310342835	0.0
16	0.0	0.0	0.0	1.3469460223541982	0.0
17	0.0	0.0	0.0	1.4263346980341234	0.0
18	0.0	0.0	0.0	1.5359800194076771	0.0
19	0.0	0.0	0.0	1.5946372897049312	0.0
20	0.0	0.0	0.0	1.6567614673212467	0.0
21	0.0	0.0	0.0	1.7407726860932542	0.0
22	0.0	0.0	0.0	1.8300367947426277	0.0
23	0.0	0.0	0.0	1.9344292262388152	0.0
24	0.0	0.0	0.0	2.017529944098746	0.0
25	0.0	0.0	0.0	2.0855023391118626	0.0
26	0.0	0.0	0.0	2.1572568148366824	0.0
27	0.0	0.0	0.0	2.2274704428641416	0.0
28	0.0	0.0	0.0	2.3060186561636886	0.0
29	7.003853169821377E-5	0.0	0.0	2.391990953823246	0.0
30	7.003853169821377E-5	0.0	0.0	2.498589599067927	0.0
31	7.003853169821377E-5	0.0	0.0	2.601441182866754	0.0
32	7.003853169821377E-5	0.0	0.0	2.692106062150092	0.0
33	7.003853169821377E-5	0.0	0.0	2.788479081766834	0.0
34	7.003853169821377E-5	0.0	0.0	2.88901939401962	0.0
35	7.003853169821377E-5	0.0	0.0	3.0112016125671537	0.0
36	7.003853169821377E-5	0.0	0.0	3.1132477532514513	0.0
37	7.003853169821377E-5	0.0	0.0	3.225239365436895	0.0
38	7.003853169821377E-5	0.0	0.0	3.339507229902531	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATG	20	7.858447E-4	44.000004	27
TACCGTT	20	7.858447E-4	44.000004	40
ACTATCG	35	1.447097E-7	44.0	15
CGTACCA	30	2.5290428E-6	44.0	23
CGACTAT	25	4.4445063E-5	44.0	43
GCGATAT	215	0.0	41.95349	9
ATCGCAA	75	0.0	41.066666	35
TACGGGA	1020	0.0	40.11765	4
ACGGGAT	1000	0.0	39.82	5
ATCGACG	95	0.0	39.368423	1
TATAGCG	120	0.0	38.5	1
TGGGCGA	1240	0.0	38.499996	6
GGGCGAT	4265	0.0	38.429073	7
CGTTTTT	7165	0.0	38.411724	1
CGTAAGG	310	0.0	38.32258	2
TAGGGAC	1980	0.0	38.22222	5
CCGGATA	75	0.0	38.13333	20
CGGTCTA	285	0.0	37.824562	31
CCAACGG	140	0.0	37.714287	2
ACGGTCT	280	0.0	37.714287	30
>>END_MODULE