FastQCFastQC Report
Thu 26 May 2016
SRR1547151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547151_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3361347
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85290.25373756413723425No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43730.13009665470420043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC207.858646E-444.0000049
GTCGAAC351.4472062E-744.012
CCGTCTA351.4472062E-744.034
GCGTAAG1450.040.9655151
ACGTAAG2050.040.7804871
ATCGCTA603.6379788E-1240.33333231
TTAGACG1150.040.1739161
AACGTAG1650.040.01
TAAGACG1100.040.01
AATCACG1000.039.61
TAATCCG950.039.368421
TCGAATA452.3532266E-839.11110723
TAGGGAC24750.038.9333345
CGTAAGG4550.038.681322
GGTACGA1200.038.58
TTAACGG2250.038.1333352
GGGCGAT44200.037.92767
CGTTTTT82700.037.8016931
CGTCTAC700.037.71428713
GGCGATT13600.037.2058838
Produced by FastQC (version 0.11.5)