FastQCFastQC Report
Thu 26 May 2016
SRR1547150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547150_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3501833
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT708062.021969637044371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.858694E-444.01
CGTTTTT463600.043.1837771
TCACGAC2550.039.68627525
TACGGGA18150.039.1515164
CGTTCGA850.038.82353214
GTTTTTT525400.038.4727822
TAGGGCG10350.038.048315
ACGGGAT29100.037.951895
CTATGCG1350.037.481481
AGGGATT122450.037.4242556
TATGGGA61500.037.2390254
CGAACGG1300.037.2307662
AGGGATC57600.037.201396
GACCGAT18100.037.193379
TGGGCGA16050.037.146426
TAAGGGA61750.037.0170024
TAGGGAT92400.037.05
AGGGCGA26400.036.9166686
AAGGGAT85800.036.769235
CGTTGCG301.3016394E-436.6666641
Produced by FastQC (version 0.11.5)