##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547150_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3501833
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092785692521602	31.0	31.0	33.0	30.0	34.0
2	31.481407594251355	31.0	31.0	34.0	30.0	34.0
3	31.499961020414165	31.0	31.0	34.0	30.0	34.0
4	35.33283169128853	37.0	35.0	37.0	33.0	37.0
5	35.177758333992514	37.0	35.0	37.0	32.0	37.0
6	35.24802610518548	37.0	35.0	37.0	32.0	37.0
7	35.77969109320747	37.0	35.0	37.0	35.0	37.0
8	35.833226198964944	37.0	35.0	37.0	35.0	37.0
9	37.61479373802234	39.0	37.0	39.0	35.0	39.0
10	36.95001846175988	39.0	37.0	39.0	32.0	39.0
11	36.596536156921246	39.0	35.0	39.0	32.0	39.0
12	36.372290740306575	38.0	35.0	39.0	32.0	39.0
13	36.33424095323792	38.0	35.0	39.0	32.0	39.0
14	37.408071715584384	39.0	36.0	41.0	32.0	41.0
15	37.528345012454906	39.0	36.0	41.0	32.0	41.0
16	37.50718637924767	39.0	36.0	41.0	32.0	41.0
17	37.38223039191189	39.0	36.0	41.0	32.0	41.0
18	37.14288431230159	39.0	36.0	40.0	32.0	41.0
19	36.96747760387203	38.0	36.0	40.0	31.0	41.0
20	36.69219605846424	38.0	35.0	40.0	31.0	41.0
21	36.60170173734727	38.0	35.0	40.0	31.0	41.0
22	36.54144843571924	38.0	35.0	40.0	31.0	41.0
23	36.35094649002394	38.0	35.0	40.0	30.0	41.0
24	36.2740387676968	38.0	35.0	40.0	30.0	41.0
25	36.142296620084394	38.0	35.0	40.0	30.0	41.0
26	36.04725696513797	38.0	35.0	40.0	30.0	41.0
27	35.89621606741384	38.0	35.0	40.0	30.0	41.0
28	35.822976709626076	38.0	34.0	40.0	30.0	41.0
29	35.664145320465025	38.0	34.0	40.0	29.0	41.0
30	35.511643188010396	38.0	34.0	40.0	28.0	41.0
31	35.29333523329068	38.0	34.0	40.0	27.0	41.0
32	34.86961571268533	38.0	34.0	40.0	24.0	41.0
33	34.39642867035635	38.0	33.0	40.0	22.0	41.0
34	34.05397259092595	38.0	33.0	40.0	20.0	41.0
35	33.701067983538906	37.0	33.0	40.0	18.0	41.0
36	33.48691899356708	37.0	33.0	40.0	16.0	41.0
37	33.49518637810541	37.0	33.0	40.0	16.0	41.0
38	33.40453356856252	37.0	32.0	40.0	15.0	41.0
39	33.3002995859597	37.0	32.0	40.0	15.0	41.0
40	33.31400183846574	37.0	32.0	40.0	15.0	41.0
41	33.29900911893857	37.0	32.0	40.0	15.0	41.0
42	33.054386088657	37.0	32.0	40.0	13.0	41.0
43	33.09892761876423	37.0	32.0	40.0	14.0	41.0
44	32.9126908678969	37.0	31.0	40.0	12.0	41.0
45	32.756427276800466	37.0	31.0	40.0	10.0	41.0
46	32.8268903742697	37.0	31.0	40.0	12.0	41.0
47	32.70151403564933	37.0	31.0	40.0	10.0	41.0
48	32.72324779622558	37.0	31.0	40.0	11.0	41.0
49	32.63304218105204	36.0	31.0	40.0	10.0	41.0
50	32.20868071093053	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	8.0
14	24.0
15	90.0
16	274.0
17	909.0
18	2345.0
19	4853.0
20	8569.0
21	13534.0
22	21123.0
23	30792.0
24	45058.0
25	67665.0
26	94550.0
27	108145.0
28	103904.0
29	100935.0
30	106189.0
31	120198.0
32	143119.0
33	174490.0
34	235512.0
35	298958.0
36	293057.0
37	347892.0
38	484237.0
39	695398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.172721828825075	22.185381198932102	25.79871741456546	22.843179557677367
2	38.13168703361925	23.38438183659815	26.898569977494645	11.585361152287959
3	24.711543925709766	23.45425952636805	39.875916412918606	11.958280135003582
4	22.405951397453848	25.874791859006415	38.82855064761797	12.890706095921765
5	20.420791054284997	29.573683268162704	37.553818243188644	12.45170743436366
6	18.844787858244523	37.14094875455226	34.579490226975416	9.4347731602278
7	80.86345065569945	2.733682617075115	15.209548827713942	1.1933178995114844
8	81.61654196530789	2.3091620873982284	14.855448560796589	1.2188473864973002
9	76.63220947429532	5.777088741810361	15.931427912181992	1.659273871712329
10	29.371931785439227	42.77619749428371	20.615717539928376	7.236153180348692
11	19.937558415835362	22.295095168730207	42.62710414802762	15.140242267406812
12	21.535378757353648	22.40340987134452	40.59454005944886	15.466671311852965
13	18.75677680803168	25.70045459049589	40.20403028927993	15.3387383121925
14	15.706745581528303	29.595443300694235	38.71572402224778	15.982087095529685
15	15.479321829453319	27.755920970531715	42.07119528544051	14.693561914574452
16	18.890192650534736	27.20369589297948	40.15951074765701	13.746600708828776
17	19.23741080742571	26.44118094723535	36.58609648147127	17.735311763867667
18	18.830024161631922	27.10169217092877	38.58876194267402	15.479521724765288
19	20.665805593813296	27.63124340880904	36.05697359068808	15.645977406689582
20	22.168047419737036	27.923518911381557	35.59184575620825	14.316587912673162
21	21.523642046893727	26.20339119541109	37.36857240193922	14.90439435575597
22	20.432413538852366	24.60237252890129	37.74177694938622	17.223436982860118
23	17.836601574089915	27.90584245450882	37.478457710576144	16.77909826082512
24	17.680083544817816	27.432661694603937	39.4946303835734	15.392624377004843
25	18.01156708500948	29.198394098176582	37.21642351305731	15.573615303756632
26	17.52787754298963	31.21242503568845	35.52048312983514	15.739214291486773
27	16.92185207004446	30.089984302506718	37.461238157273634	15.52692547017519
28	16.40058220937435	29.047530250585908	39.23291030725908	15.31897723278066
29	17.09356214302624	26.930267662678375	39.06499824520473	16.91117194909066
30	17.894771109873027	28.065815817030682	37.85520326069233	16.18420981240396
31	20.25516351008172	28.089603359155046	35.321929972103185	16.33330315866005
32	19.742289252514325	29.015147210046855	35.45408932978814	15.78847420765068
33	19.653278725741632	28.990303078416364	34.52865970478889	16.827758491053114
34	18.359356371363226	29.554236309955385	34.25000563990345	17.836401678777943
35	18.66254044667464	29.951513964258147	33.31235384440092	18.07359174466629
36	20.674429648701125	29.902710951664456	32.24408474076291	17.17877465887151
37	19.11321870574639	31.84734965944978	32.19305432326441	16.846377311539413
38	18.745982461185328	32.361537514781546	31.14300424948877	17.74947577454436
39	19.17244483103563	31.753684427555513	31.28647197053657	17.787398770872283
40	20.038991008423306	30.751609228652537	31.50649959606869	17.70290016685547
41	18.42523615489374	30.54017710153511	31.58891357754639	19.445673166024765
42	19.338357939970297	30.810149998586457	30.576957838937496	19.274534222505757
43	19.64788155231846	30.61345301160849	30.697437599108813	19.041227836964243
44	18.86631943899095	31.720987265811935	31.235641448350048	18.177051846847064
45	18.664168165643535	32.29520082768082	30.28448244105301	18.75614856562263
46	19.723356310823505	31.81922153340836	30.514647614549297	17.942774541218842
47	19.113561383424052	30.831253232235806	30.726022628720447	19.3291627556197
48	19.836382831505674	30.011739566107238	31.67889502440579	18.472982577981302
49	19.439904758450787	30.069480754793275	31.640943471604725	18.84967101515121
50	18.42249473347244	31.335960338485584	31.723100444824183	18.518444483217788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2556.0
1	3206.0
2	3856.0
3	56179.0
4	108502.0
5	77004.5
6	45507.0
7	46165.0
8	46823.0
9	49144.5
10	51466.0
11	52826.5
12	54187.0
13	53719.5
14	53252.0
15	51216.5
16	49181.0
17	46689.0
18	44197.0
19	42631.5
20	41066.0
21	41209.5
22	41353.0
23	44370.0
24	47387.0
25	52872.0
26	58357.0
27	66157.5
28	73958.0
29	86809.5
30	99661.0
31	110076.0
32	120491.0
33	133538.5
34	146586.0
35	159419.0
36	172252.0
37	179175.5
38	186099.0
39	196788.0
40	207477.0
41	222367.5
42	237258.0
43	247302.0
44	257346.0
45	263429.5
46	269513.0
47	251677.0
48	233841.0
49	216657.5
50	199474.0
51	180144.5
52	160815.0
53	143760.0
54	126705.0
55	112888.0
56	99071.0
57	89923.5
58	80776.0
59	71815.0
60	62854.0
61	54946.5
62	47039.0
63	39543.0
64	32047.0
65	24964.5
66	17882.0
67	14063.0
68	10244.0
69	7895.0
70	5546.0
71	4492.5
72	3439.0
73	2661.5
74	1884.0
75	1426.0
76	968.0
77	774.0
78	580.0
79	381.0
80	182.0
81	131.5
82	81.0
83	56.5
84	32.0
85	27.0
86	22.0
87	16.0
88	10.0
89	8.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3501833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.50972172335037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.64091648452079	19.274669791048417
2	6.7015173224577715	3.2850464939530406
3	2.6949490242097265	1.9815735192598505
4	1.5808808196913888	1.549877958736719
5	1.081607972701899	1.3254955212340345
6	0.7757605973547254	1.1408205819062625
7	0.6069266891732248	1.0412922980677075
8	0.48316086548161596	0.9473710688454025
9	0.40688620172789114	0.8975400819679454
>10	5.298939693187718	30.830046501492863
>50	1.380353928579105	23.496137629967997
>100	0.34476639158216127	11.33281295114075
>500	0.002143291713436928	0.349071707646859
>1k	0.0010716458567183664	0.4818304400342142
>5k	0.0	0.0
>10k+	1.1907176185759628E-4	2.0664134546979147
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70806	2.021969637044371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013964115364724703	0.0
2	0.0	0.0	0.0	0.05065918334769248	0.0
3	0.0	0.0	0.0	0.0979201463919039	0.0
4	0.0	0.0	0.0	0.1529199136566478	0.0
5	0.0	0.0	0.0	0.295273932252052	0.0
6	0.0	0.0	0.0	0.6266146900780248	0.0
7	0.0	0.0	0.0	0.8822236811407055	0.0
8	0.0	0.0	0.0	1.227528554331403	0.0
9	0.0	0.0	0.0	1.445014653754191	0.0
10	0.0	0.0	0.0	1.6425683349263085	0.0
11	0.0	0.0	0.0	1.7846653452634662	0.0
12	0.0	0.0	0.0	1.9042312982943503	0.0
13	0.0	0.0	0.0	1.98533168200768	0.0
14	0.0	0.0	0.0	2.038046931421344	0.0
15	0.0	0.0	0.0	2.077340638459915	0.0
16	0.0	0.0	0.0	2.1302272267124103	0.0
17	0.0	0.0	0.0	2.1907669497660227	0.0
18	0.0	0.0	0.0	2.268983129692364	0.0
19	0.0	0.0	0.0	2.323297541601784	0.0
20	0.0	0.0	0.0	2.383922934074812	0.0
21	0.0	0.0	0.0	2.4512305412622477	0.0
22	0.0	0.0	0.0	2.5268195256598474	0.0
23	0.0	0.0	0.0	2.607234554017853	0.0
24	0.0	0.0	0.0	2.6745992741515656	0.0
25	0.0	0.0	0.0	2.739679476434199	0.0
26	0.0	0.0	0.0	2.801846918456705	0.0
27	2.8556473138496324E-5	0.0	0.0	2.8695828727412187	0.0
28	2.8556473138496324E-5	0.0	0.0	2.942744556922046	0.0
29	2.8556473138496324E-5	0.0	0.0	3.0285853151763664	0.0
30	2.8556473138496324E-5	0.0	0.0	3.133016337443847	0.0
31	2.8556473138496324E-5	0.0	0.0	3.222769332518141	0.0
32	2.8556473138496324E-5	0.0	0.0	3.321089269533984	0.0
33	2.8556473138496324E-5	0.0	0.0	3.413155338932496	0.0
34	2.8556473138496324E-5	0.0	0.0	3.510390129969076	0.0
35	5.711294627699265E-5	0.0	0.0	3.614021570988679	0.0
36	5.711294627699265E-5	0.0	0.0	3.71693910017982	0.0
37	5.711294627699265E-5	0.0	0.0	3.820856105930808	0.0
38	5.711294627699265E-5	0.0	0.0	3.936766830399965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.858694E-4	44.0	1
CGTTTTT	46360	0.0	43.183777	1
TCACGAC	255	0.0	39.686275	25
TACGGGA	1815	0.0	39.151516	4
CGTTCGA	85	0.0	38.823532	14
GTTTTTT	52540	0.0	38.472782	2
TAGGGCG	1035	0.0	38.04831	5
ACGGGAT	2910	0.0	37.95189	5
CTATGCG	135	0.0	37.48148	1
AGGGATT	12245	0.0	37.424255	6
TATGGGA	6150	0.0	37.239025	4
CGAACGG	130	0.0	37.230766	2
AGGGATC	5760	0.0	37.20139	6
GACCGAT	1810	0.0	37.19337	9
TGGGCGA	1605	0.0	37.14642	6
TAAGGGA	6175	0.0	37.017002	4
TAGGGAT	9240	0.0	37.0	5
AGGGCGA	2640	0.0	36.916668	6
AAGGGAT	8580	0.0	36.76923	5
CGTTGCG	30	1.3016394E-4	36.666664	1
>>END_MODULE