FastQCFastQC Report
Thu 26 May 2016
SRR1547149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547149_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3402291
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93700.27540266250006246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43730.1285310398199331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC254.444686E-544.026
TTAACGC207.858661E-444.024
CGGACCG207.858661E-444.024
CGCATTA351.4472062E-743.99999643
GTATCGA351.4472062E-743.99999614
CGAATAT1600.041.2514
GCGTAAG2300.041.1304321
AATACGG5450.040.770642
TAATACG1600.039.8751
TACGTAA452.3532266E-839.1111121
CGAAAGG5450.038.7522932
CGTTTTT82100.038.721071
CGTAAGG3900.038.3589742
CCGCTCG1900.038.21052619
TAACGGG12900.038.201553
ATTCGTC357.2944204E-637.71428327
GTACGAT357.2944204E-637.71428323
ATAACGG6950.037.6690642
GACCGAT17250.037.6231889
GGCGATA13100.037.4503828
Produced by FastQC (version 0.11.5)