##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547149_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3402291
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.005970976615462	31.0	31.0	33.0	30.0	34.0
2	31.369421957145935	31.0	31.0	34.0	30.0	34.0
3	31.5317331762627	31.0	31.0	34.0	30.0	34.0
4	35.29710803690807	37.0	35.0	37.0	33.0	37.0
5	35.07683117052598	37.0	35.0	37.0	32.0	37.0
6	35.14884823197075	37.0	35.0	37.0	32.0	37.0
7	35.68694006479751	37.0	35.0	37.0	35.0	37.0
8	35.735156105106824	37.0	35.0	37.0	35.0	37.0
9	37.530707396868756	39.0	37.0	39.0	35.0	39.0
10	36.74966074330503	39.0	37.0	39.0	32.0	39.0
11	36.33549481805054	38.0	35.0	39.0	32.0	39.0
12	35.59722051993789	37.0	35.0	39.0	30.0	39.0
13	35.278088499778534	37.0	35.0	39.0	30.0	39.0
14	36.17592381133771	38.0	35.0	40.0	30.0	41.0
15	36.48068198751959	38.0	35.0	40.0	31.0	41.0
16	36.6099490020107	38.0	35.0	40.0	31.0	41.0
17	36.54687620782585	38.0	35.0	40.0	31.0	41.0
18	36.48572476604735	38.0	35.0	40.0	31.0	41.0
19	36.47236171156435	38.0	35.0	40.0	31.0	41.0
20	36.32040498593448	38.0	35.0	40.0	30.0	41.0
21	36.16789334010524	38.0	34.0	40.0	30.0	41.0
22	36.100625725430305	38.0	34.0	40.0	30.0	41.0
23	35.97757716785542	38.0	34.0	40.0	30.0	41.0
24	35.965756897337705	37.0	34.0	40.0	30.0	41.0
25	35.8120481169894	37.0	34.0	40.0	30.0	41.0
26	35.688358814692805	37.0	34.0	40.0	29.0	41.0
27	35.517507467762165	37.0	34.0	40.0	29.0	41.0
28	35.52777672456589	37.0	34.0	40.0	29.0	41.0
29	35.43537545730215	37.0	34.0	40.0	29.0	41.0
30	35.426820045669224	37.0	34.0	40.0	29.0	41.0
31	35.34186611315728	37.0	34.0	40.0	29.0	41.0
32	35.17915134243367	37.0	34.0	40.0	27.0	41.0
33	35.00908975746049	37.0	34.0	40.0	27.0	41.0
34	34.960808760920216	37.0	34.0	40.0	27.0	41.0
35	34.76983156349648	37.0	33.0	40.0	26.0	41.0
36	34.53434377012431	37.0	33.0	40.0	24.0	41.0
37	34.61940292585202	37.0	33.0	40.0	25.0	41.0
38	34.6384186420268	37.0	33.0	40.0	25.0	41.0
39	34.567146078921525	37.0	33.0	40.0	24.0	41.0
40	34.55154570846527	37.0	33.0	40.0	24.0	41.0
41	34.60681405558784	37.0	33.0	40.0	24.0	41.0
42	34.368044649913834	36.0	33.0	40.0	24.0	41.0
43	34.43567114041686	36.0	33.0	40.0	24.0	41.0
44	34.22935663057628	36.0	33.0	40.0	23.0	41.0
45	33.991426953191244	36.0	33.0	40.0	23.0	41.0
46	34.11126737836358	36.0	33.0	40.0	23.0	41.0
47	33.98623780270412	35.0	33.0	40.0	23.0	41.0
48	34.06461381463255	35.0	33.0	40.0	23.0	41.0
49	34.012349325792535	36.0	33.0	40.0	24.0	41.0
50	33.505476162973714	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	7.0
13	9.0
14	27.0
15	79.0
16	244.0
17	681.0
18	1710.0
19	3460.0
20	6036.0
21	9745.0
22	14998.0
23	21890.0
24	30582.0
25	41659.0
26	55441.0
27	68512.0
28	79310.0
29	92575.0
30	110410.0
31	134526.0
32	165458.0
33	207593.0
34	313761.0
35	413502.0
36	262878.0
37	317216.0
38	436311.0
39	613666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.34041679562389	23.196604875949763	30.92087067214415	12.542107656282193
2	29.886391258125776	25.81563422999385	30.81167954181462	13.48629497006576
3	27.71579503340543	26.011619817352482	32.066128382316506	14.20645676692558
4	25.151111412868566	28.736283874600964	31.502419987002877	14.610184725527592
5	22.397584451183043	32.907296877310024	30.358573090896694	14.336545580610242
6	21.12132677657496	40.46508661369648	28.109382765906854	10.304203843821707
7	89.46789090057258	3.5703589140376293	5.520662400717634	1.441087784672152
8	90.38239233504719	2.690128504587056	5.249286436698095	1.6781927236676697
9	85.5023570882091	5.244172235708233	6.898381120251031	2.3550895558316443
10	44.00179173386403	32.10845280430157	13.512982869484121	10.376772592350273
11	36.831946473714325	22.9122670576973	26.053679711700145	14.202106756888227
12	34.34541607405128	23.133794258045533	27.342017481749796	15.178772186153388
13	20.976395023235813	35.17570954395141	28.287880137236936	15.560015295575836
14	16.563603759937052	38.048832389704465	29.676356314024872	15.711207536333605
15	15.266830497450101	25.85645966203361	43.65093991078365	15.22576992973264
16	17.986468529587857	23.23193401152341	42.07132781998953	16.7102696388992
17	18.75515645193195	22.84128547499317	29.550852646055258	28.852705427019615
18	20.600942129876604	25.654772034490875	35.39647255334714	18.347813282285376
19	27.10791052264489	25.837090360583503	28.065559353976482	18.98943976279513
20	28.87936393447827	25.652979124948455	27.672059797354194	17.795597143219084
21	22.791084007805328	28.687522613439004	29.89947068019755	18.62192269855812
22	22.76369070135388	24.61461997224811	28.266335830768153	24.355353495629856
23	18.626096356837202	30.319863879956184	28.403449322823942	22.650590440382672
24	18.555085382173363	25.817250787778	38.40973626300631	17.217927567042327
25	18.37279644804045	27.476456305471814	34.915326172864106	19.235421073623627
26	17.598406485512264	34.52373709362309	28.337993428545648	19.539862992318998
27	17.722234811778296	34.89630957493054	29.88953619781494	17.491919415476218
28	16.016060942464943	30.07749778017224	36.09459038042308	17.811850896939738
29	16.71362032230635	26.816871337578117	36.231204209163764	20.23830413095176
30	19.049046657090766	31.195127048215454	31.130200209211967	18.625626085481812
31	26.299484670770372	28.108883102591754	27.3333468536348	18.258285373003076
32	27.449004215100942	26.947489206537593	28.659776603471016	16.94372997489045
33	26.461022881346718	27.565954822794403	26.85781433745673	19.11520795840215
34	19.983299488491728	28.354100222467743	28.666301618527047	22.996298670513486
35	19.324566887429675	27.269125421664402	31.963668010760983	21.442639680144936
36	27.913485354427355	26.402679841318687	27.885122113305417	17.79871269094854
37	19.715685695315305	33.11324633901098	29.482751475402896	17.688316490270818
38	19.571782660566072	33.14431364042641	26.695600111807018	20.588303587200507
39	19.96628154381856	31.878490111516033	28.549262834954447	19.605965509710956
40	24.13999860682111	26.882092096178724	27.136068020048842	21.841841276951325
41	18.175341262696225	26.351243911822948	28.78666169354708	26.68675313193375
42	20.696142687383297	26.921536106112026	27.687020304847525	24.69530090165715
43	21.228578037563512	26.412526147822156	28.19500154454748	24.163894270066848
44	18.18603993603134	29.96657252421971	31.065273370208484	20.782114169540467
45	18.203028488744792	35.749146677929666	25.629259813460987	20.418565019864555
46	21.180081304038957	32.24853488428827	27.454118416090807	19.117265395581978
47	20.416977854040116	28.499002583847176	28.202966765629395	22.88105279648331
48	21.23104696217931	26.818576071241406	32.038852643703905	19.91152432287538
49	20.7936064257878	26.264155535196725	32.82332404841326	20.118913990602213
50	20.060159463138223	32.437495793275765	27.760970475482548	19.741374268103463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2996.0
1	4494.0
2	5992.0
3	12514.5
4	19037.0
5	14747.0
6	10457.0
7	11163.0
8	11869.0
9	13100.0
10	14331.0
11	14536.0
12	14741.0
13	14745.5
14	14750.0
15	13946.0
16	13142.0
17	13737.0
18	14332.0
19	15464.0
20	16596.0
21	18560.0
22	20524.0
23	22987.5
24	25451.0
25	31792.5
26	38134.0
27	46391.0
28	54648.0
29	64110.0
30	73572.0
31	85958.5
32	98345.0
33	109848.5
34	121352.0
35	133419.5
36	145487.0
37	151760.5
38	158034.0
39	179402.0
40	200770.0
41	251477.0
42	302184.0
43	317340.0
44	332496.0
45	332011.0
46	331526.0
47	307438.5
48	283351.0
49	268292.0
50	253233.0
51	219203.5
52	185174.0
53	165273.5
54	145373.0
55	133029.5
56	120686.0
57	107519.0
58	94352.0
59	88796.0
60	83240.0
61	74713.0
62	66186.0
63	59180.5
64	52175.0
65	40595.0
66	29015.0
67	24692.0
68	20369.0
69	16076.5
70	11784.0
71	10110.0
72	8436.0
73	6020.0
74	3604.0
75	2999.0
76	2394.0
77	1859.5
78	1325.0
79	869.5
80	414.0
81	337.0
82	260.0
83	181.0
84	102.0
85	61.0
86	20.0
87	16.0
88	12.0
89	11.0
90	10.0
91	7.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3402291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.866330042661364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.60082209920596	13.763063972777026
2	8.152325314233094	2.7499961872999674
3	2.4737469345394305	1.2516909671989151
4	1.107831681049357	0.7474021905717924
5	0.6725013653266604	0.5671314990869918
6	0.4333247657451138	0.43851591088296055
7	0.33063839868116474	0.39036594498535016
8	0.24429318318499493	0.3296263563816364
9	0.20469676014790472	0.31072348037862474
>10	2.1261136408107886	8.613888597351071
>50	0.9829278489919802	12.199485532684665
>100	1.6441485630217159	55.19703485179801
>500	0.024160489890520446	2.5532030432669184
>1k	0.0022926012304873415	0.6091660840898726
>5k	1.7635394080671858E-4	0.2787053812461966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9370	0.27540266250006246	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4373	0.1285310398199331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03374196974920723	0.0
2	0.0	0.0	0.0	0.1196546679869535	0.0
3	0.0	0.0	0.0	0.16562369297629156	0.0
4	0.0	0.0	0.0	0.26552696403687986	0.0
5	0.0	0.0	0.0	0.4383516871425754	0.0
6	0.0	0.0	0.0	0.6602609829670655	0.0
7	0.0	0.0	0.0	0.7822082238115434	0.0
8	2.9391959711852984E-5	0.0	0.0	1.0938511726363207	0.0
9	2.9391959711852984E-5	0.0	0.0	1.2129767853484608	0.0
10	2.9391959711852984E-5	0.0	0.0	1.4176330008220932	0.0
11	2.9391959711852984E-5	0.0	0.0	1.6462730554205975	0.0
12	2.9391959711852984E-5	0.0	0.0	1.826916039809646	0.0
13	2.9391959711852984E-5	0.0	0.0	1.9025709441079555	0.0
14	2.9391959711852984E-5	0.0	0.0	1.9323743912557745	0.0
15	2.9391959711852984E-5	0.0	0.0	1.9764035469041301	0.0
16	2.9391959711852984E-5	0.0	0.0	2.0814798028740045	0.0
17	5.878391942370597E-5	0.0	0.0	2.1998706165933486	0.0
18	5.878391942370597E-5	0.0	0.0	2.3629959929941324	0.0
19	5.878391942370597E-5	0.0	0.0	2.4449407766707787	0.0
20	5.878391942370597E-5	0.0	0.0	2.529413268882644	0.0
21	5.878391942370597E-5	0.0	0.0	2.6467165800926495	0.0
22	5.878391942370597E-5	0.0	0.0	2.774336469161515	0.0
23	5.878391942370597E-5	0.0	0.0	2.9150063883424435	0.0
24	5.878391942370597E-5	0.0	0.0	3.020846835264826	0.0
25	5.878391942370597E-5	0.0	0.0	3.1067007495831485	0.0
26	5.878391942370597E-5	0.0	0.0	3.1974631211733504	0.0
27	5.878391942370597E-5	0.0	0.0	3.2883430606024	0.0
28	5.878391942370597E-5	0.0	0.0	3.384954432175261	0.0
29	5.878391942370597E-5	0.0	0.0	3.4983486127435897	0.0
30	5.878391942370597E-5	0.0	0.0	3.6395475871993312	0.0
31	5.878391942370597E-5	0.0	0.0	3.7674907878250274	0.0
32	5.878391942370597E-5	0.0	0.0	3.8807674005545088	0.0
33	5.878391942370597E-5	0.0	0.0	3.9982764554824968	0.0
34	5.878391942370597E-5	0.0	0.0	4.127894997811769	0.0
35	5.878391942370597E-5	0.0	0.0	4.283437248606894	0.0
36	5.878391942370597E-5	0.0	0.0	4.418023032127469	0.0
37	5.878391942370597E-5	0.0	0.0	4.555606795538653	0.0
38	5.878391942370597E-5	0.0	0.0	4.702831121735325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACC	25	4.444686E-5	44.0	26
TTAACGC	20	7.858661E-4	44.0	24
CGGACCG	20	7.858661E-4	44.0	24
CGCATTA	35	1.4472062E-7	43.999996	43
GTATCGA	35	1.4472062E-7	43.999996	14
CGAATAT	160	0.0	41.25	14
GCGTAAG	230	0.0	41.130432	1
AATACGG	545	0.0	40.77064	2
TAATACG	160	0.0	39.875	1
TACGTAA	45	2.3532266E-8	39.11111	21
CGAAAGG	545	0.0	38.752293	2
CGTTTTT	8210	0.0	38.72107	1
CGTAAGG	390	0.0	38.358974	2
CCGCTCG	190	0.0	38.210526	19
TAACGGG	1290	0.0	38.20155	3
ATTCGTC	35	7.2944204E-6	37.714283	27
GTACGAT	35	7.2944204E-6	37.714283	23
ATAACGG	695	0.0	37.669064	2
GACCGAT	1725	0.0	37.623188	9
GGCGATA	1310	0.0	37.450382	8
>>END_MODULE