FastQCFastQC Report
Thu 26 May 2016
SRR1547148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547148_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5110745
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195560.3826448003177619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT161450.039.830291
TATCGCG2250.038.133331
CGACGGT7700.037.14285328
AGGGCGA39300.037.1145066
CGTAAGG7300.037.0684932
GGGCGAT80150.036.9457247
CTCACGA7700.036.8571424
TGGGCGA24700.036.6963546
TGTAGCG4200.036.6666641
TCGATAG2650.036.52831
GACCGAT25850.036.510649
CGGTCTA7500.036.0831
TACGGGA18550.035.816714
CGACCCG5100.035.8039232
GCGCGAC5600.035.759
CACGACG7700.035.71428326
TAAGGGA64350.035.658124
TATAGCG2350.035.574471
TAGGGAC54000.035.5666665
GGACCGA28200.035.4184388
Produced by FastQC (version 0.11.5)